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Article

Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells

  • Authors:
    • Haiyan Wang
    • Luping Zhang
    • Shaoyan Zhang
    • Yannian Li
  • View Affiliations / Copyright

    Affiliations: Department of Transfusion, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong 266033, P.R. China, Clinical Laboratory, Qingdao Hiser Hospital, Qingdao, Shandong 266033, P.R. China, Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, Shandong 266033, P.R. China
  • Pages: 120-124
    |
    Published online on: November 11, 2014
       https://doi.org/10.3892/etm.2014.2059
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Abstract

This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) secreted by MCF‑7 breast cancer cells on the differentiation, maturation and function of dendritic cells (DCs). Small interfering RNAs (siRNAs) directed against the VEGF gene were designed and transfected into MCF‑7 breast cancer cells at an optimal concentration (100 nmol/l) using cationic liposome transfection reagent, whereas the control group was transfected with only transfection reagent. Western blot analysis and ELISA were used to determine VEGF protein expression and VEGF concentration, respectively. Mononuclear cells were cultured with the culture supernatants from primary MCF‑7 cells (control group) and siRNA‑treated MCF‑7 cells (siRNA group). The DC phenotypes, including CD1a, CD80, CD83, CD86 and HLA‑DR, were evaluated by flow cytometry. The MTT assay was used to assess the cytotoxicity of DC‑mediated tumor‑specific cytotoxic T lymphocytes (CTLs) against MCF‑7 cells in the two different culture supernatants. The VEGF‑targeted constructed siRNA inhibited VEGF expression in MCF‑7 cells. Cultivation with the culture supernatants from MCF‑7 cells treated with siRNA affected DC morphology. DCs in the siRNA group exhibited a significantly higher expression of CD86, CD80, CD83 and HLA‑DR compared to the cells in the control group, whereas the expression of CD1a in the siRNA group was significantly lower compared to that in the control group. The cytotoxic activity of CTLs mediated by DCs was significantly altered by siRNA transfection. These results indicated that VEGF may play a significant role in tumor development, progression and immunosuppression.
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1 

Steinman RM and Cohn ZA: Identification of a novel cell type in peripheral lymphoid organs of mice. I Morphology, quantitation, tissue distribution. J Exp Med. 137:1142–1162. 1973. View Article : Google Scholar : PubMed/NCBI

2 

Cella M, Sallusto F and Lanzavecchia A: Origin, maturation and antigen presenting function of dendritic cells. Curr Opin Immunol. 9:10–16. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Hart DN: Dendritic cells: unique leukocyte populations which control the primary immune response. Blood. 90:3245–3287. 1997.PubMed/NCBI

4 

Steinman RM: The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 9:271–296. 1991. View Article : Google Scholar : PubMed/NCBI

5 

Banchereau J and Steinman RM: Dendritic cells and the control of immunity. Nature. 392:245–252. 1998. View Article : Google Scholar : PubMed/NCBI

6 

Baird JR, Fox BA, Sanders KL, et al: Avirulent Toxoplasma gondii generates therapeutic antitumor immunity by reversing immunosuppression in the ovarian cancer microenvironment. Cancer Res. 73:3842–3851. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Schuler G and Steinman RM: Dendritic cells as adjuvants for immune-mediated resistance to tumors. J Exp Med. 186:1183–1187. 1997. View Article : Google Scholar

8 

Gabrilovich D, Ciernik F and Carbone DP: Dendritic cells in anti-tumor immune responses. I Defective antigen presentation in tumor-bearinghosts. Cell Immunol. 170:101–110. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Chaux P, Moutet M, Faivre J, Martin F and Martin M: Inflammatory cells infiltrating human colorectal carcinomas express HLA class II but not B7-1 and B7-2 costimulatory molecules of the T-cell activation. Lab Invest. 74:975–983. 1996.PubMed/NCBI

10 

Chaux P, Favre N, Martin M and Martin F: Tumor-infiltrating dendritic cells are defective in their antigen-presenting function and inducible B7 expression in rats. Int J Cancer. 72:619–624. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Gabrilovich DI, Corak J, Ciernik IF, Kavanaugh D and Carbone DP: Decreased antigen presentation by dendritic cells in patients with breast cancer. Clin Cancer Res. 3:483–490. 1997.PubMed/NCBI

12 

Nestle FO, Burg G, Fah J, Wrone-Smith T and Nickoloff BJ: Human sunlight-induced basal-cell-carcinoma-associated dendritic cells are deficient in T cell co-stimulatory molecules and are impaired as antigen-presenting cells. Am J Pathol. 150:641–651. 1997.PubMed/NCBI

13 

Gabrilovich DI, Nadaf S, Corak J, Berzofsky JA and Carbone DP: Dendritic cells in antitumor immune responses. II Dendritic cells grown from bone marrow precursors, but not mature DC from tumor-bearing mice are effective antigen carriers in the therapy of established tumors. Cell Immunol. 170:111–119. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Gabrilovich DI, Chen HL, Girgis KR, et al: Production of vascular endothelial growth factor by human tumors inhibits the functional maturation of dendritic cells. Nat Med. 2:1096–1103. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Ratta M, Fagnoni F, Curti A, et al: Dendritic cells are functionally defective in multiple myeloma: the role of interleukin-6. Blood. 100:230–237. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Um SH, Mulhall C, Alisa A, et al: Alpha-fetoprotein impairs APC function and induces their apoptosis. J Immunol. 173:1772–1778. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Péguet-Navarro J, Sportouch M, Popa I, et al: Gangliosides from human melanoma tumors impair dendritic cell differentiation from monocytes and induce their apoptosis. J Immunol. 170:3488–3494. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Sombroek CC, Stam AG, Masterson AJ, et al: Prostanoids play a major role in the primary tumor-induced inhibition of dendritic cell differentiation. J Immunol. 168:4333–4343. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Bockorny B and Dasanu CA: Intrinsic immune alterations in renal cell carcinoma and emerging immunotherapeutic approaches. Expert Opin Biol Ther. 13:911–925. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Harimoto H, Shimizu M, Nakagawa Y, Nakatsuka K, Wakabayashi A, Sakamoto C and Takahashi H: Inactivation of tumor-specific CD8+ CTLs by tumor-infiltrating tolerogenic dendritic cells. Immunol Cell Biol. 91:545–555. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Toi M, Taniguchi T, Yamamoto Y, Kurisaki T, Suzuki H and Tominaga T: Clinical significance of the determination of angiogenic actors. Eur J Cancer. 32A:2513–2519. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Ellis LM and Fidler IJ: Angiogenesis and metastasis. Eur J Cancer. 32A:2451–2460. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Ferrara N and Davis-Smyth T: The biology of vascular endothelial growth factor. Endocr Rev. 18:4–25. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Salven P, Mänpää H, Orpana A, Alitalo K and Joensuu H: Serum vascular endothelial growth factor is often elevated in disseminated cancer. Clin Cancer Res. 3:647–651. 1997.PubMed/NCBI

25 

Ni YH, Wang ZY, Huang XF, et al: Effect of siRNA-mediated downregulation of VEGF in Tca8113 cells on the activity of monocyte-derived dendritic cells. Oncol Lett. 3:885–892. 2012.PubMed/NCBI

26 

Hajrasouliha AR, Funaki T, Sadrai Z, Hattori T, Chauhan SK and Dana R: Vascular endothelial growth factor-C promotes alloimmunity by amplifying antigen-presenting cell maturation and lymphangiogenesis. Invest Ophthalmol Vis Sci. 53:1244–1250. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Marti LC, Pavon L, Severino P, Sibov T, Guilhen D and Moreira-Filho CA: Vascular endothelial growth factor-A enhances indoleamine 2,3-dioxygenase expression by dendritic cells and subsequently impacts lymphocyte proliferation. Mem Inst Oswaldo Cruz. 109:70–79. 2014. View Article : Google Scholar :

28 

Liu CZ, Zhang L, Chang XH, et al: Overexpression and immunosuppressive functions of transforming growth factor 1, vascular endothelial growth factor and interleukin-10 in epithelial ovarian cancer. Chin J Cancer Res. 24:130–137. 2012. View Article : Google Scholar

29 

Takei Y, Kadomatsu K, Yuzawa Y, Matsuo S and Muramatsu T: A small interfering RNA targeting vascular endothelial growth factor as cancer therapeutics. Cancer Res. 64:3365–3370. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Walter S, Weinschenk T, Stenzl A, et al: Multipeptide immune response to cancer vaccine IMA901 after single-dose cyclophosphamide associates with longer patient survival. Nat Med. 18:1254–1261. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Cubillos-Ruiz JR, Baird JR, Tesone AJ, et al: Reprogramming tumor-associated dendritic cells in vivo using miRNA mimetics triggers protective immunity against ovarian cancer. Cancer Res. 72:1683–1693. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Gabrilovich DI, Ciernik IF and Carbone DP: Dendritic cells in antitumor immune responses. I Defective antigen presentation in tumor-bearing hosts. Cell Immunol. 170:101–110. 1996. View Article : Google Scholar : PubMed/NCBI

33 

Gabrilovich DI, Corak J, Ciernik IF, Kavanaugh D and Carbone DP: Decreased antigen presentation by dendritic cells in patients with breast cancer. Clin Cancer Res. 3:483–490. 1997.PubMed/NCBI

34 

Gabrilovich DI, Chen HL, Girgis KR, et al: Production of vascular endothelial growth factor by human tumors inhibits the functional maturation of dendritic cells. Nat Med. 2:1096–1103. 1996. View Article : Google Scholar : PubMed/NCBI

35 

Mosca PJ, Hobeika AC, Colling K, et al: Multiple signals are required for maturation of human dendritic cells mobilized in vivo with Flt3 ligand. J Leukoc Biol. 72:546–553. 2002.PubMed/NCBI

36 

Scarlett UK, Rutkowski MR, Rauwerdink AM, et al: Ovarian cancer progression is controlled by phenotypic changes in dendritic cells. J Exp Med. 209:495–506. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Gabrilovich DI, Chen HL, Girgis KR, et al: Production of vascular endothelial growth factor by human tumors inhibits the functional maturation of dendritic cells. Nat Med. 2:1096–1103. 1996. View Article : Google Scholar : PubMed/NCBI

38 

Woltman AM and van Kooten C: Functional modulation of dendritic cells to suppress adaptive immune responses. J Leukoc Biol. 73:428–441. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Dikov MM, Ohm JE, Ray N, et al: Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. J Immunol. 174:215–222. 2005. View Article : Google Scholar

40 

Leung DW, Cachianes G, Kuang WJ, Goeddel DV and Ferrara N: Vascular endothelial growth factor is a secreted angiogenic mitogen. Science. 246:1306–1309. 1989. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Zhang L, Zhang S and Li Y: Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells. Exp Ther Med 9: 120-124, 2015.
APA
Wang, H., Zhang, L., Zhang, S., & Li, Y. (2015). Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells. Experimental and Therapeutic Medicine, 9, 120-124. https://doi.org/10.3892/etm.2014.2059
MLA
Wang, H., Zhang, L., Zhang, S., Li, Y."Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells". Experimental and Therapeutic Medicine 9.1 (2015): 120-124.
Chicago
Wang, H., Zhang, L., Zhang, S., Li, Y."Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells". Experimental and Therapeutic Medicine 9, no. 1 (2015): 120-124. https://doi.org/10.3892/etm.2014.2059
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Zhang L, Zhang S and Li Y: Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells. Exp Ther Med 9: 120-124, 2015.
APA
Wang, H., Zhang, L., Zhang, S., & Li, Y. (2015). Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells. Experimental and Therapeutic Medicine, 9, 120-124. https://doi.org/10.3892/etm.2014.2059
MLA
Wang, H., Zhang, L., Zhang, S., Li, Y."Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells". Experimental and Therapeutic Medicine 9.1 (2015): 120-124.
Chicago
Wang, H., Zhang, L., Zhang, S., Li, Y."Inhibition of vascular endothelial growth factor by small interfering RNA upregulates differentiation, maturation and function of dendritic cells". Experimental and Therapeutic Medicine 9, no. 1 (2015): 120-124. https://doi.org/10.3892/etm.2014.2059
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