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Article

Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells

  • Authors:
    • Murat Pekmez
    • Evren Önay-Uçar
    • Nazlı Arda
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul 34134, Turkey
  • Pages: 585-590
    |
    Published online on: December 3, 2014
       https://doi.org/10.3892/etm.2014.2101
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Abstract

Indomethacin is a member of the non-steroidal anti‑inflammatory drug (NSAID) class, which has great potential for use in the treatment of glioma. However, it induces the generation of reactive oxygen species (ROS) and causes molecular damage while inducing its effects. Vitamin E is widely used in the complementary therapy of cancers. The main goal of the present study was to investigate the effects of α-tocopheryl succinate (α-TOS) against the oxidative damage induced by indomethacin in C6 glioma cells. Cells were treated with 10 µM α-TOS alone or in combination with 200 µM indomethacin for two days. The intracellular ROS level, molecular damage as revealed by lipid peroxidation and protein carbonyl formation, and the COX activity in C6 glioma cells were measured. Treatment of the cells with α-TOS and indomethacin, alone or in combination, caused the levels of ROS generation and protein damage to increase, but protected against lipid peroxidation and reduced COX activity.
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Copy and paste a formatted citation
Spandidos Publications style
Pekmez M, Önay-Uçar E and Arda N: Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells. Exp Ther Med 9: 585-590, 2015.
APA
Pekmez, M., Önay-Uçar, E., & Arda, N. (2015). Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells. Experimental and Therapeutic Medicine, 9, 585-590. https://doi.org/10.3892/etm.2014.2101
MLA
Pekmez, M., Önay-Uçar, E., Arda, N."Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells". Experimental and Therapeutic Medicine 9.2 (2015): 585-590.
Chicago
Pekmez, M., Önay-Uçar, E., Arda, N."Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells". Experimental and Therapeutic Medicine 9, no. 2 (2015): 585-590. https://doi.org/10.3892/etm.2014.2101
Copy and paste a formatted citation
x
Spandidos Publications style
Pekmez M, Önay-Uçar E and Arda N: Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells. Exp Ther Med 9: 585-590, 2015.
APA
Pekmez, M., Önay-Uçar, E., & Arda, N. (2015). Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells. Experimental and Therapeutic Medicine, 9, 585-590. https://doi.org/10.3892/etm.2014.2101
MLA
Pekmez, M., Önay-Uçar, E., Arda, N."Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells". Experimental and Therapeutic Medicine 9.2 (2015): 585-590.
Chicago
Pekmez, M., Önay-Uçar, E., Arda, N."Effect of α-tocopheryl succinate on the molecular damage induced by indomethacin in C6 glioma cells". Experimental and Therapeutic Medicine 9, no. 2 (2015): 585-590. https://doi.org/10.3892/etm.2014.2101
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