Prevention of amiodarone‑induced cardiac toxicity in male BALB/c mice by a nutrient mixture

Roomi,M. Waheed; Roomi,Nusrath Waheed; Kalinovsky,Tatiana; Rath,Matthias; Niedzwiecki,Aleksandra

doi:10.3892/etm.2014.1518

2014-April Volume 7 Issue 4

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2014-April Volume 7 Issue 4

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Article

Prevention of amiodarone‑induced cardiac toxicity in male BALB/c mice by a nutrient mixture

Authors:
- M. Waheed Roomi
- Nusrath Waheed Roomi
- Tatiana Kalinovsky
- Matthias Rath
- Aleksandra Niedzwiecki
View Affiliations / Copyright

Affiliations: Dr. Rath Research Institute, Santa Clara, CA 95050, USA
Pages: 987-989
|
Published online on: February 3, 2014

https://doi.org/10.3892/etm.2014.1518
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Abstract

Amiodarone (Amio), a potent anti‑arrhythmic drug, is associated with life‑threatening pulmonary toxicity involving fibroses and inflammation. A unique nutrient mixture (NM) consisting of lysine, proline, ascorbic acid, N‑acetyl cysteine and green tea extract has previously been shown to exhibit a broad spectrum of pharmacological, therapeutic, cardiovascular and chemopreventive properties. The present study was undertaken to determine whether the NM exhibits preventive effects on Amio‑induced cardiac toxicity. Six‑week‑old male BALB/c mice were divided into four groups (A‑D) of six animals per group. Mice in groups A and C were fed a regular diet for three weeks, while the diets of the mice in groups B and D were supplemented with 1% NM during that period. After three weeks, the mice in groups C and D received daily Amio injections of 50 mg/kg body weight intraperitoneally for 4 days, whilst those in groups A and B received saline alone. At 24 h after the final dose, mice were sacrificed, blood was withdrawn and serum was collected for clinical chemistry of the heart enzymes creatine phosphokinase (CPK) and aspartate aminotransferase (AST). In addition, livers, kidneys, hearts and lungs were excised and weighed. No significant differences in weight gain were identified among the groups and liver, kidney, heart and lung weights were comparable in all four groups. Administration of Amio to group C resulted in a significant increase in serum CPK levels, whereas in NM‑fed group D, the CPK levels were comparable to those in the saline injection groups, A and B. Amio administration also resulted in a significant increase in serum AST levels in group C, but not in the group D animals which exhibited similar levels to those of groups A and B. Therefore, the results indicate that NM has the potential to protect against Amio‑induced cardiac toxicity.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Prevention of amiodarone‑induced cardiac toxicity in male BALB/c mice by a nutrient mixture

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