Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro

  • Authors:
    • Ren Feng Shan
    • Yun Fei Zhou
    • Ai Fen Peng
    • Zhi Gang Jie
  • View Affiliations

  • Published online on: July 14, 2014     https://doi.org/10.3892/etm.2014.1844
  • Pages: 1005-1009
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Abstract

In the present study, the effect of Aurora‑B inhibition on HepG2 cell invasion and migration in vitro was investigated. A recombinant plasmid targeting the Aurora‑B gene (MiR‑Aurora‑B) was used to inhibit Aurora‑B expression in HepG2 cells. Cell migration and invasion were investigated using Transwell migration and invasion assays. The results demonstrated that cell invasion and migration were suppressed by inhibiting Aurora‑B. In addition, the effect of Aurora‑B inhibition on the activity of the phosphoinositide 3‑kinase (PI3K)/Akt/nuclear factor (NF)‑κB signaling pathway was investigated by analyzing the protein expression levels of phosphorylated (p)‑Akt, Akt, NF‑κB p65, matrix metalloproteinase (MMP)‑2 and MMP‑9 using western blot analysis. The results demonstrated that the protein expression levels of p‑Akt, NF‑κB p65, MMP‑2 and MMP‑9 were reduced significantly by inhibiting Aurora‑B. Therefore, inhibition of Aurora‑B was shown to suppress hepatocellular carcinoma cell migration and invasion by decreasing the activity of the PI3K/Akt/NF‑κB signaling pathway in vitro.
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September-2014
Volume 8 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shan RF, Zhou YF, Peng AF and Jie ZG: Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro. Exp Ther Med 8: 1005-1009, 2014
APA
Shan, R.F., Zhou, Y.F., Peng, A.F., & Jie, Z.G. (2014). Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro. Experimental and Therapeutic Medicine, 8, 1005-1009. https://doi.org/10.3892/etm.2014.1844
MLA
Shan, R. F., Zhou, Y. F., Peng, A. F., Jie, Z. G."Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro". Experimental and Therapeutic Medicine 8.3 (2014): 1005-1009.
Chicago
Shan, R. F., Zhou, Y. F., Peng, A. F., Jie, Z. G."Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro". Experimental and Therapeutic Medicine 8, no. 3 (2014): 1005-1009. https://doi.org/10.3892/etm.2014.1844