FIZZ1 promotes airway remodeling through the PI3K/Akt signaling pathway in asthma

Wang,Junfei; Li,Fei; Yang,Mengmeng; Wu,Jinxiang; Zhao,Jiping; Gong,Wenbin; Liu,Wen; Bi,Wenxiang; Dong,Liang

doi:10.3892/etm.2014.1580

FIZZ1 promotes airway remodeling through the PI3K/Akt signaling pathway in asthma

Authors:
- Junfei Wang
- Fei Li
- Mengmeng Yang
- Jinxiang Wu
- Jiping Zhao
- Wenbin Gong
- Wen Liu
- Wenxiang Bi
- Liang Dong
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Affiliations: Department of Pulmonary Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Pulmonary Medicine, The Second Hospital of Shandong University, Jinan, Shandong 250100, P.R. China, Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: February 24, 2014 https://doi.org/10.3892/etm.2014.1580
Pages: 1265-1270

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Abstract

Found in inflammatory zone 1 (FIZZ1) plays a vital role in pulmonary inflammation and angiogenesis. In addition, FIZZ1 plays a role in the early stages of airway remodeling in asthma by increasing the expression of α smooth muscle actin (α‑SMA) and type I collagen. However, the role of FIZZ1 in the airway remodeling of asthma remains unclear. In the present study, FIZZ1 was identified to be upregulated in ovalbumin (OVA)‑induced asthmatic mice, along with phosphorylated protein kinase B (Akt). Following FIZZ1 recombinant protein co‑culture in the murine lung epithelial‑cell line, Akt phosphorylation was upregulated, however, following transfection with FIZZ1‑small hairpin RNA, the phosphorylation levels were decreased. The variation in α‑SMA and type I collagen expression levels was consistent with the Akt phosphorylation levels. Intratracheal administration of LY294002 and Akt inhibitor IV to the asthmatic mice was capable of reducing airway inflammation, downregulating the expression of α‑SMA, type I collagen and fibronectin‑1 and increasing the expression of E‑cadherin. In conclusion, the present study demonstrated that FIZZ1 promoted airway remodeling in asthma via the phosphoinositide 3‑kinase (PI3K)/Akt signaling pathway. Blocking the PI3K/Akt signaling pathway may attenuate the early stages of airway remodeling induced by OVA by regulating the abnormal process of epithelial‑mesenchymal transition.

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