A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

Qiao,Jianliang; Han,Chunlei; Zhang,Junjing; Wang,Zhiyong; Meng,Xingkai

doi:10.3892/etm.2014.2110

February-2015 Volume 9 Issue 2

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February-2015 Volume 9 Issue 2

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Article Open Access

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

Authors:
- Jianliang Qiao
- Chunlei Han
- Junjing Zhang
- Zhiyong Wang
- Xingkai Meng
View Affiliations / Copyright

Affiliations: Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, Turku PET Centre, Turku University Hospital and University of Turku, Turku 20521, Finland, Department of General Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China

Copyright: © Qiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 367-371
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Published online on: December 5, 2014

https://doi.org/10.3892/etm.2014.2110
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Abstract

Auxiliary partial heterotopic liver transplantation (APHLT) with portal vein arterialization is a valuable procedure to be considered in the treatment of patients with acute liver failure and metabolic liver diseases. The aim of this study was to develop a new rat model of APHLT with liver dual arterial blood supply (LDABS). A total of 20 rats were used. The donor liver was resected, and the celiac trunk was reserved. Left and medial hepatic lobes accounting for 70% of the liver mass were removed en bloc and the suprahepatic caval vein was ligated simultaneously. Thus, 30% of the donor liver was obtained as the graft. Sleeve anastomosis of the graft portal vein and splenic artery were performed after narrowing the portal vein lumen through suturing. The right kidney of the recipient was removed, and sleeve anastomosis was performed between the celiac trunk of the graft and the right renal artery of the recipient. In addition, end‑to‑end anastomosis was performed between the infrahepatic caval vein of the graft and the right renal vein of the recipient. Following the reperfusion of the graft, the blood flow of the arterialized portal vein was controlled within the physiological range through suturing and narrowing under monitoring with an ultrasonic flowmeter. The bile duct of the graft was implanted into the duodenum of the recipient through an internal stent catheter. A 70% section of the native liver (left and medial hepatic lobes) was resected using bloodless hepatectomy. The mean operative duration was 154.5±16.4 min, and the warm and cold ischemia times of the graft were 8.1±1.1 min and 64.5±6.6 min, respectively. The blood flow of the arterialized portal vein to the graft was 1.8±0.3 ml/min/g liver weight. The success rate of model establishment (waking with post‑surgical survival of >24 h) was 70% (7/10). Following successful model establishment, all rats survived 7 days post‑surgery (100%; 7/7). The graft was found to be soft in texture and bright red in color following exploratory laparotomy. In conclusion, a new rat model of APHLT with LDABS without stent for vascular reconstruction was developed. This is a feasible and reliable rat model for liver transplantation study.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

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