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Article

Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes

  • Authors:
    • Yong‑Qin Li
    • Rui Liu
    • Jia‑Hong Xue
    • Yan Zhang
    • Deng‑Feng Gao
    • Xiao‑San Wu
    • Cong‑Xia Wang
    • Yu‑Bai Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Physiology and Pathophysiology, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061 P.R. China
  • Pages: 527-532
    |
    Published online on: December 5, 2014
       https://doi.org/10.3892/etm.2014.2109
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Abstract

The adhesion of monocytes to endothelial cells is one of the early stages in the development of atherosclerosis. The expression of type IV collagenases, which include matrix metalloproteinase (MMP)‑2 and MMP‑9, in monocytes is hypothesized to play an important role in monocyte infiltration and transformation into foam cells. The aim of the present study was to examine the effects of monocyte‑endothelium interactions on the expression levels of type IV collagenases and their specific inhibitors in monocytes, and to investigate the roles of tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β in this process. Monocytes were single‑cultured or co‑cultured with endothelial cells. The expression of the type IV collagenases, MMP‑2 and MMP‑9, and their specific inhibitors, tissue inhibitor of metalloproteinase (TIMP)‑1 and TIMP‑2, in monocytes was determined by immunohistochemistry followed by image analysis. The expression levels of MMP‑2 and MMP‑9 were found to be low in the single‑culture monocytes, but increased significantly when the monocytes and endothelial cells were co‑cultured. However, treatment with monoclonal TNF‑α or IL‑1β antibodies partially inhibited the upregulated expression of MMP‑2 and MMP‑9 in the co‑cultured monocytes. Expression of TIMP‑1 and TIMP‑2 was observed in the single monocyte culture, and a small increase in the expression levels was observed when the monocytes were co‑cultured with endothelial cells. Therefore, monocyte‑endothlium interactions were shown to increase the expression of type IV collagenases in monocytes, resulting in the loss of balance between MMP‑2 and ‑9 with TIMP‑1 and ‑2. In addition, TNF‑α and IL‑1β were demonstrated to play important roles in this process.
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Copy and paste a formatted citation
Spandidos Publications style
Li YQ, Liu R, Xue JH, Zhang Y, Gao DF, Wu XS, Wang CX and Yang YB: Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes. Exp Ther Med 9: 527-532, 2015.
APA
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X. ... Yang, Y. (2015). Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes. Experimental and Therapeutic Medicine, 9, 527-532. https://doi.org/10.3892/etm.2014.2109
MLA
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X., Wang, C., Yang, Y."Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes". Experimental and Therapeutic Medicine 9.2 (2015): 527-532.
Chicago
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X., Wang, C., Yang, Y."Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes". Experimental and Therapeutic Medicine 9, no. 2 (2015): 527-532. https://doi.org/10.3892/etm.2014.2109
Copy and paste a formatted citation
x
Spandidos Publications style
Li YQ, Liu R, Xue JH, Zhang Y, Gao DF, Wu XS, Wang CX and Yang YB: Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes. Exp Ther Med 9: 527-532, 2015.
APA
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X. ... Yang, Y. (2015). Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes. Experimental and Therapeutic Medicine, 9, 527-532. https://doi.org/10.3892/etm.2014.2109
MLA
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X., Wang, C., Yang, Y."Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes". Experimental and Therapeutic Medicine 9.2 (2015): 527-532.
Chicago
Li, Y., Liu, R., Xue, J., Zhang, Y., Gao, D., Wu, X., Wang, C., Yang, Y."Effects of monocyte-endothelium interactions on the expression of type IV collagenases in monocytes". Experimental and Therapeutic Medicine 9, no. 2 (2015): 527-532. https://doi.org/10.3892/etm.2014.2109
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