Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2014 Volume 8 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2014 Volume 8 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons

  • Authors:
    • Shuxing Xing
    • Cong Liu
    • Bing Xu
    • Jianchang Chen
    • Dongfeng Yin
    • Chunhao Zhang
  • View Affiliations / Copyright

    Affiliations: Second Department of Orthopedics, Ürümqi General Hospital, Lanzhou Command, Ürümqi, Xinjiang 830000, P.R. China
  • Pages: 628-634
    |
    Published online on: May 28, 2014
       https://doi.org/10.3892/etm.2014.1742
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t‑octyl‑phenoxypolyethoxyethanol (Triton‑X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri‑n‑butyl phosphate (TnBP), 1% Triton‑X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Triton‑X 100, were used on rabbit semitendinosus muscles and flexor digitorum tendons for 24 h to remove cells. Hematoxylin and eosin staining was applied for histological observation, while tension testing was used for biomechanical studies. The effects of the various decellularization methods on the histological structure and biomechanical properties of rabbit tendons were evaluated. A group of fresh tendons treated with phosphate‑buffered saline served as controls. The various decellularization methods resulted in different effects on the tendons. All the treatment groups exhibited a decrease in tendon biomechanical properties, but no statistically significant differences were observed among the experimental groups. The extensibility of the 1% TnBP‑treated group was found to be greater than that of the other groups; however, the difference was not statistically significant. Histologically, the 1% TnBP + 0.5% SDS treatment was shown to have the least impact on the rabbit tendon structure, with good decellularization and no clear cellular remnants observed. The 1% Triton‑X 100 + 0.5% SDS treatment had a pronounced effect on the tendon collagen structure and a number of collagen ruptures were observed. Overall, 1% TnBP + 0.5% SDS was found to be the most effective compared with the other treatments, as this treatment preserved the tendon collagen structure while completely removing the cells. Tendons treated with 1% TnBP + 0.5% SDS were histologically similar to normal tendon tissue and biomechanically similar to the tendons in the control group.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Potter HG, Jain SK, Ma Y, et al: Cartilage injury after acute, isolated anterior cruciate ligament tear: immediate and longitudinal effect with clinical/MRI follow-up. Am J Sports Med. 40:276–285. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Omae H, Sun YL, An KN, Amadio PC and Zhao C: Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells: an in vivo animal study. J Tissue Eng Regen Med. 6:238–244. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Leys T, Salmon L, Waller A, Linklater J and Pinczewski L: Clinical results and risk factors for reinjury 15 years after anterior cruciate ligament reconstruction: a prospective study of hamstring and patellar tendon grafts. Am J Sports Med. 40:595–605. 2012.PubMed/NCBI

4 

Harrison RD and Gratzer PF: Effect of extraction protocols and epidermal growth factor on the cellular repopulation of decellularized anterior cruciate ligament allografts. J Biomed Mater Res A. 75:841–854. 2005. View Article : Google Scholar

5 

Wilshaw SP, Kearney JN, Fisher J and Ingham E: Production of an acellular amniotic membrane matrix for use in tissue engineering. Tissue Eng. 12:2117–2129. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Kuo CK, Marturano JE and Tuan RS: Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs. Sports Med Arthrosc Rehabil Ther Technol. 2:202010. View Article : Google Scholar : PubMed/NCBI

7 

Longo UG, Lamberti A, Maffulli N and Denaro V: Tissue engineered biological augmentation for tendon healing: a systematic review. Br Med Bull. 98:31–59. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Chen X, Zou XH, Yin GL and Ouyang HW: Tendon tissue engineering with mesenchymal stem cells and biografts: an option for large tendon defects? Front Biosci (Schol Ed). 1:23–32. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Butler DL, Juncosa-Melvin N, Boivin GP, et al: Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation. J Orthop Res. 26:1–9. 2008. View Article : Google Scholar

10 

Karaoglu S, Fisher BM, Woo SL, et al: Use of a bioscaffold to improve healing of patellar tendon defect after graft harvest for ACL reconstruction: A study in rabbits. J Orthop Res. 26:255–263. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Worth DC, Hodivala-Dilke K, Robinson SD, et al: Alpha v beta3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migration. J Cell Biol. 189:369–383. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Tischer T, Vogt S, Aryee S, et al: Tissue engineering of the anterior cruciate ligament: a new method using acellularized tendon allografts and autologous fibroblasts. Arch Orthop Trauma Surg. 127:735–741. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Deeken CR, White AK, Bachman SL, et al: Method of preparing a decellularized porcine tendon using tributyl phosphate. J Biomed Mater Res B Appl Biomater. 96:199–206. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Lidén M, Sernert N, Rostgård-Christensen L, Kartus C and Ejerhed L: Osteoarthritic changes after anterior cruciate ligament reconstruction using bone-patellar tendon-bone or hamstring tendon autografts: a retrospective, 7-year radiographic and clinical follow-up study. Arthroscopy. 24:899–908. 2008.

15 

Butler DL, Juncosa-Melvin N, Boivin GP, et al: Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation. J Orthop Res. 26:1–9. 2008. View Article : Google Scholar

16 

Carey JL, Dunn WR, Dahm DL, Zeger SL and Spindler KP: A systematic review of anterior cruciate ligament reconstruction with autograft compared with allograft. J Bone Joint Surg Am. 91:2242–2250. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Gilbert TW, Sellaro TL and Badylak SF: Decellularization of tissues and organs. Biomaterials. 27:3675–3683. 2006.PubMed/NCBI

18 

Stone KR, Abdel-Motal UM, Walgenbach AW, Turek TJ and Galili U: Replacement of human anterior cruciate ligaments with pig ligaments: a model for anti-non-gal antibody response in long-term xenotransplantation. Transplantation. 83:211–219. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Tomford WW: Transmission of disease through transplantation of musculoskeletal allografts. J Bone Joint Surg Am. 77:1742–1754. 1995.PubMed/NCBI

20 

Malcarney HL, Bonar F and Murrell GA: Early inflammatory reaction after rotator cuff repair with a porcine small intestine submucosal implant: a report of 4 cases. Am J Sports Med. 33:907–911. 2005. View Article : Google Scholar

21 

Elder BD, Eleswarapu SV and Athanasiou KA: Extraction techniques for the decellularization of tissue engineered articular cartilage constructs. Biomaterials. 30:3749–3756. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Bhrany AD, Beckstead BL, Lang TC, Farwell DG, Giachelli CM and Ratner BD: Development of an esophagus acellular matrix tissue scaffold. Tissue Eng. 12:319–330. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Cartmell JS and Dunn MG: Effect of chemical treatments on tendon cellularity and mechanical properties. J Biomed Mater Res. 49:134–140. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Cartmell JS and Dunn MG: Development of cell-seeded patellar tendon allografts for anterior cruciate ligament reconstruction. Tissue Eng. 10:1065–1075. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Courtman DW, Errett BF and Wilson GJ: The role of crosslinking in modification of the immune response elicited against xenogenic vascular acellular matrices. J Biomed Mater Res. 55:576–586. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Woods T and Gratzer PF: Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament-bone graft. Biomaterials. 26:7339–7349. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xing S, Liu C, Xu B, Chen J, Yin D and Zhang C: Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons. Exp Ther Med 8: 628-634, 2014.
APA
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., & Zhang, C. (2014). Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons. Experimental and Therapeutic Medicine, 8, 628-634. https://doi.org/10.3892/etm.2014.1742
MLA
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., Zhang, C."Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons". Experimental and Therapeutic Medicine 8.2 (2014): 628-634.
Chicago
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., Zhang, C."Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons". Experimental and Therapeutic Medicine 8, no. 2 (2014): 628-634. https://doi.org/10.3892/etm.2014.1742
Copy and paste a formatted citation
x
Spandidos Publications style
Xing S, Liu C, Xu B, Chen J, Yin D and Zhang C: Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons. Exp Ther Med 8: 628-634, 2014.
APA
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., & Zhang, C. (2014). Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons. Experimental and Therapeutic Medicine, 8, 628-634. https://doi.org/10.3892/etm.2014.1742
MLA
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., Zhang, C."Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons". Experimental and Therapeutic Medicine 8.2 (2014): 628-634.
Chicago
Xing, S., Liu, C., Xu, B., Chen, J., Yin, D., Zhang, C."Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons". Experimental and Therapeutic Medicine 8, no. 2 (2014): 628-634. https://doi.org/10.3892/etm.2014.1742
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team