Chloral hydrate‑dependent reduction in the peptidoglycan‑induced inflammatory macrophage response is associated with lower expression levels of toll‑like receptor 2

Pan,Qingjun; Liu,Yuan; Zhu,Xuezhi; Liu,Huafeng

doi:10.3892/etm.2014.1587

Chloral hydrate‑dependent reduction in the peptidoglycan‑induced inflammatory macrophage response is associated with lower expression levels of toll‑like receptor 2

Authors:
- Qingjun Pan
- Yuan Liu
- Xuezhi Zhu
- Huafeng Liu
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Affiliations: Institute of Nephrology, Affiliated Hospital of Guangdong Medical College, Zhangjiang, Guangdong 524001, P.R. China, Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, SAR 999077, P.R. China, Guangdong Yuehai Feed Group Co. Ltd., Zhangjiang, Guangdong 524001, P.R. China
Published online on: February 26, 2014 https://doi.org/10.3892/etm.2014.1587
Pages: 1305-1310

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Abstract

The aim of this study was to investigate the effect and mechanism of action of chloral hydrate on the peptidoglycan (PGN)‑induced inflammatory macrophage response. The effect of chloral hydrate on the production of tumor necrosis factor α (TNF‑α) and interleukin‑6 (IL‑6) by murine peritoneal macrophages with PGN‑stimulation was investigated. In addition, RAW264.7 cells transfected with a nuclear factor‑κB (NF‑κB) luciferase reporter plasmid stimulated by PGN were used to study the effect of chloral hydrate on the levels NF‑κB activity. Flow cytometry and western blotting were performed to investigate the expression levels of toll‑like receptor 2 (TLR2) in the treated RAW264.7 cells. It was identified that chloral hydrate reduced the levels of IL‑6 and TNF‑α produced by the peritoneal macrophages stimulated with PGN. The levels of NF‑κB activity of the RAW264.7 cells stimulated by PGN decreased following treatment with chloral hydrate, which was associated with a reduction in the expression levels of TLR2 and reduced levels of TLR2 signal transduction. These data demonstrate that chloral hydrate reduced the magnitude of the PGN‑induced inflammatory macrophage response associated with lower expression levels of TLR2.

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