Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement

  • Authors:
    • Jinhui Guo
    • Li Chang
    • Xin Zhang
    • Sujuan Pei
    • Meishuang Yu
    • Jianlian Gao
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  • Published online on: August 6, 2014     https://doi.org/10.3892/etm.2014.1885
  • Pages: 1271-1274
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Abstract

The aim of the present study was to investigate the effect of ginsenoside compound K on β‑amyloid (Aβ) peptide clearance in primary astrocytes. Aβ degradation in primary astrocytes was determined using an intracellular Aβ clearance assay. Aggregated LC3 in astrocyte cells, which is a marker for the level of autophagy, was detected using laser scanning confocal microscope. The effect of compound K on the mammalian target of rapamycin (mTOR)/autophagy pathway was determined using western blot analysis, and an enzyme‑linked immunosorbent assay was used for Aβ detection. The results demonstrated that compound K promoted the clearance of Aβ and enhanced autophagy in primary astrocytes. In addition, it was found that phosphorylation of mTOR was inhibited by compound K, which may have contributed to the enhanced autophagy. In conclusion, compound K promotes Aβ clearance by enhancing autophagy via the mTOR signaling pathway in primary astrocytes.
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October 2014
Volume 8 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Guo J, Chang L, Zhang X, Pei S, Yu M and Gao J: Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement. Exp Ther Med 8: 1271-1274, 2014
APA
Guo, J., Chang, L., Zhang, X., Pei, S., Yu, M., & Gao, J. (2014). Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement. Experimental and Therapeutic Medicine, 8, 1271-1274. https://doi.org/10.3892/etm.2014.1885
MLA
Guo, J., Chang, L., Zhang, X., Pei, S., Yu, M., Gao, J."Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement". Experimental and Therapeutic Medicine 8.4 (2014): 1271-1274.
Chicago
Guo, J., Chang, L., Zhang, X., Pei, S., Yu, M., Gao, J."Ginsenoside compound K promotes β‑amyloid peptide clearance in primary astrocytes via autophagy enhancement". Experimental and Therapeutic Medicine 8, no. 4 (2014): 1271-1274. https://doi.org/10.3892/etm.2014.1885