Open Access

Curcumin protects against lipopolysaccharide-induced vasoconstriction dysfunction via inhibition of thrombospondin-1 and transforming growth factor-β1

  • Authors:
    • Wei Lu
    • Jian‑Ping Jiang
    • Jue Hu
    • Jue Wang
    • Ming‑Zhi Zheng
  • View Affiliations

  • Published online on: December 3, 2014     https://doi.org/10.3892/etm.2014.2105
  • Pages: 377-383
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sepsis is a complex syndrome characterized by the development of progressive dysfunction in multiple organs. The aim of the present study was to investigate the protective effect of curcumin against lipopolysaccharide (LPS)‑induced vasoconstrictive dysfunction, and to investigate the possible underlying mechanism. Male Sprague‑Dawley rats were randomly divided into the following groups: Control, sepsis and curcumin. A sepsis model was established by an intraperitoneal (i.p.) injection of 5 mg/kg LPS. Thoracic aortic rings obtained from the rats were mounted in an organ bath and the vasoconstriction of the rings was recorded. In addition, the serum E‑selectin levels were determined by an enzyme‑linked immunosorbent assay. The expression levels of thrombospondin (TSP)‑1 and transforming growth factor (TGF)‑β1 in the aortic tissue were detected by immunohistochemistry. Vasoconstriction of the aortic rings was found to significantly decrease in the sepsis rats when compared with the control group. However, curcumin (10 or 20 mg/kg, i.p.) prevented the vasoconstrictive dysfunction induced by LPS. The serum level of E‑selectin and the expression levels of TSP‑1 and TGF‑β1 significantly increased in the sepsis rats when compared with the control group rats; however, the levels decreased significantly following treatment with curcumin (10 or 20 mg/kg). Furthermore, hematoxylin and eosin staining revealed that curcumin alleviated the LPS‑induced damage in the aortic tunica intima and tunica media. Therefore, the results indicated that curcumin alleviates LPS‑induced vasoconstrictive dysfunction in the thoracic aorta of rats. In addition, the inhibition of TSP‑1 and TGF‑β1 expression may be involved in the mechanism underlying this protective effect.
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February-2015
Volume 9 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lu W, Jiang JP, Hu J, Wang J and Zheng MZ: Curcumin protects against lipopolysaccharide-induced vasoconstriction dysfunction via inhibition of thrombospondin-1 and transforming growth factor-β1. Exp Ther Med 9: 377-383, 2015
APA
Lu, W., Jiang, J., Hu, J., Wang, J., & Zheng, M. (2015). Curcumin protects against lipopolysaccharide-induced vasoconstriction dysfunction via inhibition of thrombospondin-1 and transforming growth factor-β1. Experimental and Therapeutic Medicine, 9, 377-383. https://doi.org/10.3892/etm.2014.2105
MLA
Lu, W., Jiang, J., Hu, J., Wang, J., Zheng, M."Curcumin protects against lipopolysaccharide-induced vasoconstriction dysfunction via inhibition of thrombospondin-1 and transforming growth factor-β1". Experimental and Therapeutic Medicine 9.2 (2015): 377-383.
Chicago
Lu, W., Jiang, J., Hu, J., Wang, J., Zheng, M."Curcumin protects against lipopolysaccharide-induced vasoconstriction dysfunction via inhibition of thrombospondin-1 and transforming growth factor-β1". Experimental and Therapeutic Medicine 9, no. 2 (2015): 377-383. https://doi.org/10.3892/etm.2014.2105