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Article

Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression

  • Authors:
    • Xihai Li
    • Jiashou Chen
    • Wenna Liang
    • Huiting Li
    • Fayuan Liu
    • Xiaping Weng
    • Pingdong  Lin
    • Wenlie Chen
    • Chunsong Zheng
    • Huifeng Xu
    • Xianxiang Liu
    • Hongzhi Ye
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
  • Pages: 839-844
    |
    Published online on: January 23, 2015
       https://doi.org/10.3892/etm.2015.2214
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Abstract

Bushen Zhuangjin Decoction (BZD), a well‑known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose‑ and time‑dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin‑dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription‑polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD‑treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA.
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1 

Juhl C, Christensen R, Roos EM, Zhang W and Lund H: Impact of exercise type and dose on pain and disability in knee osteoarthritis: a systematic review and meta-regression analysis of randomized controlled trials. Arthritis Rheumatol. 66:622–636. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Zhang W, Nuki G, Moskowitz RW, et al: OARSI recommendations for the management of hip and knee osteoarthritis: part III: Changes in evidence following systematic cumulative update of research published through January 2009. Osteoarthritis Cartilage. 18:476–499. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Tonge DP, Pearson MJ and Jones SW: The hallmarks of osteoarthritis and the potential to develop personalised disease-modifying pharmacological therapeutics. Osteoarthritis Cartilage. 22:609–621. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Li X, Ye H, Yu F, et al: Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition. Int J Mol Med. 29:823–831. 2012.PubMed/NCBI

5 

Kurimchak A, Haines DS, Garriga J, et al: Activation of p107 by fibroblast growth factor, which is essential for chondrocyte cell cycle exit, is mediated by the protein phosphatase 2A/B55α holoenzyme. Mol Cell Biol. 33:3330–3342. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Lee HP, Chen YL, Shen HC, Lo WH and Hu YC: Baculovirus transduction of rat articular chondrocytes: roles of cell cycle. J Gene Med. 9:33–43. 2007. View Article : Google Scholar

7 

Panda DK, Miao D, Lefebvre V, Hendy GN and Goltzman D: The transcription factor SOX9 regulates cell cycle and differentiation genes in chondrocytic CFK2 cells. J Biol Chem. 276:41229–41236. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Lambert RG, Hutchings EJ, Grace MG, Jhangri GS, Conner-Spady B and Maksymowych WP: Steroid injection for osteoarthritis of the hip: a randomized, double-blind, placebo-controlled trial. Arthritis Rheum. 56:2278–2287. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Yang S, Dubé CE, Eaton CB, McAlindon TE and Lapane KL: Longitudinal use of complementary and alternative medicine among older adults with radiographic knee osteoarthritis. Clin Ther. 35:1690–1702. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li XH, Wu MX, Ye HZ, et al: Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong capsule-containing serum. Clin J Integr Med. 17:436–443. 2011. View Article : Google Scholar

11 

Li X, Lang W, Ye H, et al: Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy. Int J Mol Med. 31:1349–1356. 2013.PubMed/NCBI

12 

Zhou GS, Li XF and Guan GH: Effects of Bushen Zhuangjin Decoction containing serum on the apoptosis of chondrocytes induced by mechanics stimulus. Zhongguo Zhong Xi Yi Jie He Za Zhi. 32:789–792. 2012.(In Chinese). PubMed/NCBI

13 

Li X, Liang W, Dang C, et al: Empirical study on Bushen Zhuangjin Decoction inhibiting inflammatory cytokine expression experiments to delay the degeneration of articular cartilage. Feng Shi Bing Yu Guan Jie Yan. 3:20–25. 2014.(In Chinese).

14 

Cheng J, Li X, Ye H and Liu X: Review of the application of computer aided drug molecular docking design technology in filtering the effective components of Bushen Zhuangjin Decoction for prevention and cure of osteoarthritis. Zhong Yi Zheng Gu. 25:56–67. 2013.(In Chinese).

15 

Li H, Li X, Liu G, et al: Bauhinia championi (Benth.) Benth polysaccharides upregulate Wnt/β-catenin signaling in chondrocytes. Int J Mol Med. 32:1329–1336. 2013.PubMed/NCBI

16 

Yuan PW, Liu DY, Chu XD, Hao YQ, Zhu C and Qu Q: Effects of preventive administration of juanbi capsules on TNF-alpha, IL-1 and IL-6 contents of joint fluid in the rabbit with knee osteoarthritis. J Tradit Chin Med. 30:254–258. 2010. View Article : Google Scholar

17 

Li XH, Liang WN and Liu XX: Clinical observation on curative effect of dissolving phlegm-stasis on 50 cases of knee osteoarthritis. J Tradit Chin Med. 30:108–112. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Ohshika S, Ishibashi Y, Kon A, Kusumi T, Kijima H and Toh S: Potential of exogenous cartilage proteoglycan as a new material for cartilage regeneration. Int Orthop. 36:869–877. 2012. View Article : Google Scholar :

19 

Hoshiba T, Yamada T, Lu H, Kawazoe N and Chen G: Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion. J Biomed Mater Res A. 100:694–702. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Beier F: Cell-cycle control and the cartilage growth plate. J Cell Physiol. 202:1–8. 2005. View Article : Google Scholar

21 

Kamel S, Kruger C, Salbaum JM and Kappen C: Morpholino-mediated knockdown in primary chondrocytes implicates Hoxc8 in regulation of cell cycle progression. Bone. 44:708–716. 2009. View Article : Google Scholar :

22 

Löwenheim H, Reichl J, Winter H, et al: In vitro expansion of human nasoseptal chondrocytes reveals distinct expression profiles of G1 cell cycle inhibitors for replicative, quiescent, and senescent culture stages. Tissue Eng. 11:64–75. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Yu F, Li X, Cai L, et al: Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G1/S cell cycle transition. Mol Med Rep. 7:935–940. 2013.PubMed/NCBI

24 

Hwang SG, Song SM, Kim JR, Park CS, Song WK and Chun JS: Regulation of type II collagen expression by cyclin-dependent kinase 6, cyclin D1, and p21 in articular chondrocytes. IUBMB Life. 59:90–98. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Chen J, Liang W, Li H, Liu F, Weng X, Lin P, Chen W, Zheng C, Xu H, Xu H, et al: Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression. Exp Ther Med 9: 839-844, 2015.
APA
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X. ... Ye, H. (2015). Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression. Experimental and Therapeutic Medicine, 9, 839-844. https://doi.org/10.3892/etm.2015.2214
MLA
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X., Lin, P., Chen, W., Zheng, C., Xu, H., Liu, X., Ye, H."Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression". Experimental and Therapeutic Medicine 9.3 (2015): 839-844.
Chicago
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X., Lin, P., Chen, W., Zheng, C., Xu, H., Liu, X., Ye, H."Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression". Experimental and Therapeutic Medicine 9, no. 3 (2015): 839-844. https://doi.org/10.3892/etm.2015.2214
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Chen J, Liang W, Li H, Liu F, Weng X, Lin P, Chen W, Zheng C, Xu H, Xu H, et al: Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression. Exp Ther Med 9: 839-844, 2015.
APA
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X. ... Ye, H. (2015). Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression. Experimental and Therapeutic Medicine, 9, 839-844. https://doi.org/10.3892/etm.2015.2214
MLA
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X., Lin, P., Chen, W., Zheng, C., Xu, H., Liu, X., Ye, H."Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression". Experimental and Therapeutic Medicine 9.3 (2015): 839-844.
Chicago
Li, X., Chen, J., Liang, W., Li, H., Liu, F., Weng, X., Lin, P., Chen, W., Zheng, C., Xu, H., Liu, X., Ye, H."Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression". Experimental and Therapeutic Medicine 9, no. 3 (2015): 839-844. https://doi.org/10.3892/etm.2015.2214
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