Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems

  • Authors:
    • Ju Liu
    • Urban Deutsch
    • Iris Fung
    • Corrinne G. Lobe
  • View Affiliations / Copyright

    Affiliations: Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Theodor‑Kocher‑Institute, University of Berne, Berne CH‑3012, Switzerland, Molecular and Cellular Biology Division, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada
  • Pages: 1351-1356
    |
    Published online on: September 15, 2014
       https://doi.org/10.3892/etm.2014.1965
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the tetracycline‑off and Cre/loxP systems were combined to gain temporal and spatial control of transgene expression. Mice were generated that carried three transgenes: Tie2‑tTA, tet‑O‑Cre and either the ZEG or ZAP reporter. Tie2‑tTA directs expression of tetracycline‑controlled transactivator (tTA) in endothelial and hematopoietic cells under the control of the Tie2 promoter. Tet‑O‑Cre produces Cre recombinase from a minimal promoter containing the tet‑operator (tetO). ZEG or ZAP contains a strong promoter and a loxP‑flanked stop sequence, followed by an enhanced green fluorescence protein (EGFP) or human placental alkaline phosphatase (hPLAP) reporter. In the presence of tetracycline, the tTA transactivator produced by Tie‑2‑tTA is disabled and Cre is not expressed. In the absence of tetracycline, the tTA binds tet‑O‑Cre to drive the expression of Cre, which recombines the loxP sites of the ZEG or ZAP transgene and results in reporter gene expression. In the present study, the expression of the ZEG or ZAP reporter genes in embryos and adult animals with and without tetracycline treatment was examined. In the presence of tetracycline, no reporter gene expression was observed. When tetracycline was withdrawn, Cre excision was activated and the reporter genes were detected in endothelial and hematopoietic cells. These results demonstrate that this system may be used to bypass embryonic lethality and access adult phenotypes.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Rossant J and Howard L: Signaling pathways in vascular development. Annu Rev Cell Dev Biol. 18:541–573. 2002. View Article : Google Scholar

2 

Alva JA and Iruela-Arispe ML: Notch signaling in vascular morphogenesis. Curr Opin Hematol. 11:278–283. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Limbourg FP, Takeshita K, Radtke F, et al: Essential role of endothelial Notch1 in angiogenesis. Circulation. 111:1826–1832. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Krebs LT, Shutter JR, Tanigaki K, et al: Haploinsufficient lethality and formation of arteriovenous malformations in Notch pathway mutants. Genes Dev. 18:2469–2473. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Duarte A, Hirashima M, Benedito R, et al: Dosage-sensitive requirement for mouse Dll4 in artery development. Genes Dev. 18:2474–2478. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Krebs LT, Xue Y, Norton CR, et al: Notch signaling is essential for vascular morphogenesis in mice. Genes Dev. 14:1343–1352. 2000.PubMed/NCBI

7 

Uyttendaele H, Ho J, Rossant J and Kitajewski J: Vascular patterning defects associated with expression of activated Notch4 in embryonic endothelium. Proc Natl Acad Sci USA. 98:5643–5648. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Baim SB, Labow MA, Levine AJ and Shenk T: A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl β-D-thiogalactopyranoside. Proc Natl Acad Sci USA. 88:5072–5076. 1991.PubMed/NCBI

9 

Wyborski DL and Short JM: Analysis of inducers of the E.coli lac repressor system in mammalian cells and whole animals. Nucleic Acids Res. 19:4647–4653. 1991.PubMed/NCBI

10 

Plück A: Conditional mutagenesis in mice: the Cre/loxP recombination system. Int J Exp Pathol. 77:269–278. 1996.PubMed/NCBI

11 

Kos CH: Cre/loxP system for generating tissue-specific knockout mouse models. Nutr Rev. 62:243–246. 2004.PubMed/NCBI

12 

Lewandoski M: Conditional control of gene expression in the mouse. Nat Rev Genet. 2:743–755. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Lobe CG, Koop KE, Kreppner W, et al: Z/AP, a double reporter for Cre-mediated recombination. Dev Biol. 208:281–292. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Liu J and Lobe CG: Cre-conditional expression of constitutively active Notch1 in transgenic mice. Genesis. 45:259–265. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Gossen M and Bujard H: Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA. 89:5547–5551. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Gossen M, Freundlieb S, Bender G, et al: Transcriptional activation by tetracyclines in mammalian cells. Science. 268:1766–1769. 1995. View Article : Google Scholar : PubMed/NCBI

17 

St-Onge L, Furth PA and Gruss P: Temporal control of the Cre recombinase in transgenic mice by a tetracycline responsive promoter. Nucleic Acids Res. 24:3875–3877. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Novak A, Guo C, Yang W, Nagy A and Lobe CG: Z/EG, a double reporter transgenic mouse line that expresses enhanced green fluorescent protein upon Cre-mediated recombination. genesis. 28:147–155. 2000. View Article : Google Scholar

19 

Schlaeger TM, Bartunkova S, Lawitts JA, et al: Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Proc Natl Acad Sci USA. 94:3058–3063. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Guo C, Yang W and Lobe CG: A Cre recombinase transgene with mosaic, widespread tamoxifen-inducible action. Genesis. 32:8–18. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Takakura N, Huang XL, Naruse T, et al: Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis. Immunity. 9:677–686. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Kisanuki YY, Hammer RE, Miyazaki J, et al: Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Dev Biol. 230:230–242. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Sarao R and Dumont DJ: Conditional transgene expression in endothelial cells. Transgenic Res. 7:421–427. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Rammos C, Hendgen-Cotta UB, Deenen R, et al: Age-related vascular gene expression profiling in mice. Mech Ageing Dev. 135:15–23. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Stan RV, Tse D, Deharvengt SJ, et al: The diaphragms of fenestrated endothelia: gatekeepers of vascular permeability and blood composition. Dev Cell. 23:1203–1218. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Liu Y, Carson-Walter EB, Cooper A, et al: Vascular gene expression patterns are conserved in primary and metastatic brain tumors. J Neurooncol. 99:13–24. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Steinman RA: Cell cycle regulators and hematopoiesis. Oncogene. 21:3403–3413. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Smith C: Hematopoietic stem cells and hematopoiesis. Cancer Control. 10:9–16. 2003.PubMed/NCBI

29 

Tang J, Shao W, Dorak MT, et al: Positive and negative associations of human leukocyte antigen variants with the onset and prognosis of adult glioblastoma multiforme. Cancer Epidemiol Biomarkers Prev. 14:2040–2044. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Dorak MT, Burnett AK and Worwood M: HFE gene mutations in susceptibility to childhood leukemia: HuGE review. Genet Med. 7:159–168. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Brumpt C, Delabesse E, Beldjord K, et al: The incidence of clonal T-cell receptor rearrangements in B-cell precursor acute lymphoblastic leukemia varies with age and genotype. Blood. 96:2254–2261. 2000.PubMed/NCBI

32 

Belteki G, Haigh J, Kabacs N, et al: Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res. 33:e512005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu J, Deutsch U, Fung I and Lobe CG: Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems. Exp Ther Med 8: 1351-1356, 2014.
APA
Liu, J., Deutsch, U., Fung, I., & Lobe, C.G. (2014). Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems. Experimental and Therapeutic Medicine, 8, 1351-1356. https://doi.org/10.3892/etm.2014.1965
MLA
Liu, J., Deutsch, U., Fung, I., Lobe, C. G."Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems". Experimental and Therapeutic Medicine 8.5 (2014): 1351-1356.
Chicago
Liu, J., Deutsch, U., Fung, I., Lobe, C. G."Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1351-1356. https://doi.org/10.3892/etm.2014.1965
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Deutsch U, Fung I and Lobe CG: Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems. Exp Ther Med 8: 1351-1356, 2014.
APA
Liu, J., Deutsch, U., Fung, I., & Lobe, C.G. (2014). Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems. Experimental and Therapeutic Medicine, 8, 1351-1356. https://doi.org/10.3892/etm.2014.1965
MLA
Liu, J., Deutsch, U., Fung, I., Lobe, C. G."Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems". Experimental and Therapeutic Medicine 8.5 (2014): 1351-1356.
Chicago
Liu, J., Deutsch, U., Fung, I., Lobe, C. G."Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline‑off systems". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1351-1356. https://doi.org/10.3892/etm.2014.1965
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team