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Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics

  • Authors:
    • Zhiying Tian
    • Zhibang Yang
    • Jiye Gao
    • Lili Zhu
    • Renju Jiang
    • Ying Jiang

  • View Affiliations

  • Published online on: January 7, 2015     https://doi.org/10.3892/etm.2015.2169
  • Pages: 685-692

  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of this study was to investigate the effect of Helicobacter pylori (H. pylori) infection on the lower esophageal microbiota and the eradication of H. pylori through the use of antibiotics. Forty‑five BALB/C mice were randomly divided into negative control, infection and treatment groups. The mice were sacrificed and DNA was extracted from the lower esophageal microbiota. Polymerase chain reaction‑denaturing gradient gel electrophoresis (PCR‑DGGE) was performed to determine the composition of the microbiota. Quantity One® 1‑D Analysis Software was used for the analysis of the DGGE profiles. The different bands from the groups were amplified with 16S rDNA V6 region primers. DNA sequencing and Basic Local Alignment Search Tool analysis were performed for the identification of the bands. H. pylori colonization led to severe ulcers in the stomachs of the mice, and these ulcers were alleviated by antibiotic treatment. The infection group had an increased number of bacterial species in the stomach compared with the control and treatment groups. DGGE fingerprinting of the lower esophagus showed that there were significant differences in the number of bands (P<0.05), diversity index and abundance among the groups (P<0.05); however, no significant differences in homogeneity were observed (P>0.05). Although the composition of flora species in the lower espohagus varied, the dominant species, and their relative contents, were similar in the control, infection and treatment groups. The present study provided a microecological basis for the understanding of the pathogenesis of lower esophageal diseases, following the eradication of H. pylori infection with antibiotics.
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March-2015
Volume 9 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tian Z, Yang Z, Gao J, Zhu L, Jiang R and Jiang Y: Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics. Exp Ther Med 9: 685-692, 2015
APA
Tian, Z., Yang, Z., Gao, J., Zhu, L., Jiang, R., & Jiang, Y. (2015). Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics. Experimental and Therapeutic Medicine, 9, 685-692. https://doi.org/10.3892/etm.2015.2169
MLA
Tian, Z., Yang, Z., Gao, J., Zhu, L., Jiang, R., Jiang, Y."Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics". Experimental and Therapeutic Medicine 9.3 (2015): 685-692.
Chicago
Tian, Z., Yang, Z., Gao, J., Zhu, L., Jiang, R., Jiang, Y."Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics". Experimental and Therapeutic Medicine 9, no. 3 (2015): 685-692. https://doi.org/10.3892/etm.2015.2169