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Article

In vitro damage of Candida albicans biofilms by chitosan

  • Authors:
    • Yu Pu
    • Aibo Liu
    • Yuqiang Zheng
    • Bin Ye
  • View Affiliations / Copyright

    Affiliations: Department of Pathogenic Biology, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Medicine Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China
  • Pages: 929-934
    |
    Published online on: July 11, 2014
       https://doi.org/10.3892/etm.2014.1839
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Abstract

With the increasing usage of indwelling medical devices in clinical practice, the frequency of fungal infections has increased, such as that of Candida albicans (C. albicans). Biofilms, a protected niche for microorganisms, are resistant to a range of current antifungal agents. Chitosan is a polyatomic biopolymer with advantageous biocompatibility, biodegradation, nontoxicity and antibacterial properties. To investigate the inhibitory effect of chitosan on biofilms formed by C. albicans, cell viability, 2,3‑bis(2‑methoxy‑4‑nitro‑5‑sulfophenyl)‑2H‑tetrazolium‑5‑caboxanilide reduction, and morphological assays, including fluorescence microscopy and scanning electron microscopy (SEM), were employed. As assessed by cell viability assay, chitosan showed significant inhibitory effects on the planktonic cells and the biofilm of C. albicans in a dose‑dependent manner. Fluorescence microscopy and SEM assays confirmed that the chitosan‑treated group showed delayed C. albicans biofilm formation with defect morphological features, due to the inhibitory effects of the vast majority of fungal cell growth. In conclusion, C. albicans biofilms were compromised by the treatment with chitosan, providing an alternative therapeutic strategy against the fungal biofilms in the medical devices.
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1 

Hallahan AR, Shaw PJ, Rowell G, O’Connell A, Schell D and Gillis J: Improved outcomes of children with malignancy admitted to a pediatric intensive care unit. Crit Care Med. 28:3718–3721. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Booy R, Habibi P, Nadel S, et al: Reduction in case fatality rate from meningococcal disease associated with improved healthcare delivery. Arch Dis Child. 85:386–390. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Tenner PA, Dibrell H and Taylor RP: Improved survival with hospitalists in a pediatric intensive care unit. Crit Care Med. 31:847–852. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Chen Yan-jun WF: Study on pathogens causing lower respiratory tract infections and their drug resistance. Zhong Guo Bing Yuan Sheng Wu Xue Za Zhi. 8:561–563. 2013.(In Chinese).

5 

Adair CG, Gorman SP, Feron BM, et al: Implications of endotracheal tube biofilm for ventilator-associated pneumonia. Intensive Care Med. 25:1072–1076. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Crump JA and Collignon PJ: Intravascular catheter-associated infections. Eur J Clin Microbiol Infect Dis. 19:1–8. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Maki DG and Tambyah PA: Engineering out the risk for infection with urinary catheters. Emerg Infect Dis. 7:342–347. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Douglas LJ: Candida biofilms and their role in infection. Trends Microbiol. 11:30–36. 2003. View Article : Google Scholar

9 

Blankenship JR and Mitchell AP: How to build a biofilm: a fungal perspective. Curr Opin Microbiol. 9:588–594. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Chandra J, Mukherjee PK and Ghannoum MA: In vitro growth and analysis of Candida biofilms. Nat Protoc. 3:1909–1924. 2008. View Article : Google Scholar

11 

Carlson RP, Taffs R, Davison WM and Stewart PS: Anti-biofilm properties of chitosan-coated surfaces. J Biomater Sci Polym Ed. 19:1035–1046. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Alonso MJ and Sánchez A: The potential of chitosan in ocular drug delivery. J Pharm Pharmacol. 55:1451–1463. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Kulikov SN, Tiurin IuA, Fassakhov RS and Varlamov VP: Antibacterial and antimycotic activity of chitosan: mechanisms of action and role of the structure. Zh Mikrobiol Epidemiol Immunobiol. 5:91–97. 2009.(In Russian).

14 

Pasquantonio G, Greco C, Prenna M, et al: Antibacterial activity and anti-biofilm effect of chitosan against strains of Streptococcus mutans isolated in dental plaque. Int J Immunopathol Pharmacol. 21:993–997. 2008.PubMed/NCBI

15 

Meshulam T, Levitz SM, Christin L and Diamond RD: A simplified new assay for assessment of fungal cell damage with the tetrazolium dye, (2,3)-bis-(2-methoxy-4-nitro-5-sul- phenyl)-(2H)-tetrazolium-5-carboxanilide (XTT). J Infect Dis. 172:1153–1156. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Tsang PW, Bandara HM and Fong WP: Purpurin suppresses Candida albicans biofilm formation and hyphal development. PLoS One. 7:e508662012.PubMed/NCBI

17 

Albani JR and Plancke YD: Interaction between calcofluor white and carbohydrates of alpha 1-acid glycoprotein. Carbohydr Res. 314:169–175. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Wisplinghoff H, Ebbers J, Geurtz L, Stefanik D, Major Y, Edmond MB, Wenzel RP and Seifert H: Nosocomial bloodstream infections due to Candida spp. in the USA: species distribution, clinical features and antifungal susceptibilities. Int J Antimicrob Agents. 43:78–81. 2014.

19 

Georgopapadakou NH and Walsh TJ: Human mycoses: drugs and targets for emerging pathogens. Science. 264:371–373. 1994. View Article : Google Scholar : PubMed/NCBI

20 

Ramage G, VandeWalle K, Bachmann SP, Wickes BL and López-Ribot JL: In vitro pharmacodynamic properties of three antifungal agents against preformed Candida albicans biofilms determined by time-kill studies. Antimicrob Agents Chemother. 46:3634–3636. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Martinez LR, Mihu MR, Tar M, et al: Demonstration of antibiofilm and antifungal efficacy of chitosan against candidal biofilms, using an in vivo central venous catheter model. J Infect Dis. 201:1436–1440. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Hawser SP and Douglas LJ: Resistance of Candida albicans biofilms to antifungal agents in vitro. Antimicrob Agents Chemother. 39:2128–2131. 1995.

23 

Mukherjee PK, Chandra J, Kuhn DM and Ghannoum MA: Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols. Infect Immun. 71:4333–4340. 2003.PubMed/NCBI

24 

Rabea EI, Badawy ME, Stevens CV, Smagghe G and Steurbaut W: Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules. 4:1457–1465. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Sudarshan NR, Hoover DG and Knorr D: Antibacterial action of chitosan. Food Biotechnol. 6:257–272. 1992. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Pu Y, Liu A, Zheng Y and Ye B: In vitro damage of Candida albicans biofilms by chitosan. Exp Ther Med 8: 929-934, 2014.
APA
Pu, Y., Liu, A., Zheng, Y., & Ye, B. (2014). In vitro damage of Candida albicans biofilms by chitosan. Experimental and Therapeutic Medicine, 8, 929-934. https://doi.org/10.3892/etm.2014.1839
MLA
Pu, Y., Liu, A., Zheng, Y., Ye, B."In vitro damage of Candida albicans biofilms by chitosan". Experimental and Therapeutic Medicine 8.3 (2014): 929-934.
Chicago
Pu, Y., Liu, A., Zheng, Y., Ye, B."In vitro damage of Candida albicans biofilms by chitosan". Experimental and Therapeutic Medicine 8, no. 3 (2014): 929-934. https://doi.org/10.3892/etm.2014.1839
Copy and paste a formatted citation
x
Spandidos Publications style
Pu Y, Liu A, Zheng Y and Ye B: In vitro damage of Candida albicans biofilms by chitosan. Exp Ther Med 8: 929-934, 2014.
APA
Pu, Y., Liu, A., Zheng, Y., & Ye, B. (2014). In vitro damage of Candida albicans biofilms by chitosan. Experimental and Therapeutic Medicine, 8, 929-934. https://doi.org/10.3892/etm.2014.1839
MLA
Pu, Y., Liu, A., Zheng, Y., Ye, B."In vitro damage of Candida albicans biofilms by chitosan". Experimental and Therapeutic Medicine 8.3 (2014): 929-934.
Chicago
Pu, Y., Liu, A., Zheng, Y., Ye, B."In vitro damage of Candida albicans biofilms by chitosan". Experimental and Therapeutic Medicine 8, no. 3 (2014): 929-934. https://doi.org/10.3892/etm.2014.1839
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