Curcumin suppresses tumor necrosis factor‑α‑induced matrix metalloproteinase‑2 expression and activity in rat vascular smooth muscle cells via the NF‑κB pathway

Zhong,Yi; Yu,Wenyan; Feng,Jian; Fan,Zhongcai; Li, Jiafu

doi:10.3892/etm.2014.1647

Curcumin suppresses tumor necrosis factor‑α‑induced matrix metalloproteinase‑2 expression and activity in rat vascular smooth muscle cells via the NF‑κB pathway

Authors:
- Yi Zhong
- Wenyan Yu
- Jian Feng
- Zhongcai Fan
- Jiafu Li
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Affiliations: Department of Cardiology, The Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Department of Pathophysiology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: March 28, 2014 https://doi.org/10.3892/etm.2014.1647
Pages: 1653-1658

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Abstract

The aim of the present study was to investigate the inhibitory effect of curcumin on tumor necrosis factor (TNF)‑α‑induced cell migration and matrix metalloproteinase (MMP)‑2 expression and activity in rat vascular smooth muscle cells (VSMCs), in order to identify whether the effects are mediated by the nuclear factor (NF)‑κB signaling pathway. The VSMCs cells were pretreated with curcumin prior to stimulation with TNF‑α. Reverse transcription‑polymerase chain reaction and western blot analysis were used to determine the MMP‑2 mRNA and protein expression levels in TNF‑α‑stimulated VSMCs. Activity levels of MMP‑2 were measured using a gelatin zymography assay. Intracellular reactive oxygen species (ROS) generation was also analyzed. Curcumin was found to suppress the TNF‑α‑stimulated migration of VSMCs. In addition, curcumin inhibited the TNF‑α‑induced induction of MMP‑2 activity and expression. Curcumin also decreased ROS generation in TNF‑α‑stimulated VSMCs. Signal transduction experiments indicated that TNF‑α‑induced MMP‑2 expression in VSMCs was partly reversed with the application of an NF‑κB inhibitor (BAY11‑7082). In addition, western blot analysis revealed that curcumin reduced NF‑κB p65 protein expression in TNF‑α‑stimulated VSMCs at the concentration of 20 and 40 µM. Therefore, these observations indicated that curcumin suppressed TNF‑α‑stimulated VSMC migration and partially prevented TNF‑α‑induced MMP‑2 expression and activity in VSMCs via the NF‑κB pathway.

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