Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement

Guo,Jinhui; Chang,Li; Zhang,Xin; Pei,Sujuan; Yu,Meishuang; Gao,Jianlian

doi:10.3892/etm.2014.1885

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Article

Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement

Authors:
- Jinhui Guo
- Li Chang
- Xin Zhang
- Sujuan Pei
- Meishuang Yu
- Jianlian Gao
View Affiliations / Copyright

Affiliations: Department of Pharmaceutics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
Pages: 1271-1274
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Published online on: August 6, 2014

https://doi.org/10.3892/etm.2014.1885
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Abstract

The aim of the present study was to investigate the effect of ginsenoside compound K on β‑amyloid (Aβ) peptide clearance in primary astrocytes. Aβ degradation in primary astrocytes was determined using an intracellular Aβ clearance assay. Aggregated LC3 in astrocyte cells, which is a marker for the level of autophagy, was detected using laser scanning confocal microscope. The effect of compound K on the mammalian target of rapamycin (mTOR)/autophagy pathway was determined using western blot analysis, and an enzyme‑linked immunosorbent assay was used for Aβ detection. The results demonstrated that compound K promoted the clearance of Aβ and enhanced autophagy in primary astrocytes. In addition, it was found that phosphorylation of mTOR was inhibited by compound K, which may have contributed to the enhanced autophagy. In conclusion, compound K promotes Aβ clearance by enhancing autophagy via the mTOR signaling pathway in primary astrocytes.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement

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