Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-2015 Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2015 Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.

  • Authors:
    • Peng Deng
    • Xiaoqing Tan
    • Ying Wu
    • Qunhua Bai
    • Yan Jia
    • Hong Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Occupational and Environmental Health, College of Public Health and Administration, Chongqing Medical University, Chongqing 400016, P.R. China, Physical Examination Center, Third People's Hospital of Chongqing, Chongqing 401121, P.R. China
  • Pages: 795-800
    |
    Published online on: December 18, 2014
       https://doi.org/10.3892/etm.2014.2148
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The ChrT gene encodes a chromate reductase enzyme which catalyzes the reduction of Cr(VI). The chromate reductase is also known as flavin mononucleotide (FMN) reductase (FMN_red). The aim of the present study was to clone the full‑length ChrT DNA from Serratia sp. CQMUS2 and analyze the deduced amino acid sequence and three‑dimensional structure. The putative ChrT gene fragment of Serratia sp. CQMUS2 was isolated by polymerase chain reaction (PCR), according to the known FMN_red gene sequence from Serratia sp. AS13. The flanking sequences of the ChrT gene were obtained by high efficiency TAIL‑PCR, while the full‑length gene of ChrT was cloned in Escherichia coli for subsequent sequencing. The nucleotide sequence of ChrT was submitted onto GenBank under the accession number, KF211434. Sequence analysis of the gene and amino acids was conducted using the Basic Local Alignment Search Tool, and open reading frame (ORF) analysis was performed using ORF Finder software. The ChrT gene was found to be an ORF of 567 bp that encodes a 188‑amino acid enzyme with a calculated molecular weight of 20.4 kDa. In addition, the ChrT protein was hypothesized to be an NADPH‑dependent FMN_red and a member of the flavodoxin‑2 superfamily. The amino acid sequence of ChrT showed high sequence similarity to the FMN reductase genes of Klebsiella pneumonia and Raoultella ornithinolytica, which belong to the flavodoxin‑2 superfamily. Furthermore, ChrT was shown to have a 85.6% similarity to the three‑dimensional structure of Escherichia coli ChrR, sharing four common enzyme active sites for chromate reduction. Therefore, ChrT gene cloning and protein structure determination demonstrated the ability of the gene for chromate reduction. The results of the present study provide a basis for further studies on ChrT gene expression and protein function.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ren BQ, Wang YL, Zhao LY and Jin Y: Actuality and trend of chromium containing wastewater treatment technology. Heilongjiang Kexue. 4:67–69. 2013.(In Chinese).

2 

Robins KJ, Hooks DO, Rehm BH and Ackerley DF: Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium. PLoS One. 8:e592002013. View Article : Google Scholar : PubMed/NCBI

3 

Tahri Joutey N, Bahafid W, Sayel H, Ananou S and El Ghachtouli N: Hexavalent chromium removal by a novel Serratia proteamaculans isolated from the bank of Sebou River (Morocco). Environ Sci Pollut Res Int. 21:3060–3072. 2014. View Article : Google Scholar

4 

Christl I, Imseng M, Tatti E, Frommer J, Viti C, Giovannetti L and Kretzschmar R: Aerobic reduction of chromium (VI) by Pseudomonas corrugata 28: Influence of metabolism and fate of reduced chromium. Geomicrobiol J. 29:173–185. 2012. View Article : Google Scholar

5 

Zhitkovich A: Chromium in drinking water: sources, metabolism, and cancer risks. Chem Res Toxicol. 24:1617–1629. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Ramírez-Díaz MI, Díaz-Pérez C, Vargas E, Riveros-Rosas H, Campos-García J and Cervantes C: Mechanisms of bacterial resistance to chromium compounds. Biometals. 21:321–332. 2008. View Article : Google Scholar

7 

Sandana Mala JG, Sujatha D and Rose C: Inducible chromate reductase exhibiting extracellular activity in Bacillus methylotrophicus for chromium bioremediation. Microbiol Res. pii: S0944-5013(14)00067-6. 2014.PubMed/NCBI

8 

Arévalo-Rangel DL, Cárdenas-González JF, Martínez-Juárez VM and Acosta-Rodríguez I: Hexavalent chromate reductase activity in cell free extracts of Penicillium sp. Bioinorg Chem Appl. 2013:9094122013. View Article : Google Scholar : PubMed/NCBI

9 

Belchik SM, Kennedy DW, Dohnalkova AC, et al: Extracellular reduction of hexavalent chromium by cytochromes MtrC and OmcA of Shewanella oneidensis MR-1. Appl Environ Microbiol. 77:4035–4041. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Park CH, Keyhan M, Wielinga B, Fendorf S and Matin A: Purification to homogeneity and characterization of a novel Pseudomonas putida chromate reductase. Appl Environ Microbiol. 66:1788–1795. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Ackerley DF, Gonzalez CF, Park CH, Blake R 2nd, Keyhan M and Matin A: Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol. 70:873–882. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Agarwal R, Bonnano JB, Burley SK and Swaminathan S: Structure determination of an FMN-reductase from Pseudomonas aeruginosa PAO1 using sulfur anomalous signal. Acta Crystallogr D Biol Crystallogr. 62:383–391. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Tan XQ, Deng P, Wu Y, et al: In vitro synthesis and activity identification of chromate reductase T. Hubei Yixueyuan Xuebao. 7:973–977. 2014.(In Chinese).

14 

Sun YB, Mo DJ and Dai TL: Modifying methods of preparation of PCR template and recovery of DNA. Acta Academiae Medicinae Hubei. 18:24–25. 1997.(In Chinese).

15 

Liu YG and Chen YL: High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. Biotechniques. 43:649–650. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Clustal X version 2.0. http://www.ebi.ac.uk/tools/clustalw2uri. Accessed 27/07/2013

17 

Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.2. http://www.megasoftware.net/uri. Accessed 30/09/2013

18 

Wang J, Zhang H, Wu M and Tang C: Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii. Biotechnol Lett. 33:1029–1038. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Araya-Garay JM, Feijoo-Siota L, Veiga-Crespo P and Villa TG: cDNA cloning of a novel gene codifying for the enzyme lycopene β-cyclase from Ficus carica and its expression in Escherichia coli. Appl Microbiol Biotechnol. 92:769–777. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Pang X, Zhou DS and Yang RF: Summary of bacteria mRNA extraction. Shengwu Jishu Tongbao. 1:30–34. 2003.(In Chinese).

21 

Tan HQ and Singh J: High-efficiency thermal asymmetric interlaced (HE-TAIL) PCR for amplification of Ds transposon insertion sites in Barley. J Plant Mol Biol Biotechnol. 2:9–14. 2011.

22 

Jin H, Zhang Y, Buchko GW, et al: Structure determination and functional analysis of a chromate reductase from Gluconacetobacter hansenii. PLoS One. 7:e424322012. View Article : Google Scholar : PubMed/NCBI

23 

Eswaramoorthy S, Poulain S, Hienerwadel R, et al: Crystal structure of ChrR - a quinone reductase with the capacity to reduce chromate. PLoS One. 7:e360172012. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Deng P, Tan X, Wu Y, Bai Q, Jia Y and Xiao H: Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.. Exp Ther Med 9: 795-800, 2015.
APA
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., & Xiao, H. (2015). Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.. Experimental and Therapeutic Medicine, 9, 795-800. https://doi.org/10.3892/etm.2014.2148
MLA
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., Xiao, H."Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.". Experimental and Therapeutic Medicine 9.3 (2015): 795-800.
Chicago
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., Xiao, H."Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.". Experimental and Therapeutic Medicine 9, no. 3 (2015): 795-800. https://doi.org/10.3892/etm.2014.2148
Copy and paste a formatted citation
x
Spandidos Publications style
Deng P, Tan X, Wu Y, Bai Q, Jia Y and Xiao H: Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.. Exp Ther Med 9: 795-800, 2015.
APA
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., & Xiao, H. (2015). Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.. Experimental and Therapeutic Medicine, 9, 795-800. https://doi.org/10.3892/etm.2014.2148
MLA
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., Xiao, H."Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.". Experimental and Therapeutic Medicine 9.3 (2015): 795-800.
Chicago
Deng, P., Tan, X., Wu, Y., Bai, Q., Jia, Y., Xiao, H."Cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from Serratia sp.". Experimental and Therapeutic Medicine 9, no. 3 (2015): 795-800. https://doi.org/10.3892/etm.2014.2148
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team