Comparative proteomic analysis of membranous nephropathy biopsy tissues using quantitative proteomics

Sui,Weiguo; Zhang,Ruohan; Chen,Jiejing; He,Huiyan; Cui,Zhenzhen; Ou,Minglin; Guo,Li; Cong,Shan; Xue,Wen; Dai,Yong

doi:10.3892/etm.2015.2197

March-2015 Volume 9 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

March-2015 Volume 9 Issue 3

Full Size Image

Article

Comparative proteomic analysis of membranous nephropathy biopsy tissues using quantitative proteomics

Authors:
- Weiguo Sui
- Ruohan Zhang
- Jiejing Chen
- Huiyan He
- Zhenzhen Cui
- Minglin Ou
- Li Guo
- Shan Cong
- Wen Xue
- Yong Dai
View Affiliations / Copyright

Affiliations: Nephrology Department, Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Disease Research, Guilin, Guangxi 541002, P.R. China
Pages: 805-810
|
Published online on: January 21, 2015

https://doi.org/10.3892/etm.2015.2197
Expand metrics +

Abstract

Membranous nephropathy (MN) is a common cause of nephrotic syndrome in adults and the second leading cause of end‑stage renal disease due to primary glomerulonephritis. The aim of the present study was to identify potential biomarkers of MN and further characterize these proteins by Gene Ontology (GO) analysis. Isobaric tags for relative and absolute quantification were used to compare the protein levels in tissues from MN patients and healthy individuals, and the combined samples were subsequently separated by specialized communications exchange. Mass spectrometry data acquisition was conducted using a 4800 Plus MALDI TOF/TOF tandem mass spectrometry device, and the results were subjected to statistical analysis. A total of 1,903 proteins were identified, with 423 proteins exhibiting a difference of >1.5‑fold compared with the control group. Of these, 202 proteins were upregulated, while 221 proteins were downregulated. In conclusion, GO enrichment analysis revealed that the differentially expressed proteins were primarily mapped to the following GO terms: ‘Immune response’, ‘immune effector process’, ‘activation of immune response’ and ‘positive regulation of immune system process’. The affected proteins may be associated with the pathogenesis of MN; thus, may represent candidate MN biomarkers.

View Figures

View References

1	Papasotiriou M, Kalliakmani P, Huang L, et al: Does treatment with corticosteroids and cyclosporine reduce transglutaminase type 2 expression in the renal tissue of patients with membranous nephropathy? Nephron Clin Pract. 121:c60–c67. 2012. View Article : Google Scholar : PubMed/NCBI
2	Beck LH Jr, Bonegio RG, Lambeau G, et al: M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy. N Engl J Med. 361:11–21. 2009. View Article : Google Scholar : PubMed/NCBI
3	Ngai HH, Sit WH, Jiang PP, et al: Serial changes in urinary proteome profile of membranous nephropathy: implications for pathophysiology and biomarker discovery. J Proteome Res. 5:3038–3047. 2006. View Article : Google Scholar : PubMed/NCBI
4	Thongboonkerd V: Biomarker discovery in glomerular diseases using urinary proteomics. Proteomics Clin Appl. 2:1413–1421. 2008. View Article : Google Scholar : PubMed/NCBI
5	Whittier WL and Korbet SM: Renal biopsy: update. Curr Opin Nephrol Hypertens. 13:661–665. 2004. View Article : Google Scholar : PubMed/NCBI
6	Veenstra TD: Global and targeted quantitative proteomics for biomarker discovery. J Chromatogr B Analyt Technol Biomed Life Sci. 847:3–11. 2007. View Article : Google Scholar
7	Duncan MW and Hunsucker SW: Proteomics as a tool for clinically relevant biomarker discovery and validation. Exp Biol Med (Maywood). 230:808–817. 2005.
8	Sun C, Song C, Ma Z, et al: Periostin identified as a potential biomarker of prostate cancer by iTRAQ-proteomics analysis of prostate biopsy. Proteome Sci. 9:222011. View Article : Google Scholar : PubMed/NCBI
9	Sui W, Tang D, Zou G, et al: Differential proteomic analysis of renal tissue in lupus nephritis using iTRAQ reagent technology. Rheumatol Int. 32:3537–3543. 2012. View Article : Google Scholar
10	DeSouza L, Diehl G, Rodrigues MJ, et al: Search for cancer markers from endometrial tissues using differentially labeled tags iTRAQ and cICAT with multidimensional liquid chromatography and tandem mass spectrometry. J Proteome Res. 4:377–386. 2005. View Article : Google Scholar : PubMed/NCBI
11	Ralhan R, Desouza LV, Matta A, et al: Discovery and verification of head-and-neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry. Mol Cell Proteomics. 7:1162–1173. 2008. View Article : Google Scholar : PubMed/NCBI
12	Chen JS, Chen KT, Fan CW, et al: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry. FEBS J. 277:3028–3038. 2010. View Article : Google Scholar : PubMed/NCBI
13	Irazabal MV, Eirin A, Lieske J, et al: Low- and high-molecular-weight urinary proteins as predictors of response to rituximab in patients with membranous nephropathy: a prospective study. Nephrol Dial Transplant. 28:137–146. 2013. View Article : Google Scholar :
14	Wang L, Dai Y, Qi S, et al: Comparative proteome analysis of peripheral blood mononuclear cells in systemic lupus erythematosus with iTRAQ quantitative proteomics. Rheumatol Int. 32:585–593. 2012. View Article : Google Scholar
15	Hirayama K, Ebihara I, Yamamoto S, et al: Predominance of type-2 immune response in idiopathic membranous nephropathy. Cytoplasmic cytokine analysis. Nephron. 91:255–261. 2002. View Article : Google Scholar : PubMed/NCBI
16	Ronco P and Debiec H: Antigen identification in membranous nephropathy moves toward targeted monitoring and new therapy. J Am Soc Nephrol. 21:564–569. 2010. View Article : Google Scholar : PubMed/NCBI
17	Werber HI, Emancipator SN, Tykocinski ML and Sedor JR: The interleukin 1 gene is expressed by rat glomerular mesangial cells and is augmented in immune complex glomerulonephritis. J Immunol. 138:3207–3212. 1987.PubMed/NCBI
18	Hofstra JM, Deegens JK, Willems HL and Wetzels JF: Beta-2-microglobulin is superior to N-acetyl-beta-glucosaminidase in predicting prognosis in idiopathic membranous nephropathy. Nephrol Dial Transplant. 23:2546–2551. 2008. View Article : Google Scholar : PubMed/NCBI
19	Branten AJ, du Buf-Vereijken PW, Klasen IS, et al: Urinary excretion of beta2-microglobulin and IgG predict prognosis in idiopathic membranous nephropathy: a validation study. J Am Soc Nephrol. 16:169–174. 2005. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparative proteomic analysis of membranous nephropathy biopsy tissues using quantitative proteomics

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles