Study on potential role of apolipoprotein E in recurrent pregnancy loss

Korkmazer,Engin; Ustunyurt,Emın; Tekin,Başar; Cilingir,Oguz

doi:10.3892/etm.2013.997

Study on potential role of apolipoprotein E in recurrent pregnancy loss

Authors:
- Engin Korkmazer
- Emın Ustunyurt
- Başar Tekin
- Oguz Cilingir
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Affiliations: Department of Obstetrics and Gynecology, Faculty of Medicine, Eskişehir Osmangazi University, Eskisehir, Turkey, Department of Obstetrics and Gynecology, Bursa Şevket Yılmaz Research and Education Hospital, Bursa, Turkey, Department of Medical Genetics, Eskişehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
Published online on: March 11, 2013 https://doi.org/10.3892/etm.2013.997
Pages: 1408-1410

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Abstract

The role of apolipoprotein E (Apo E) gene polymorphisms in the etiology of recurrent pregnancy loss (RPL) is not clearly understood. We evaluated this polymorphism in unexplained pregnancy losses in a group of Turkish women. In our prospective case-control study, 45 well-characterized RPL cases were examined for their Apo E genotypes, based on restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified fragments. The observed genotypes were compared with those obtained from equal number matched controls. We observed similar Apo E genotypes and E2, E3 and E4 allele frequency distribution among RPL patients and controls. The allele frequencies obtained in patients and controls, respectively, were as follows: E2=8 (9%) and 12 (13.4%) (P=0.342), E3=66 (73.3%) and 60 (66.6%) (P=0.328) and E4=16 (17.7%) and 18 (20%) (P=0.703). Our data did not support the association of Apo E gene polymorphisms with RPL as reported by previous studies. We endorse adequate characterization of RPL cases and adequate sample size prior to addressing such studies.

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1.	Ford HB and Schust DJ: Recurrent pregnancy loss: etiology, diagnosis, and therapy. Rev Obstet Gynecol. 2:76–83. 2009.PubMed/NCBI
2.	Mahley RW: Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science. 240:622–630. 1988. View Article : Google Scholar : PubMed/NCBI
3.	Eichner JE, Dunn ST, Perveen G, Thompson DM, Stewart KE and Stroehla BC: Apolipoprotein E polymorphism and cardiovascular disease: a HuGE review. Am J Epidemiol. 155:487–495. 2002. View Article : Google Scholar : PubMed/NCBI
4.	Bennet AM, Di Angelantonio E, Ye Z, Wensley F, et al: Association of apolipoprotein E genotypes with lipid levels and coronary risk. JAMA. 298:1300–1311. 2007. View Article : Google Scholar : PubMed/NCBI
5.	Atkinson KR, Blumenstein M, Black MA, et al: An altered pattern of circulating apolipoprotein E3 isoforms is implicated in preeclampsia. J Lipid Res. 501:71–80. 2009.PubMed/NCBI
6.	Goodman C, Goodman CS, Hur J, Jeyendran RS and Coulam C: The association of Apoprotein E polymorphisms with recurrent pregnancy loss. Am J Reprod Immunol. 61:34–38. 2009. View Article : Google Scholar : PubMed/NCBI
7.	Ergin E, Jeyendran RS, Ozornek H, Ozay A, Pillai MD and Coulam C: Is apolipoprotein E codon 112 polymorphism associated with recurrent pregnancy loss? Fertil Steril. 92:1152009. View Article : Google Scholar
8.	Bianca S, Barrano B, Cutuli N, et al: No association between apolipoprotein E polymorphisms and recurrent pregnancy loss. Fertil Steril. 93:2762010. View Article : Google Scholar : PubMed/NCBI
9.	Shi J, Zhao CB, Vollmer TL, Tyry TM and Kuniyoshi SM: APOE epsilon 4 allele is associated with cognitive impairment patients with multiple sclerosis. Neurology. 70:185–190. 2008. View Article : Google Scholar
10.	Huang Y, Weisgraber KH, Mucke L and Mahley RW: Apolipo-protein E: diversity of cellular origins, structural and biophysical properties, and effects in Alzheimer’s disease. J Mol Neurosci. 23:189–204. 2004.PubMed/NCBI
11.	Libby P: Current concepts of the pathogenesis of the acute coronary syndromes. Circulation. 104:365–372. 2001. View Article : Google Scholar : PubMed/NCBI
12.	Lynch JR, Tang W, Wang H, et al: APOE genotype and an ApoE-mimetic peptide modify the systemic and central nervous system inflammatory response. J Biol Chem. 278:48529–48533. 2003. View Article : Google Scholar : PubMed/NCBI
13.	Greer IA: The challenge of thrombophilia in maternal-fetal medicine. N Engl J Med. 342:424–425. 2000. View Article : Google Scholar : PubMed/NCBI
14.	Coulam CB, Jeyendran RS, Fishel LA and Roussev R: Multiple thrombophilic gene mutations rather than specific gene mutations are risk factors for recurrent miscarriage. Am J Reprod Immunol. 55:360–368. 2006. View Article : Google Scholar : PubMed/NCBI
15.	Corbo RM, Scacchi R and Cresta M: Differential reproductive efficiency associated with common apolipoprotein e alleles in postreproductive-aged subjects. Fertil Steril. 81:104–107. 2004. View Article : Google Scholar : PubMed/NCBI
16.	Zetterberg H, Palmér M, Ricksten A, et al: Influence of the apolipoprotein E epsilon4 allele on human embryonic development. Neurosci Lett. 324:189–192. 2002. View Article : Google Scholar : PubMed/NCBI
17.	Makkonen N, Heinonen S, Hiltunen M, Helisalmi S, Mannermaa A and Kirkinen P: Apolipoprotein E alleles in women with pre-eclampsia. J Clin Pathol. 54:652–654. 2001. View Article : Google Scholar : PubMed/NCBI
18.	Yenicesu GI, Cetin M, Ozdemir O, et al: A prospective case-control study analyzes 12 thrombophilic gene mutations in Turkish couples with recurrent pregnancy loss. Am J Reprod Immunol. 63:126–36. 2010. View Article : Google Scholar : PubMed/NCBI