In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

Dai,Miao; Xu,Peirong; Hou,Min; Teng,Yincheng; Wu,QingKai

doi:10.3892/etm.2014.2092

February-2015 Volume 9 Issue 2

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February-2015 Volume 9 Issue 2

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Article Open Access

In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

Authors:
- Miao Dai
- Peirong Xu
- Min Hou
- Yincheng Teng
- QingKai Wu
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China

Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 372-376
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Published online on: November 27, 2014

https://doi.org/10.3892/etm.2014.2092
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Abstract

The aim of the present study was to track in vivo the distribution and survival of adipose‑derived mesenchymal stem cells (ASCs) transplanted into female BALB/c nude mice following simulated childbirth injury, using green fluorescent protein and luciferase dual labeling, bioluminescent imaging (BLI) and histological evaluation. The results demonstrated that the dually labeled ASCs could be detected for up to eight weeks in vivo. The number of implanted cells decreased during the first three weeks, and then stabilized until the end of the experiment. According to the linear regression plot, ~27,621 implanted cells survived until eight weeks after implantation. Transplanted ASCs predominantly existed at the inoculation site of the vagina, with little or no spread to other organs. Histological analysis confirmed the survival of the engrafted ASCs. The study provided basic evidence that BLI techniques can be used to monitor ASCs in vivo in real time and in the long term. Through local administration, ASCs could survive in the long term to facilitate repair following pelvic‑floor injury.

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1	Dannecker C and Anthuber C: The effects of childbirth on the pelvic-floor. J Perinat Med. 28:175–184. 2000. View Article : Google Scholar : PubMed/NCBI
2	Dietz HP and Wilson PD: Childbirth and pelvic floor trauma. Best Pract Res Clin Obstet Gynaecol. 19:913–924. 2005. View Article : Google Scholar : PubMed/NCBI
3	Chaliha C: Postpartum pelvic floor trauma. Curr Opin Obstet Gynecol. 21:474–479. 2009. View Article : Google Scholar : PubMed/NCBI
4	Wilson D, Dornan J, Milsom I, Freeman R, et al: UR-CHOICE: can we provide mothers-to-be with information about the risk of future pelvic floor dysfunction? Int Urogynecol J. 11:1449–1452. 2014. View Article : Google Scholar
5	Hagen S and Stark S: Conservative prevention and management of pelvic organ prolapse in women. Cochrane Database Syst Rev. Dec 7–2011.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
6	Carr LK, Robert M, Kultgen PL, et al: Autologous muscle derived cell therapy for stress urinary incontinence: a prospective, dose ranging study. J Urol. 189:595–601. 2013. View Article : Google Scholar
7	Lin CS and Lue TF: Stem cell therapy for stress urinary incontinence: a critical review. Stem Cells Dev. 21:834–843. 2012. View Article : Google Scholar :
8	Cruz M, Dissaranan C, Cotleur A, et al: Pelvic organ distribution of mesenchymal stem cells injected intravenously after simulated childbirth injury in female rats. Obstet Gynecol Int. 2012:6129462012. View Article : Google Scholar
9	Sen A, Lea-Currie YR, Sujkowska D, et al: Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous. J Cell Biochem. 81:312–319. 2001. View Article : Google Scholar : PubMed/NCBI
10	Park BS, Jang KA, Sung JH, et al: Adipose-derived stem cells and their secretory factors as a promising therapy for skin aging. Dermatol Surg. 34:1323–1326. 2008.PubMed/NCBI
11	Tobita M, Orbay H and Mizuno H: Adipose-derived stem cells: current findings and future perspectives. Discov Med. 11:160–170. 2011.PubMed/NCBI
12	Edalatmanesh MA, Bahrami AR, Hosseini E, Hosseini M and Khatamsaz S: Neuroprotective effects of mesenchymal stem cell transplantation in animal model of cerebellar degeneration. Neurol Res. 33:913–920. 2011. View Article : Google Scholar : PubMed/NCBI
13	Lin G, Wang G, Banie L, et al: Treatment of stress urinary incontinence with adipose tissue-derived stem cells. Cytotherapy. 12:88–95. 2010. View Article : Google Scholar :
14	Kinebuchi Y, Aizawa N, Imamura T, Ishizuka O, Igawa Y and Nishizawa O: Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. Int J Urol. 17:359–368. 2010. View Article : Google Scholar : PubMed/NCBI
15	Vilalta M, Dégano IR, Bagó J, et al: Biodistribution, long-term survival, and safety of human adipose tissue-derived mesenchymal stem cells transplanted in nude mice by high sensitivity non-invasive bioluminescence imaging. Stem Cells Dev. 17:993–1003. 2008. View Article : Google Scholar : PubMed/NCBI
16	Mitterberger M, Pinggera GM, Marksteiner R, et al: Adult stem cell therapy of female stress urinary incontinence. Eur Urol. 53:169–175. 2008. View Article : Google Scholar
17	Woo LL, Hijaz A, Kuang M, Penn MS, Damaser MS and Rackley RR: Over expression of stem cell homing cytokines in urogenital organs following vaginal distention. J Urol. 177:1568–1572. 2007. View Article : Google Scholar : PubMed/NCBI
18	Lenis AT, Kuang M, Woo LL, et al: Impact of parturition on chemokine homing factor expression in the vaginal distention model of stress urinary incontinence. J Urol. 189:1588–1594. 2013. View Article : Google Scholar
19	Bernardo ME, Pagliara D and Locatelli F: Mesenchymal stromal cell therapy: a revolution in Regenerative Medicine? Bone Marrow Transplant. 47:164–171. 2012. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

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Abstract

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