Open Access

In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

  • Authors:
    • Miao Dai
    • Peirong Xu
    • Min Hou
    • Yincheng Teng
    • QingKai Wu
  • View Affiliations

  • Published online on: November 27, 2014     https://doi.org/10.3892/etm.2014.2092
  • Pages: 372-376
  • Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to track in vivo the distribution and survival of adipose‑derived mesenchymal stem cells (ASCs) transplanted into female BALB/c nude mice following simulated childbirth injury, using green fluorescent protein and luciferase dual labeling, bioluminescent imaging (BLI) and histological evaluation. The results demonstrated that the dually labeled ASCs could be detected for up to eight weeks in vivo. The number of implanted cells decreased during the first three weeks, and then stabilized until the end of the experiment. According to the linear regression plot, ~27,621 implanted cells survived until eight weeks after implantation. Transplanted ASCs predominantly existed at the inoculation site of the vagina, with little or no spread to other organs. Histological analysis confirmed the survival of the engrafted ASCs. The study provided basic evidence that BLI techniques can be used to monitor ASCs in vivo in real time and in the long term. Through local administration, ASCs could survive in the long term to facilitate repair following pelvic‑floor injury.
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February-2015
Volume 9 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Dai M, Xu P, Hou M, Teng Y and Wu Q: In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury. Exp Ther Med 9: 372-376, 2015
APA
Dai, M., Xu, P., Hou, M., Teng, Y., & Wu, Q. (2015). In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury. Experimental and Therapeutic Medicine, 9, 372-376. https://doi.org/10.3892/etm.2014.2092
MLA
Dai, M., Xu, P., Hou, M., Teng, Y., Wu, Q."In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury". Experimental and Therapeutic Medicine 9.2 (2015): 372-376.
Chicago
Dai, M., Xu, P., Hou, M., Teng, Y., Wu, Q."In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury". Experimental and Therapeutic Medicine 9, no. 2 (2015): 372-376. https://doi.org/10.3892/etm.2014.2092