Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
2014-April Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-April Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

In vitro anti‑MRSA activity of carvone with gentamicin

  • Authors:
    • Su‑Hyun Mun
    • Ok‑Hwa Kang
    • Dae‑Ki Joung
    • Sung‑Bae Kim
    • Jang‑Gi Choi
    • Dong‑Won Shin
    • Dong‑Yeul Kwon
  • View Affiliations / Copyright

    Affiliations: BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, BK21 Plus Program & Department of Smart Life-Care Convergence, Gradulate School, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Oriental Medicine Resources, College of Bio Industry Science, Sunchon National University, Sunchon, Jeonnam 540-742, Republic of Korea
  • Pages: 891-896
    |
    Published online on: January 23, 2014
       https://doi.org/10.3892/etm.2014.1498
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Carvone is one of the naturally occurring monoterpenes, the largest class of secondary metabolites in plants, and exists in two enantiomers, R‑carvone (R‑car) and S‑car. The objective of this study was to investigate the antimicrobial activity of R‑car and S‑car with gentamicin (GET) against methicillin‑resistant Staphylococcus aureus (MRSA). MRSA is a major human pathogen that causes serious problems, including hospital‑acquired pneumonia, abscesses and surgical wound infections. Nosocomial MRSA infections often exhibit multidrug resistance. In the present study, antimicrobial susceptibility testing was performed with R‑car, S‑car and GET using the broth microdilution method. Minimal inhibitory concentration values for R‑ and S‑car against six different strains of S. aureus ranged between 500 and 1,000 µg/ml. Anti‑MRSA activity was evaluated using the checkerboard and time‑kill assays to investigate the potential synergistic effects of different combinations of the carvone enantiomers and GET. R‑car plus S‑car, R‑car plus GET and S‑car plus GET exhibited significant synergistic activity against MRSA. These findings suggest that the single‑agent anti‑MRSA activities of R‑car, S‑car and GET are effectively increased through combination therapy. This study showed that carvone may be a potential adjuvant antimicrobial agent.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

de Carvalho CCCR and da Fonseca MMR: Carvone: Why and how should one bother to produce this terpene. Food Chem. 95:413–422. 2006.

2 

Grodner B and Sitkiewicz D: Enantiomers: a new problem in pharmacotherapy of depression? Psychiatr Pol. 47:511–518. 2013.(In Polish).

3 

Cho IJ, Lee CW, Lee MY, Kang MR, Yun J, Oh SJ, Han SB, Lee K, Park SK, Kim HM, et al: Differential anti-inflammatory and analgesic effects by enantiomers of zaltoprofen in rodents. Int Immunopharmacol. 16:457–460. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Krishnaiah YS and Nada A: Enantioselective penetration enhancing effect of carvone on the in vitro transdermal permeation of nicorandil. Pharm Dev Technol. 17:574–582. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Lehmann K, Crombie A and Singer AC: Reproducibility of a microbial river water community to self-organize upon perturbation with the natural chemical enantiomers, R- and S-carvone. FEMS Microbiol Ecol. 66:208–220. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Jäger W, Mayer M, Reznicek G and Buchbauer G: Percutaneous absorption of the montoterperne carvone: implication of stereoselective metabolism on blood levels. J Pharm Pharmacol. 53:637–642. 2001.PubMed/NCBI

7 

Jevons MP: ‘Celbenin’ - resistant Staphylococci. Br Med J. 1:124–125. 1961.

8 

Tsubakishita S, Kuwahara-Arai K, Sasaki T and Hiramatsu K: Origin and molecular evolution of the determinant of methicillin resistance in staphylococci. Antimicrob Agents Chemother. 54:4352–4359. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Ragle BE and Bubeck Wardenburg J: Anti-alpha-hemolysin monoclonal antibodies mediate protection against Staphylococcus aureus pneumonia. Infect Immun. 77:2712–2718. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Gurieva TV, Bootsma MC and Bonten MJ: Decolonization of patients and health care workers to control nosocomial spread of methicillin-resistant Staphylococcus aureus: a simulation study. BMC Infect Dis. 12:3022012. View Article : Google Scholar : PubMed/NCBI

11 

Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard. 7th edition. CLSI; Wayne, PA, USA: pp. M7–A7. 2006

12 

Chang SC, Chen YC, Luh KT and Hsieh WC: In vitro activities of antimicrobial agents, alone and in combination, against Acinetobacter baumannii isolated from blood. Diagn Microbiol Infect Dis. 23:105–110. 1995. View Article : Google Scholar : PubMed/NCBI

13 

Noble WC, Virani Z and Cree RG: Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett. 72:195–198. 1992. View Article : Google Scholar : PubMed/NCBI

14 

Timurkaynak F, Can F, Azap OK, Demirbilek M, Arslan H and Karaman SO: In vitro activities of non-traditional antimicrobials alone or in combination against multidrug-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii isolated from intensive care units. Int J Antimicrob Agents. 27:224–228. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Moellering RC Jr: Discovering new antimicrobial agents. Int J Antimicrob Agents. 37:2–9. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Jung HJ and Lee DG: Synergistic antibacterial effect between silybin and N,N′-dicyclohexylcarbodiimide in clinical Pseudomonas aeruginosa isolates. J Microbiol. 46:462–467. 2008.PubMed/NCBI

17 

Müller P, Alber DG, Turnbull L, Schlothauer RC, Carter DA, Whitchurch CB and Harry EJ: Synergism between Medihoney and rifampicin against methicillin-resistant Staphylococcus aureus (MRSA). PLoS One. 8:e576792013.PubMed/NCBI

18 

Celenza G, Segatore B, Setacci D, Bellio P, Brisdelli F, Piovano M, Garbarino JA, Nicoletti M, Perilli M and Amicosante G: In vitro antimicrobial activity of pannarin alone and in combination with antibiotics against methicillin-resistant Staphylococcus aureus clinical isolates. Phytomedicine. 19:596–602. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Mun SH, Joung DK, Kim YS, Kang OH, Kim SB, Seo YS, Kim YC, Lee DS, Shin DW, Kweon KT and Kwon DY: Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine. 20:714–718. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Nester EW, Roberts CE, Pearsall NN, Anderson DG and Nester MT: Cell walls of gram-positive bacteria, cell walls of gram-negative bacteria. Microbiology: A Human Perspective. Nester EW, Roberts CE and Nester MT: 2nd edition. WCB/McGraw-Hill; Columbus, OH: pp. 58–59. 1998

21 

Uribe S, Ramirez J and Peña A: Effects of beta-pinene on yeast membrane functions. J Bacteriol. 161:1195–1200. 1985.PubMed/NCBI

22 

Sikkema J, de Bont JA and Poolman B: Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev. 59:201–222. 1995.

23 

Denyer SP and Hugo WB: Biocide-induced damage to the bacterial cytoplasmic membrane. Mechanisms of Action of Chemical Biocides: Their Study and Exploitation. Blackwell Scientific Publications; Oxford: pp. 171–187. 1991

24 

Otto CC, Cunningham TM, Hansen MR and Haydel SE: Effects of antibacterial mineral leachates on the cellular ultrastructure, morphology, and membrane integrity of Escherichia coli and methicillin-resistant Staphylococcus aureus. Ann Clin Microbiol Antimicrob. 9:262010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Mun SH, Kang OH, Joung DK, Kim SB, Choi JG, Shin DW and Kwon DY: In vitro anti‑MRSA activity of carvone with gentamicin. Exp Ther Med 7: 891-896, 2014.
APA
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., & Kwon, D. (2014). In vitro anti‑MRSA activity of carvone with gentamicin. Experimental and Therapeutic Medicine, 7, 891-896. https://doi.org/10.3892/etm.2014.1498
MLA
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., Kwon, D."In vitro anti‑MRSA activity of carvone with gentamicin". Experimental and Therapeutic Medicine 7.4 (2014): 891-896.
Chicago
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., Kwon, D."In vitro anti‑MRSA activity of carvone with gentamicin". Experimental and Therapeutic Medicine 7, no. 4 (2014): 891-896. https://doi.org/10.3892/etm.2014.1498
Copy and paste a formatted citation
x
Spandidos Publications style
Mun SH, Kang OH, Joung DK, Kim SB, Choi JG, Shin DW and Kwon DY: In vitro anti‑MRSA activity of carvone with gentamicin. Exp Ther Med 7: 891-896, 2014.
APA
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., & Kwon, D. (2014). In vitro anti‑MRSA activity of carvone with gentamicin. Experimental and Therapeutic Medicine, 7, 891-896. https://doi.org/10.3892/etm.2014.1498
MLA
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., Kwon, D."In vitro anti‑MRSA activity of carvone with gentamicin". Experimental and Therapeutic Medicine 7.4 (2014): 891-896.
Chicago
Mun, S., Kang, O., Joung, D., Kim, S., Choi, J., Shin, D., Kwon, D."In vitro anti‑MRSA activity of carvone with gentamicin". Experimental and Therapeutic Medicine 7, no. 4 (2014): 891-896. https://doi.org/10.3892/etm.2014.1498
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team