Pseudomonas aeruginosa inhibits the growth of pathogenic fungi: In vitro and in vivo studies

Xu,Lingqing; Wang,Feng; Shen,Yin; Hou,Hongyan; Liu,Weiyong; Liu,Cailin; Jian,Cui; Wang,Yue; Sun,Mingyue; Sun,Ziyong

doi:10.3892/etm.2014.1631

June-2014 Volume 7 Issue 6

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June-2014 Volume 7 Issue 6

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Article

Pseudomonas aeruginosa inhibits the growth of pathogenic fungi: In vitro and in vivo studies

Authors:
- Lingqing Xu
- Feng Wang
- Yin Shen
- Hongyan Hou
- Weiyong Liu
- Cailin Liu
- Cui Jian
- Yue Wang
- Mingyue Sun
- Ziyong Sun
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
Pages: 1516-1520
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Published online on: March 21, 2014

https://doi.org/10.3892/etm.2014.1631
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Abstract

The aim of the present study was to investigate the inhibitory effect of Pseudomonas aeruginosa (PA) on pathogenic fungi, including Candida albicans (CA), Candida tropicalis (CT), Candida glabrata (CG), Candida parapsilosis (CP) and Candida krusei (CK), in vitro and in vivo. In total, 24 PA strains were collected from clinical specimens and identified by Gram staining, oxidase production and the API 20NE system. Cross‑streak, disk diffusion and co‑culture methods were used to observe the inhibitory effect of PA. Sodium dodecyl sulfate‑polyacrylamide gel electrophoresis was used to analyze differences in the bacterial proteins of PA. A blood infection model in mice was used to evaluate the effect of PA on fungi in vivo. The in vitro and in vivo results demonstrated that a number of PA isolates exhibited a marked inhibitory effect on pathogenic fungi, including CA, CT, CP, CG and CK, while other PA strains exhibited no effect. Therefore, PA exhibits an inhibitory effect on pathogenic fungi and this activity may be important in the treatment of patients. It was hypothesized that PA secretes various types of proteins to suppress the growth of fungal filaments, which subsequently inhibits pathogenic fungi.

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1	Fenical W: Chemical studies of marine bacteria: developing a new resource. Chem Rev. 93:1673–1683. 1993.
2	Tawiah AA, Gbedema SY, Adu F, et al: Antibiotic producing microorganisms from River Wiwi, Lake Bosomtwe and the Gulf of Guinea at Doakor Sea Beach, Ghana. BMC Microbiol. 12:2342012. View Article : Google Scholar : PubMed/NCBI
3	Singer RS, Finch R, Wegener HC, Bywater R, Walters J and Lipsitch M: Antibiotic resistance - the interplay between antibiotic use in animals and human beings. Lancet Infect Dis. 3:47–51. 2003. View Article : Google Scholar : PubMed/NCBI
4	Bhavnani SM and Ballow CH: New agents for Gram-positive bacteria. Curr Opin Microbiol. 3:528–534. 2000. View Article : Google Scholar : PubMed/NCBI
5	Kandela SA, al-Shibib AS and al-Khayat BH: A study of purified pyorubin produced by local Pseudomonas aeruginosa. Acta Microbiol Pol. 1:37–43. 1997.PubMed/NCBI
6	Kerr JR, Taylor GW, Rutman A, Høiby N, Cole PJ and Wilson R: Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth. J Clin Pathol. 52:385–387. 1999. View Article : Google Scholar
7	Hughes WT and Kim HK: Mycoflora in cystic fibrosis: some ecologic aspects of Pseudomonas aeruginosa and Candida albicans. Mycopathol Mycol Appl. 50:261–269. 1973. View Article : Google Scholar : PubMed/NCBI
8	Taylor GW, Machan ZA, Mehmet S, Cole PJ and Wilson R: Rapid identification of 4-hydroxy-2-alkylquinolines produced by Pseudomonas aeruginosa using gas chromatography-electron-capture mass spectrometry. J Chromatogr B Biomed Appl. 664:458–462. 1995. View Article : Google Scholar : PubMed/NCBI
9	Caldwell CC, Chen Y, Goetzmann HS, Hao Y, Borchers MT, Hassett DJ, et al: Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis. Am J Pathol. 175:2473–2488. 2009. View Article : Google Scholar : PubMed/NCBI
10	Kerr JR: Suppression of fungal growth exhibited by Pseudomonas aeruginosa. J Clin Microbiol. 32:525–527. 1994.PubMed/NCBI
11	Hogan DA and Kolter R: Pseudomonas-Candida interactions: an ecological role for virulence factors. Science. 296:2229–2232. 2002. View Article : Google Scholar : PubMed/NCBI
12	Rella A, Yang MW, Gruber J, et al: Pseudomonas aeruginosa inhibits the growth of Cryptococcus species. Mycopathologia. 173:451–461. 2012. View Article : Google Scholar
13	Teoh-Chan H, Chau PY, Ng MH and Wong PC: Inhibition of Cryptococcus neoformans by Pseudomonas aeruginosa. J Med Microbiol. 8:77–81. 1975.
14	Grillot R, Portmann-Coffin V and Ambroise-Thomas P: Growth inhibition of pathogenic yeasts by Pseudomonas aeruginosa in vitro: clinical implications in blood cultures. Mycoses. 37:343–347. 1994.PubMed/NCBI
15	Foster KW, Thomas L, Warner J, Desmond R and Elewski BE: A bipartite interaction between Pseudomonas aeruginosa and fungi in onychomycosis. Arch Dermatol. 141:1467–1468. 2005. View Article : Google Scholar : PubMed/NCBI
16	Hockey LJ, Fujita NK, Gibson TR, Montgomerie JZ and Edwards JE Jr: Detection of fungemia obscured by concomitant bacteremia: in vitro and in vivo studies. J Clin Microbiol. 16:1080–1085. 1982.PubMed/NCBI
17	Gupta N, Haque A, Mukhopadhyay G, Narayan RP and Prasad R: Interactions between bacteria and Candida in the burn wound. Burns. 31:375–378. 2005. View Article : Google Scholar
18	Neely AN, Law EJ and Holder IA: Increased susceptibility to lethal Candida infections in burned mice preinfected with Pseudomonas aeruginosa or pretreated with proteolytic enzymes. Infect Immun. 52:200–204. 1986.