Inhibition of Aurora‑B suppresses osteosarcoma cell migration and invasion

  • Authors:
    • Xiao Ping Zhu
    • Zhi Li Liu
    • Ai Fen Peng
    • Yun Fei Zhou
    • Xin Hua Long
    • Qing Feng Luo
    • Shan Hu Huang
    • Yong Shu
  • View Affiliations

  • Published online on: January 20, 2014     https://doi.org/10.3892/etm.2014.1491
  • Pages: 560-564
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Previous studies have suggested that Aurora‑B may be involved in cancer metastasis. However, its role has been poorly evaluated in osteosarcoma (OS). The aim of this study was to investigate the correlation between Aurora‑B expression and metastasis in human OS. The human OS cell line, U2‑OS, and OS biopsy specimens were used in the study. The expression of Aurora‑B protein was examined using immunohistochemistry and western blotting in OS tissues and U2‑OS cells, respectively. AZD1152‑hydroxyquinazoline‑pyrazol‑anilide, an inhibitor of Aurora‑B, was used to inhibit Aurora‑B expression in U2‑OS cells. The effect of Aurora‑B inhibition on U2‑OS cell proliferation, invasion and migration was assessed using MTT, colony formation, wound healing and Transwell assays. The results showed that positive expression of the Aurora‑B protein was observed in the nucleus, and that Aurora‑B expression levels in the cases with pulmonary metastases were significantly higher than in those without metastasis. In vitro, the proliferation, invasion and migration of U2‑OS cells were suppressed by the inhibition of Aurora‑B. These results suggest that Aurora‑B may be involved in OS metastasis, and may be a promising target in the treatment of OS metastasis.
View Figures
View References

Related Articles

Journal Cover

2014-March
Volume 7 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu XP, Liu ZL, Peng AF, Zhou YF, Long XH, Luo QF, Huang SH and Shu Y: Inhibition of Aurora‑B suppresses osteosarcoma cell migration and invasion. Exp Ther Med 7: 560-564, 2014.
APA
Zhu, X.P., Liu, Z.L., Peng, A.F., Zhou, Y.F., Long, X.H., Luo, Q.F. ... Shu, Y. (2014). Inhibition of Aurora‑B suppresses osteosarcoma cell migration and invasion. Experimental and Therapeutic Medicine, 7, 560-564. https://doi.org/10.3892/etm.2014.1491
MLA
Zhu, X. P., Liu, Z. L., Peng, A. F., Zhou, Y. F., Long, X. H., Luo, Q. F., Huang, S. H., Shu, Y."Inhibition of Aurora‑B suppresses osteosarcoma cell migration and invasion". Experimental and Therapeutic Medicine 7.3 (2014): 560-564.
Chicago
Zhu, X. P., Liu, Z. L., Peng, A. F., Zhou, Y. F., Long, X. H., Luo, Q. F., Huang, S. H., Shu, Y."Inhibition of Aurora‑B suppresses osteosarcoma cell migration and invasion". Experimental and Therapeutic Medicine 7, no. 3 (2014): 560-564. https://doi.org/10.3892/etm.2014.1491