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Article

Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis

  • Authors:
    • Amal Hassanin
    • Hala Abdel Malek
    • Dalia Saleh
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt, Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
  • Pages: 1551-1558
    |
    Published online on: September 15, 2014
       https://doi.org/10.3892/etm.2014.1963
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Abstract

Nonalcoholic fatty liver disease (NAFLD) is considered to be a hepatic manifestation of metabolic syndrome, and has been etiologically associated with insulin resistance (IR). The histopathology of NAFLD ranges between simple steatosis and nonalcoholic steatohepatitis (NASH), with or without fibrosis. The aim of the present study was to examine the effect of heparin on steatohepatitis and hepatic‑induced nitric oxide synthase (iNOS) expression in mice. Male mice were divided into four groups, which included the normal basal diet (control), high fat (HF) diet, HF diet + heparin (treatment group) and heparin control groups. After eight weeks from the initiation of the experiment, blood was collected and livers were harvested for biochemical analysis and histological studies. Serum levels of aspartate aminotransferase, alanine aminotransferase, hepatic triglyceride (TG) and hydroxyproline, as well as the IR, superoxide anion generation and mRNA expression of the hepatic iNOS enzyme were evaluated. Liver specimens were processed for histopathological and immunohistopathological evaluation. Heparin administration decreased the levels of the liver enzymes, IR, superoxide generation, hepatic TG, hydroxyproline and iNOS expression when compared with the HF diet group. These changes were associated with an improvement in inflammation and fibrosis observed via histopathological examination. Therefore, heparin treatment attenuates hepatic injury in steatohepatitis.
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Copy and paste a formatted citation
Spandidos Publications style
Hassanin A, Malek HA and Saleh D: Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis. Exp Ther Med 8: 1551-1558, 2014.
APA
Hassanin, A., Malek, H.A., & Saleh, D. (2014). Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis. Experimental and Therapeutic Medicine, 8, 1551-1558. https://doi.org/10.3892/etm.2014.1963
MLA
Hassanin, A., Malek, H. A., Saleh, D."Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis". Experimental and Therapeutic Medicine 8.5 (2014): 1551-1558.
Chicago
Hassanin, A., Malek, H. A., Saleh, D."Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1551-1558. https://doi.org/10.3892/etm.2014.1963
Copy and paste a formatted citation
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Spandidos Publications style
Hassanin A, Malek HA and Saleh D: Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis. Exp Ther Med 8: 1551-1558, 2014.
APA
Hassanin, A., Malek, H.A., & Saleh, D. (2014). Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis. Experimental and Therapeutic Medicine, 8, 1551-1558. https://doi.org/10.3892/etm.2014.1963
MLA
Hassanin, A., Malek, H. A., Saleh, D."Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis". Experimental and Therapeutic Medicine 8.5 (2014): 1551-1558.
Chicago
Hassanin, A., Malek, H. A., Saleh, D."Heparin modulation on hepatic nitric oxide synthase in experimental steatohepatitis". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1551-1558. https://doi.org/10.3892/etm.2014.1963
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