Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway

  • Authors:
    • Hongmin Shao
    • Jinghua Ma
    • Tianhua Guo
    • Rongrong Hu
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  • Published online on: May 26, 2014     https://doi.org/10.3892/etm.2014.1729
  • Pages: 505-508
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Abstract

Triptolide is a diterpene triepoxide compound extracted from the medicinal plant, Tripterygium wilfordii Hook F. The aim of the present study was to determine whether triptolide inhibits the proliferation of breast cancer cells and to further investigate the associated molecular mechanisms. The effects of triptolide on the cell viability of three breast cancer cell lines, specifically, highly metastatic MDA?MB?231, human epidermal growth factor receptor 2?positive BT?474 and estrogen receptor?positive MCF7 cells, were measured using 3?(4,5?dimethylthiazol?2?yl)?2,5?diphenyltetrazolium bromide and apoptosis assays. Western blot analysis was performed to investigate the expression levels of ??catenin in the control and triptolide?treated cells. The results demonstrated that triptolide treatment caused cell death in the three types of malignant cell lines. Treatment with 25 nM triptolide for 48 h exhibited marked inhibitory effects on the cell viability of the three types of cells, with greater effects observed in BT?474 cells compared with the other two cell types. When compared with the cells not treated with triptolide, 50 nM triptolide treatment resulted in apoptosis of MDA?MB?231, BT?474 and MCF7 cells with apoptotic rates of ~80%. Western blot analysis indicated that triptolide treatment of MDA?MB?231, BT?474 and MCF7 cells decreased the expression levels of ??catenin to 5?10% of the levels observed in the cells treated with dimethyl sulfoxide only. Therefore, the results of the present study indicate that triptolide induces the apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway.
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August-2014
Volume 8 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shao H, Ma J, Guo T and Hu R: Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway. Exp Ther Med 8: 505-508, 2014
APA
Shao, H., Ma, J., Guo, T., & Hu, R. (2014). Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway. Experimental and Therapeutic Medicine, 8, 505-508. https://doi.org/10.3892/etm.2014.1729
MLA
Shao, H., Ma, J., Guo, T., Hu, R."Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway". Experimental and Therapeutic Medicine 8.2 (2014): 505-508.
Chicago
Shao, H., Ma, J., Guo, T., Hu, R."Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/??catenin signaling pathway". Experimental and Therapeutic Medicine 8, no. 2 (2014): 505-508. https://doi.org/10.3892/etm.2014.1729