Correlation between single nucleotide polymorphisms of the ACTA2 gene and coronary artery stenosis in patients with type 2 diabetes mellitus

Fang,Hui; Luo,Xiaoli; Wang,Yan; Liu,Nian; Fu,Chunjiang; Wang,Hongyong; Fang,Yuqiang; Shi,Weibin; Zhang,Ye; Zeng,Chunyu; Wang,Xukai

doi:10.3892/etm.2014.1510

2014-April Volume 7 Issue 4

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2014-April Volume 7 Issue 4

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Article

Correlation between single nucleotide polymorphisms of the ACTA2 gene and coronary artery stenosis in patients with type 2 diabetes mellitus

Authors:
- Hui Fang
- Xiaoli Luo
- Yan Wang
- Nian Liu
- Chunjiang Fu
- Hongyong Wang
- Yuqiang Fang
- Weibin Shi
- Ye Zhang
- Chunyu Zeng
- Xukai Wang
View Affiliations / Copyright

Affiliations: Department of Cardiology, Institute of War Surgery, The Third Affiliated Hospital of Third Military Medical University, Chongqing 400042, P.R. China, Department of Medical Genetics, Third Military Medical University, Chongqing 400038, P.R. China
Pages: 970-976
|
Published online on: January 28, 2014

https://doi.org/10.3892/etm.2014.1510
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Abstract

This study aimed to analyze the correlation between single nucleotide polymorphisms (SNPs) of the actin, aortic smooth muscle (ACTA2) gene and coronary artery stenosis in patients with type 2 diabetes mellitus (T2DM). Eight SNPs from the promoter region of the ACTA2 gene were screened. Patients with T2DM (n=251) were divided into two groups, those with severe coronary stenosis (SCS+ group; n=168) and those without severe coronary stenosis (SCS- group; n=83). Patients were also divided according to lesion branching into those whose lesions involved less than three branches (LCA- group) and those whose lesions involved at least three branches (LCA+ group). The clinical and laboratory data of the patients were collected, and the genotyping of eight SNPs was conducted followed by statistical analysis. Of the eight SNPs, only the rs1324551 SNP was identified to be significantly different between the SCS+ and SCS- groups (P<0.05). The frequency of the rs1324551 G allele and GG genotype in the SCS+ group was significantly higher than that of the SCS- group (P=0.044 and P=0.001, respectively). No significant difference was observed between the LCA- and LCA+ groups. Following the deduction of age, gender and traditional risk factors, the odds ratios of the GG genotype in additive and recessive models were 2.93 [95% confidence interval (CI), 1.05-8.19; P=0.04] and 2.34 (95% CI, 1.09-5.02; P=0.03), respectively, and this relevancy was represented only in patients with low insulin levels. Age and smoking were also found to increase the risk of coronary artery lesions. In conclusion, the rs1324551 SNP in the promoter region of the ACTA2 gene was identified to be independently correlated with the degree of coronary artery stenosis in patients with T2DM and plasma insulin may inhibit coronary artery stenosis during the pathogenic process.

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1	Morrish NJ, Wang SL, Stevens LK, Fuller JH and Keen H: Mortality and causes of death in the WHO Multinational Study of Vascular Disease in Diabetes. Diabetologia. 44(Suppl 2): S14–S21. 2001. View Article : Google Scholar : PubMed/NCBI
2	Critchley J, Liu J, Zhao D, Wei W and Capewell S: Explaining the increase in coronary heart disease mortality in Beijing between 1984 and 1999. Circulation. 110:1236–1244. 2004. View Article : Google Scholar : PubMed/NCBI
3	Fox CS, Coady S, Sorlie PD, et al: Increasing cardiovascular disease burden due to diabetes mellitus: the Framingham Heart Study. Circulation. 115:1544–1550. 2007. View Article : Google Scholar : PubMed/NCBI
4	Breen DM and Giacca A: Effects of insulin on the vasculature. Curr Vasc Pharmacol. 9:321–332. 2011. View Article : Google Scholar : PubMed/NCBI
5	Defronzo RA: Is insulin resistance atherogenic? Possible mechanisms. Atheroscler Suppl. 7:11–15. 2006. View Article : Google Scholar : PubMed/NCBI
6	Bornfeldt KE and Tabas I: Insulin resistance, hyperglycemia, and atherosclerosis. Cell Metab. 14:575–585. 2011. View Article : Google Scholar : PubMed/NCBI
7	Akin I, Bufe A, Eckardt L, et al: Comparison of outcomes in patients with insulin-dependent versus non-insulin dependent diabetes mellitus receiving drug-eluting stents (from the first phase of the prospective multicenter German DES. DE registry). Am J Cardiol. 106:1201–1207. 2010. View Article : Google Scholar
8	BARI 2D Study Group. Frye RL, August P, Brooks MM, et al: A randomized trial of therapies for type 2 diabetes and coronary artery disease. N Engl J Med. 360:2503–2515. 2009. View Article : Google Scholar : PubMed/NCBI
9	Pop-Busui R, Lombardero M, Lavis V, et al; Bari 2D Study Group. Relation of severe coronary artery narrowing to insulin or thiazolidinedione use in patients with type 2 diabetes mellitus (from the Bypass Angioplasty Revascularization Investigation 2 Diabetes Study). Am J Cardiol. 104:52–58. 2009. View Article : Google Scholar
10	Wang Y, Zhang B, Bai Y, Zeng C and Wang X: Changes in proteomic features induced by insulin on vascular smooth muscle cells from spontaneous hypertensive rats in vitro. Cell Biochem Biophys. 58:97–106. 2010. View Article : Google Scholar : PubMed/NCBI
11	Rzucidlo EM, Martin KA and Powell RJ: Regulation of vascular smooth muscle cell differentiation. J Vasc Surg. 45(Suppl A): A25–A32. 2007. View Article : Google Scholar
12	Orr AW, Hastings NE, Blackman BR and Wamhoff BR: Complex regulation and function of the inflammatory smooth muscle cell phenotype in atherosclerosis. J Vasc Res. 47:168–180. 2010. View Article : Google Scholar : PubMed/NCBI
13	Guo DC, Papke CL, Tran-Fadulu V, et al: Mutations in smooth muscle alpha-actin (ACTA2) cause coronary artery disease, stroke, and Moyamoya disease, along with thoracic aortic disease. Am J Hum Genet. 84:617–627. 2009. View Article : Google Scholar : PubMed/NCBI
14	Guo DC, Pannu H, Tran-Fadulu V, et al: Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections. Nat Genet. 39:1488–1493. 2007. View Article : Google Scholar : PubMed/NCBI
15	Sobti RC, Kler R, Sharma YP, Talwar KK and Singh N: Risk of obesity and type 2 diabetes with tumor necrosis factor-α 308G/A gene polymorphism in metabolic syndrome and coronary artery disease subjects. Mol Cell Biochem. 360:1–7. 2012.
16	Al-Daghri NM, Al-Attas OS, Alokail MS, Alkharfy KM and Hussain T: Adiponectin gene variants and the risk of coronary artery disease in patients with type 2 diabetes. Mol Biol Rep. 38:3703–3708. 2011. View Article : Google Scholar : PubMed/NCBI
17	Funnell MM: Standards of care for diabetes: what’s new? Nursing. 40:54–56. 2010.
18	Muniyappa R, Lee S, Chen H and Quon MJ: Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. Am J Physiol Endocrinol Metab. 294:15–26. 2008. View Article : Google Scholar : PubMed/NCBI
19	Nguyen AB, Rohatgi A, Garcia CK, et al: Interactions between smoking, pulmonary surfactant protein B, and atherosclerosis in the general population: the Dallas Heart Study. Arterioscler Thromb Vasc Biol. 31:2136–2143. 2011. View Article : Google Scholar : PubMed/NCBI
20	Kohsaka S, Jin Z, Rundek T, Homma S, Sacco RL and Di Tullio MR: Alcohol consumption and atherosclerotic burden in the proximal thoracic aorta. Atherosclerosis. 219:794–798. 2011. View Article : Google Scholar : PubMed/NCBI
21	Wu TG and Wang L: Angiographic characteristics of the coronary artery in patients with type 2 diabetes. Exp Clin Cardiol. 7:199–200. 2002.PubMed/NCBI
22	Athyros VG, Tziomalos K, Katsiki N, et al; GREACE Study Collaborative Group. The impact of smoking on cardiovascular outcomes and comorbidities in statin-treated patients with coronary artery disease: a post hoc analysis of the GREACE Study. Curr Vasc Pharmacol. 11:779–784. 2013. View Article : Google Scholar : PubMed/NCBI
23	Cheng JL and Lee SY: Biological aging and coronary artery disease. Anadolu Kardiyol Derg. 12:4642012.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Correlation between single nucleotide polymorphisms of the ACTA2 gene and coronary artery stenosis in patients with type 2 diabetes mellitus

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