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Article

Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells

  • Authors:
    • Wei‑Guo Hu
    • Qi‑Ping Lu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Wuhan General Hospital of Guangzhou Military Command, Wuhan, Hubei 430070, P.R. China
  • Pages: 1438-1442
    |
    Published online on: September 18, 2014
       https://doi.org/10.3892/etm.2014.1979
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Abstract

In the present study the effect of reactive oxygen species on the morphological changes of pancreatic epithelial cells in a three‑dimensional culture system was investigated. In addition, the expression of signaling molecules during this process was determined. Matrigel™ was used to construct a three‑dimensional culture model of pancreatic epithelial and cancer cells. The cultured cells were stimulated with 1 or 200 µmol/l H2O2 (a typical reactive oxygen species), and the morphological changes were then evaluated after 15 min, 1 h and 4 h. The cytoskeleton of the cells was observed using laser scanning confocal microscopy with immunofluorescence staining. In addition, the nuclear content of nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) was detected using ELISA. The results demonstrated that treatment with 200 µmol/l H2O2 induced cell contraction after 15 min, and cell morphology recovered after 1 h; however, cell size was reduced after 4 h. Consequently, intracellular actin and microtubules were rapidly lost following H2O2 treatment, and the cytoskeleton became indistinct and eventually disintegrated after 4 h. Similar observations were noted for the normal pancreatic epithelial and cancer cells. By contrast, treatment with 1 µmol/l H2O2 did not affect the morphology and cytoskeleton of pancreatic epithelial cells. In addition, 200 µmol/l H2O2 treatment increased the activity of NF‑κB gradually, while 1 µmol/l H2O2 treatment was found to have little impact on the activity of NF‑κB. Therefore, it was demonstrated that oxidative stress can induce the early onset of reversible cell contraction and cytoskeleton depolarization in pancreatic epithelial cells, and can increase NF‑κB expression.
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1 

Schulz HU, Niederau C, Klonowski-Stumpe H, et al: Oxidative stress in acute pancreatitis. Hepatogastroenterology. 46:2736–2750. 1999.PubMed/NCBI

2 

Verlaan M, Roelofs HM, van-Schaik A, et al: Assessment of oxidative stress in chronic pancreatitis patients. World J Gastroenterol. 12:5705–5710. 2006.PubMed/NCBI

3 

Li J, Lee JM and Johnson JA: Microarray analysis reveals an antioxidant responsive element-driven gene set involved in conferring protection from an oxidative stress-induced apoptosis in IMR-32 cells. J Biol Chem. 277:388–394. 2002. View Article : Google Scholar

4 

Dandrea T, Hellmold H, Jonsson C, et al: The transcriptosomal response of human A549 lung cells to a hydrogen peroxide-generating system: relationship to DNA damage, cell cycle arrest, and caspase activation. Free Radic Biol Med. 36:881–896. 2004. View Article : Google Scholar

5 

Katsube T, Tsuji H and Onoda M: Nitric oxide attenuates hydrogen peroxide-induced barrier disruption and protein tyrosine phosphorylation in monolayers of intestinal epithelial cell. Biochim Biophys Acta. 1773:794–803. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Tudek B, Winczura A, Janik J, et al: Involvement of oxidatively damaged DNA and repair in cancer development and aging. Am J Transl Res. 2:254–284. 2010.PubMed/NCBI

7 

Jałoszyński P, Jaruga P, Oliński R, et al: Oxidative DNA base modifications and polycyclic aromatic hydrocarbon DNA adducts in squamous cell carcinoma of larynx. Free Radic Res. 37:231–240. 2003.PubMed/NCBI

8 

Lim JW, Song JY, Seo JY, et al: Role of pancreatitis-associated protein 1 on oxidative stress-induced cell death of pancreatic acinar cells. Ann NY Acad Sci. 1171:545–548. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Weber H, Hühns S, Jonas L, et al: Hydrogen peroxide-induced activation of defense mechanisms against oxidative stress in rat pancreatic acinar AR42J. Free Radic Biol Med. 42:830–841. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Etemad B and Whitcomb DC: Chronic pancreatitis: diagnosis, classification, and new genetic developments. Gastroenterology. 120:682–707. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Weber H, Hühns S, Lüthen F and Joanas L: Calpain-mediated breakdown of cytoskeletal proteins contributes to cholecystokinin-induced damage of rat pancreatic acini. Int J Exp Pathol. 90:387–399. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Banan A, Choudhary S, Zhang Y, et al: Oxidant-induced intestinal barrier disruption and its prevention by growth factors in a human colonic cell line: role of the microtubule cytoskeleton. Free Radic Biol Med. 28:727–738. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Fatokun AA, Stone TW and Smith RA: Hydrogen peroxide-induced oxidative stress in MC3T3-E1 cells: The effects of glutamate and protection by purines. Bone. 39:542–551. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Valen G, Sondén A, Vaage J, et al: Hydrogen peroxide induces endothelial cell atypia and cytoskeleton depolymerization. Free Radic Biol Med. 26:1480–1488. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Bijlsma MF, Borensztajn KS, Roelink H, et al: Sonic hedgehog induces transcription-independent cytoskeletal rearrangement and migration regulated by arachidonate metabolites. Cell Signal. 19:2596–2604. 2007. View Article : Google Scholar

16 

Banan A, Keshavarzian A, Zhang L, et al: NF-kappaB activation as a key mechanism in ethanol-induced disruption of the F-actin cytoskeleton and monolayer barrier integrity in intestinal epithelium. Alcohol. 41:447–460. 2007. View Article : Google Scholar

17 

Nakashima H, Nakamura M, Yamaguchi H, et al: Nuclear factor-kappaB contributes to hedgehog signaling pathway activation through sonic hedgehog induction in pancreatic cancer. Cancer Res. 66:7041–7049. 2006. View Article : Google Scholar

18 

Arjona-Sánchez A, Ruiz-Rabelo J, Perea MD, et al: Effects of capecitabine and celecoxib in experimental pancreatic cancer. Pancreatology. 10:641–647. 2010.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hu WG and Lu QP: Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells. Exp Ther Med 8: 1438-1442, 2014.
APA
Hu, W., & Lu, Q. (2014). Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells. Experimental and Therapeutic Medicine, 8, 1438-1442. https://doi.org/10.3892/etm.2014.1979
MLA
Hu, W., Lu, Q."Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells". Experimental and Therapeutic Medicine 8.5 (2014): 1438-1442.
Chicago
Hu, W., Lu, Q."Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1438-1442. https://doi.org/10.3892/etm.2014.1979
Copy and paste a formatted citation
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Spandidos Publications style
Hu WG and Lu QP: Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells. Exp Ther Med 8: 1438-1442, 2014.
APA
Hu, W., & Lu, Q. (2014). Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells. Experimental and Therapeutic Medicine, 8, 1438-1442. https://doi.org/10.3892/etm.2014.1979
MLA
Hu, W., Lu, Q."Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells". Experimental and Therapeutic Medicine 8.5 (2014): 1438-1442.
Chicago
Hu, W., Lu, Q."Impact of oxidative stress on the cytoskeleton of pancreatic epithelial cells". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1438-1442. https://doi.org/10.3892/etm.2014.1979
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