Effect of erythropoietin loading chitosan‑tripolyphosphate nanoparticles on an IgA nephropathy rat model

Zhang,Xiaoli; Wu,Yin; Sun,Kun; Tan,Jing

doi:10.3892/etm.2014.1643

June-2014 Volume 7 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

June-2014 Volume 7 Issue 6

Full Size Image

Article

Effect of erythropoietin loading chitosan‑tripolyphosphate nanoparticles on an IgA nephropathy rat model

Authors:
- Xiaoli Zhang
- Yin Wu
- Kun Sun
- Jing Tan
View Affiliations / Copyright

Affiliations: Department of Rheumatism and Nephropathy, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 1659-1662
|
Published online on: March 28, 2014

https://doi.org/10.3892/etm.2014.1643
Expand metrics +

Abstract

The aim of the present study was to investigate the effect of erythropoietin (EPO) loading chitosan‑tripolyphosphate (CS‑TPP) nanoparticles on an immunoglobulin A nephropathy (IgAN) rat model. CS‑TPP nanoparticles were produced from CS and TPP and EPO was loaded by mixing with the nanoparticles. The IgAN rat models were randomly divided into three groups: the CS‑TPP‑EPO group, CS‑TPP group and EPO group. Hemoglobin (Hb), blood urea nitrogen (BUN) and creatinine (Cr) levels were measured in each group using a Biochemical Analyzer (Hitachi, Tokyo, Japan). The average size of nanoparticles was 485±12 nm and the encapsulation efficiency of EPO was 78.45%. The EPO release curve in CS‑TPP‑EPO nanoparticles exhibited a biphasic distribution in vitro. The levels of BUN and Cr in the CS‑TPP‑EPO group were significantly lower compared with the control group (P<0.05); however, the level of Hb in the CS‑TPP‑EPO group was higher compared with the other groups (P<0.05). The changes in Hb, BUN and Cr in the CS‑TPP‑EPO group were maintained for less than one week following the end of the treatment with CS‑TPP‑EPO nanoparticles. In conclusion, the CS‑TPP‑EPO nanoparticles had a lower toxicity compared with EPO and CS‑TPP treatment. Furthermore, CS‑TPP‑EPO may improve the therapeutic effect in the IgAN model. This suggests that CS‑TPP‑EPO nanoparticles may be a potential therapeutic drug for the treatment of patients with IgAN.

View Figures

View References

1	Weidemann A and Johnson RS: Nonrenal regulation of EPO synthesis. Kidney Int. 75:682–688. 2009. View Article : Google Scholar : PubMed/NCBI
2	Toba H, Nakashima K, Oshima Y, et al: Erythropoietin prevents vascular inflammation and oxidative stress in subtotal nephrectomized rat aorta beyond haematopoiesis. Clin Exp Pharmacol Physiol. 37:1139–1146. 2010. View Article : Google Scholar : PubMed/NCBI
3	Malgorzewicz S, Lichodziejewska-Niemierko M, Lizakowski S, Liberek T, Lysiak-Szydlowska W and Rutkowski B: Oxidative stress, inflammation and nutritional status during darbepoetin alpha treatment in peritoneal dialysis patients. Clin Nephrol. 73:210–215. 2010. View Article : Google Scholar
4	Ozawa T, Toba K, Suzuki H, et al; EPO/AMI-I Pilot Study Researchers. Single-dose intravenous administration of recombinant human erythropoietin is a promising treatment for patients with acute myocardial infarction - randomized controlled pilot trial of EPO/AMI-1 study. Circ J. 74:1415–1423. 2010. View Article : Google Scholar
5	Haljan G, Maitland A, Buchan A, et al: The Erythropoietin NeuroProtective Effect: Assessment in CABG Surgery (TENPEAKS): a randomized, double-blind, placebo controlled, proof-of-concept clinical trial. Stroke. 40:2769–2775. 2009. View Article : Google Scholar
6	Song YR, Lee T, You SJ, et al: Prevention of acute kidney injury by erythropoietin in patients undergoing coronary artery bypass grafting: a pilot study. Am J Nephrol. 30:253–260. 2009. View Article : Google Scholar : PubMed/NCBI
7	Fayed BE, Tawfik AF and Yassin AE: Novel erythropoietin-loaded nanoparticles with prolonged in vivo response. J Microencapsul. 29:650–656. 2012. View Article : Google Scholar : PubMed/NCBI
8	Chen H, Spagnoli F, Burris M, et al: Nanoerythropoietin is 10-times more effective than regular erythropoietin in neuroprotection in a neonatal rat model of hypoxia and ischemia. Stroke. 43:884–887. 2012. View Article : Google Scholar : PubMed/NCBI
9	Nagarwal RC, Singh PN, Kant S, Maiti P and Pandit JK: Chitosan nanoparticles of 5-fluorouracil for ophthalmic delivery: characterization, in-vitro and in-vivo study. Chem Pharm Bull (Tokyo). 59:272–278. 2011. View Article : Google Scholar : PubMed/NCBI
10	Mohammadpourdounighi N, Behfar A, Ezabadi A, Zolfagharian H and Heydari M: Preparation of chitosan nanoparticles containing Naja naja oxiana snake venom. Nanomedicine. 6:137–143. 2010. View Article : Google Scholar : PubMed/NCBI
11	Wang T, Hu Y, Zhang L, Jiang L, Chen Z and He N: Erythropoietin nanoparticles: therapy for cerebral ischemic injury and metabolize in kidney and liver. Nano Biomed Eng. 2:31–39. 2010. View Article : Google Scholar
12	Eren Z, Coban J, Ekinci ID, Kaspar C and Kantarci G: Evaluation of the effects of a high dose of erythropoietin-beta on early endotoxemia using a rat model. Adv Clin Exp Med. 21:321–329. 2012.PubMed/NCBI
13	Souza AC, Volpini RA, Shimizu MH, et al: Erythropoietin prevents sepsis-related acute kidney injury in rats by inhibiting NF-κB and upregulating endothelial nitric oxide synthase. Am J Physiol Renal Physiol. 302:F1045–F1054. 2012.PubMed/NCBI
14	Bulmer C, Margaritis A and Xenocostas A: Encapsulation and controlled release of recombinant human erythropoietin from chitosan-carrageenan nanoparticles. Curr Drug Deliv. 9:527–537. 2012. View Article : Google Scholar : PubMed/NCBI
15	Khachane P, Date AA and Nagarsenker MS: Eudragit EPO nanoparticles: application in improving therapeutic efficacy and reducing ulcerogenicity of meloxicam on oral administration. J Biomed Nanotechnol. 7:590–597. 2011. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of erythropoietin loading chitosan‑tripolyphosphate nanoparticles on an IgA nephropathy rat model

This article is mentioned in:

Abstract

Figure 1

Figure 2

Related Articles