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Article

Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice

  • Authors:
    • Xian‑Long Zhou
    • Yu‑Lu Shi
    • Xiong Li
  • View Affiliations / Copyright

    Affiliations: Emergency Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China, Medical College of Wuhan University, Wuhan, Hubei 430072, P.R. China, Department of Ultrasound, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 1159-1163
    |
    Published online on: August 4, 2014
       https://doi.org/10.3892/etm.2014.1877
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Abstract

The aim of the present study was to investigate the effect of the ultrasound‑targeted microbubble destruction mediated (UTMD) herpes simplex virus‑thymidine kinase (HSV‑TK) and ganciclovir (GCV) system on ovarian cancer (OC). This study was conducted between June and December 2012 in the Animal Biosafety Level III Laboratory of Wuhan University. Mice with OC were randomly divided into four groups: i) HSV‑TK plus microbubbles (MBs) plus ultrasound (US) (n=15); ii) HSV‑TK plus US (n=15); iii) HSV‑TK (n=15); and iv) phosphate‑buffered saline (n=15). The inhibitory effect and survival time in the experimental groups were compared with those in the control group. The TK protein expression was detected by western blot analysis. Tumor cell apoptosis was detected by terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling and caspase‑3 activity analysis. The data showed that the efficiency of HSV‑TK gene transfection and the tumor inhibitory effects were significantly increased in the HSV‑TK plus MBs plus US group compared with those in the control group (P<0.01). UTMD‑mediated HSV‑TK treatment has also improved the rat survival rate (P<0.01). In conclusion, UTMD can effectively transfect the HSV‑TK gene into target tissues and exert a significant inhibitory effect on OC in mice.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou XL, Shi YL and Li X: Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice. Exp Ther Med 8: 1159-1163, 2014.
APA
Zhou, X., Shi, Y., & Li, X. (2014). Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice. Experimental and Therapeutic Medicine, 8, 1159-1163. https://doi.org/10.3892/etm.2014.1877
MLA
Zhou, X., Shi, Y., Li, X."Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice". Experimental and Therapeutic Medicine 8.4 (2014): 1159-1163.
Chicago
Zhou, X., Shi, Y., Li, X."Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1159-1163. https://doi.org/10.3892/etm.2014.1877
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou XL, Shi YL and Li X: Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice. Exp Ther Med 8: 1159-1163, 2014.
APA
Zhou, X., Shi, Y., & Li, X. (2014). Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice. Experimental and Therapeutic Medicine, 8, 1159-1163. https://doi.org/10.3892/etm.2014.1877
MLA
Zhou, X., Shi, Y., Li, X."Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice". Experimental and Therapeutic Medicine 8.4 (2014): 1159-1163.
Chicago
Zhou, X., Shi, Y., Li, X."Inhibitory effects of the ultrasound‑targeted microbubble destruction‑mediated herpes simplex virus‑thymidine kinase/ganciclovir system on ovarian cancer in mice". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1159-1163. https://doi.org/10.3892/etm.2014.1877
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