Effect of tissue factor knockdown on the growth, invasion, chemoresistance and apoptosis of human gastric cancer cells

Yu,Yong‑Jiang; Hou,Xu‑Dong; Li,Yu‑Min

doi:10.3892/etm.2014.1591

Effect of tissue factor knockdown on the growth, invasion, chemoresistance and apoptosis of human gastric cancer cells

Authors:
- Yong‑Jiang Yu
- Xu‑Dong Hou
- Yu‑Min Li
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Affiliations: Department of General Surgery, The 1st Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of General Surgery, The 2nd Hospital of Lanzhou University, Lanzhou, Gansu 730020, P.R. China
Published online on: February 28, 2014 https://doi.org/10.3892/etm.2014.1591
Pages: 1376-1382

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Abstract

This study aimed to explore the role of tissue factor (TF) and evaluate its antitumor effects in the biological processes of gastric cancer cells using the application of RNA interference technology to silence TF in the SGC7901 gastric cancer cell line. Specific small interfering RNA (siRNA) designed for targeting human TF was transfected into SGC7901 cells. The expression levels of TF in the cells were detected by reverse transcription‑polymerase chain reaction. Cell proliferation and chemosensitivity were measured by Cell Counting Kit‑8. The metastatic potential of the SGC7901 cells was determined by Transwell experiments and wound‑healing assays. Cell apoptosis was assessed by Annexin V‑fluorescein isothiocyanate/propidium iodide double‑staining method. The expression levels of TF mRNA were significantly reduced by the TF‑siRNA in the SGC7901 cells, resulting in the suppression of cell proliferation, chemoresistance and invasion, and subsequently the induction of cell apoptosis. TF knockdown with siRNA inhibits the growth, invasion and chemoresistance and enhances the apoptosis of SGC7901 cells, providing a potential approach for gene therapy against human gastric cancer.

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