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Article

Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease

  • Authors:
    • Cuige Liang
    • Wenhua Du
    • Qingyu Dong
    • Xiaomeng Liu
    • Wenxia Li
    • Yueli Wang
    • Guanqi Gao
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China
  • Pages: 925-930
    |
    Published online on: January 14, 2015
       https://doi.org/10.3892/etm.2015.2180
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Abstract

The aim of the present study was to determine whether the expression levels of interleukin (IL)‑2 and IL‑10 may be used as biological markers in Graves' disease (GD) patients. A total of 256 individuals, including 118 GD patients and 138 healthy individuals, were enrolled into the study. Blood samples were collected from each patient and healthy individual, which were then subjected to enzyme‑linked immunosorbent assay (ELISA). Total RNA and total proteins were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis, respectively. In addition, restriction fragment length polymorphism (RFLP) analysis was performed to detect the pre­sence of genetic polymorphisms. The ELISA results indicated that the IL‑2 and IL‑10 serum levels in the GD patients were increased by ~5.2 and ~7‑fold when compared with the levels in the healthy controls. The results of RT‑qPCR indicated that the mRNA expression levels of IL‑2 and IL‑10 were upregulated in the GD patients when compared with the healthy controls. Furthermore, the western blot analysis results revealed that the protein expression levels of IL‑2 and IL‑10 were significantly increased in the GD patients. RFLP analysis indicated that the increased number of GG single nucleotide polymorphisms (SNPs) in the GD group were detected in the ‑330 locus of the IL‑2 promoter and the ‑1082 locus of the IL‑10 promoter. In addition, the results indicated that the relatively high rates of homozygous GG SNPs (IL‑2 ‑330T/G and IL‑10 ‑1082A/G polymorphisms) on the alleles may be associated with the incidence of GD. The serum, mRNA and protein expression levels of IL‑2 and IL‑10 were significantly increased in GD patients when compared with the levels in the healthy controls. In conclusion, the expression levels and genetic polymorphisms of IL‑2 and IL‑10 may be potential biomarkers for the incidence of Graves' disease in the population studied.
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Copy and paste a formatted citation
Spandidos Publications style
Liang C, Du W, Dong Q, Liu X, Li W, Wang Y and Gao G: Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease. Exp Ther Med 9: 925-930, 2015.
APA
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., & Gao, G. (2015). Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease. Experimental and Therapeutic Medicine, 9, 925-930. https://doi.org/10.3892/etm.2015.2180
MLA
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., Gao, G."Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease". Experimental and Therapeutic Medicine 9.3 (2015): 925-930.
Chicago
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., Gao, G."Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease". Experimental and Therapeutic Medicine 9, no. 3 (2015): 925-930. https://doi.org/10.3892/etm.2015.2180
Copy and paste a formatted citation
x
Spandidos Publications style
Liang C, Du W, Dong Q, Liu X, Li W, Wang Y and Gao G: Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease. Exp Ther Med 9: 925-930, 2015.
APA
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., & Gao, G. (2015). Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease. Experimental and Therapeutic Medicine, 9, 925-930. https://doi.org/10.3892/etm.2015.2180
MLA
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., Gao, G."Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease". Experimental and Therapeutic Medicine 9.3 (2015): 925-930.
Chicago
Liang, C., Du, W., Dong, Q., Liu, X., Li, W., Wang, Y., Gao, G."Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease". Experimental and Therapeutic Medicine 9, no. 3 (2015): 925-930. https://doi.org/10.3892/etm.2015.2180
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