Dendrobium candidum Wall. ex Lindl. attenuates CCl4‑induced hepatic damage in imprinting control region mice

Li,Gui‑Jie; Sun,Peng; Wang,Qiang; Qian,Yu; Zhu,Kai; Zhao,Xin

doi:10.3892/etm.2014.1834

Dendrobium candidum Wall. ex Lindl. attenuates CCl4‑induced hepatic damage in imprinting control region mice

Authors:
- Gui‑Jie Li
- Peng Sun
- Qiang Wang
- Yu Qian
- Kai Zhu
- Xin Zhao
View Affiliations

Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
Published online on: July 8, 2014 https://doi.org/10.3892/etm.2014.1834
Pages: 1015-1021

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to determine the preventive effect of the traditional Chinese medicine, Dendrobium candidum Wall ex Lindl. (D. candidum), on CCl4‑induced hepatic damage in mice. The CCl4‑induced hepatic damage mice were treated with D. candidum, and the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), triglyceride (TG) and total cholesterol (TC) were determined. In addition, serum cytokine levels of interleukin (IL)‑6, IL‑12, tumor necrosis factor (TNF)‑α and interferon (IFN)‑γ were analyzed with kits, while liver tissues were analyzed using hematoxylin and eosin staining and reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the contents of D. candidum were determined by nuclear magnetic resonance (NMR). D. candidum was demonstrated to successfully prevent hepatic damage in mice. The serum levels of AST, ALT and LDH were significantly decreased when the mice were treated with 200 and 400 mg/kg D. candidum, as compared with the control mice (P<0.05). The lowest enzymatic activities were exhibited in the 400 mg/kg D. candidum group, which produced similar results to the positive control drug, silymarin. In addition, in the 400 mg/kg D. candidum group, the highest levels of TG and TC were observed among the treated groups. D. candidum‑treated groups also demonstrated reduced levels of the serum proinflammatory cytokines, IL‑6, IL‑12, TNF‑α and IFN‑γ. The sections of liver tissue examined during histopathology in the high concentration 400 mg/kg D. candidum group recovered well from CCl4 damage; however, the sections in the 200 mg/kg D. candidum group revealed necrosis to a more serious degree. RT‑PCR analysis was conducted on inflammation‑associated genes, including nuclear factor (NF)‑κB, IκB‑α, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)‑2, in the livers of the mice. The 400 mg/kg D. candidum group demonstrated significantly decreased mRNA expression levels of NF‑κB, iNOS and COX‑2, but an increased expression level of IκB‑α when compared with the CCl4‑treated control group. Furthermore, using NMR, 11 compounds were identified in the D. candidum leaf, whose functional contents may aid the preventive effect observed in the current study. Therefore, D. candidum may potentially contribute to the prevention of CCl4‑induced hepatic damage in vivo.

View Figures

View References

1	Wolf PL: Biochemical diagnosis of liver disease. Indian J Clin Biochem. 14:59–90. 1999. View Article : Google Scholar
2	Fried MW: Side effects of therapy of hepatitis C and their management. Hepatology. 36:S237–S244. 2002. View Article : Google Scholar : PubMed/NCBI
3	Wang Q, Sun P, Li G, Zhu K, Wang C and Zhao X: Inhibitory effects of Dendrobium candidum Wall ex Lindl. on azoxymethane- and dextran sulfate sodium-induced colon carcinogenesis in C57BL/6 mice. Oncol Lett. 7:493–498. 2014.
4	Xiao F, Zhang JZ and Tu YL: First report of Fusarium oxysporum causing wilt of Dendrobium candidum in Zhejiang Province, China. Plant Dis. 96:13772012.
5	Shao H, Zhang LQ, Li JM and Wei RC: Inhibitory effects of water extracts from four species of Dendrobiums on HelaS3 cells and HepG2 cells. J Anhui Agri Sci. 36:15968–15970. 2008.
6	Zhao X: Hawk tea (Litsea coreana Levl. var lanuginose) attenuates CCl(4)-induced hepatic damage in Sprague-Dawley rats. Exp Ther Med. 5:555–560. 2013.PubMed/NCBI
7	Zhao X, Kim SY and Park KY: Bamboo salt has in vitro anticancer activity in HCT-116 cells and exerts anti-metastatic effects in vivo. J Med Food. 16:9–19. 2013. View Article : Google Scholar : PubMed/NCBI
8	Ferenci P, Lockwood A, Mullen K, Tarter R, Weissenborn K and Blei AT: Hepatic encephalopathy - definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998. Hepatology. 35:716–721. 2002. View Article : Google Scholar
9	Li Y, Wang C, Wang F, Dong H, Guo S, Yang J and Xiao P: Chemical constituents of Dendrobium candidum. Zhongguo Zhong Yao Za Zhi. 35:1715–1719. 2010.(In Chinese).
10	Delić R and Stefanović M: Optimal laboratory panel for predicting preeclampsia. J Matern Fetal Neonatal Med. 23:96–102. 2010.PubMed/NCBI
11	Raju K, Anbuganapathi G, Gokulakrishnan V, Rajkapoor B, Jayakar B and Manian S: Effect of dried fruits of Solanum nigrum LINN against CCl₄-induced hepatic damage in rats. Biol Pharm Bull. 26:1618–1619. 2003.PubMed/NCBI
12	Halsted CH: Nutrition and alcoholic liver disease. Semin Liver Dis. 24:289–304. 2004. View Article : Google Scholar : PubMed/NCBI
13	Ghadir MR, Riahin AA, Havaspour A, Nooranipour M and Habibinejad AA: The relationship between lipid profile and severity of liver damage in cirrhotic patients. Hepat Mon. 10:285–288. 2010.PubMed/NCBI
14	Gratacós J, Collado A, Filella X, Sanmartí R, Cañete J, Llena J, Molina R, Ballesta A and Muñoz-Gómez J: Serum cytokines (IL-6, TNF-alpha, IL-1 beta and IFN-gamma) in ankylosing spondylitis: a close correlation between serum IL-6 and disease activity and severity. Br J Rheumatol. 33:927–931. 1994.PubMed/NCBI
15	Fox JG, Beck P, Dangler CA, Whary MT, Wang TC, Shi HN and Nagler-Anderson C: Concurrent enteric helminth infection modulates inflammation and gastric immune responses and reduces Helicobacter-induced gastric atrophy. Nat Med. 6:536–542. 2000. View Article : Google Scholar : PubMed/NCBI
16	D‘Elios MM, Manghetti M, De Carli M, Costa F, Baldari CT, Burroni D, Telford JL, Romagnani S and Del Prete G: T helper 1 effector cells specific for Helicobacter pylori in the gastric antrum of patients with peptic ulcer disease. J Immunol. 158:962–967. 1997.
17	Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB and Ruddle FH: Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15–p21. Genomics. 2:203–208. 1988.PubMed/NCBI
18	van der Poll T, Keogh CV, Guirao X, Buurman WA, Kopf M and Lowry SF: Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis. 176:439–444. 1997.PubMed/NCBI
19	Engel MA and Neurath MF: Anticancer properties of the IL-12 family - focus on colorectal cancer. Curr Med Chem. 17:3303–2208. 2010. View Article : Google Scholar : PubMed/NCBI
20	Gosselin D and Rivest S: Role of IL-1 and TNF in the brain: twenty years of progress on a Dr. Jekyll/Mr Hyde duality of the innate immune system. Brain Behav Immun. 21:281–289. 2007.PubMed/NCBI
21	Zhao X, Song JL, Kil JH and Park KY: Bamboo salt attenuates CCl₄-induced hepatic damage in Sprague-Dawley rats. Nutr Res Pract. 7:273–280. 2013.PubMed/NCBI
22	Girish C, Koner BC, Jayanthi S, Rao KR, Rajesh B and Pradhan SC: Hepatoprotective activity of six polyherbal formulations in CCl₄-induced liver toxicity in mice. Indian J Exp Biol. 47:257–263. 2009.PubMed/NCBI
23	Baeuerle PA: IkappaB-NF-kappaB structures: at the interface of inflammation control. Cell. 95:729–731. 1998. View Article : Google Scholar : PubMed/NCBI
24	Sánchez-Pérez I, Benitah SA, Martínez-Gomariz M, Lacal JC and Perona R: Cell stress and MEKK1-mediated c-Jun activation modulate NFkappaB activity and cell viability. Mol Biol Cell. 13:2933–2945. 2002.PubMed/NCBI
25	Hayden MS and Ghosh S: Signaling to NF-kappaB. Gene Dev. 18:2195–2224. 2004. View Article : Google Scholar : PubMed/NCBI
26	Kim SF, Huri DA and Snyder SH: Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science. 310:1966–1970. 2005. View Article : Google Scholar : PubMed/NCBI
27	Martín AR, Villegas I, La Casa C and de la Lastra CA: Resveratrol, a polyphenol found in grapes, suppresses oxidative damage and stimulates apoptosis during early colonic inflammation in rats. Biochem Pharmacol. 67:1399–1410. 2004.PubMed/NCBI
28	Zhou L and Pan JH: Role of adenosine and adenosine receptors in asthma. Int J Pediatr. 33:181–183. 2006.
29	Livingston M, Heaney LG and Ennis M: Adenosine, inflammation and asthma - a review. Inflamm Res. 53:171–178. 2004. View Article : Google Scholar
30	Evaldsson C, Rydén I and Uppugunduri S: Anti-inflammatory effects of exogenous uridine in an animal model of lung inflammation. Int Immunopharmacol. 7:1025–1032. 2007. View Article : Google Scholar : PubMed/NCBI
31	Liang Q, Liang ZS, Wang JR and Xu WH: Essential oil composition of Salvia miltiorrhiza flower. Food Chem. 113:592–594. 2009. View Article : Google Scholar