High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts

  • Authors:
    • Xianglin Dong
    • Chuanshan Zhang
    • Shaolin Ma
    • Hao Wen
  • View Affiliations

  • Published online on: January 26, 2015     https://doi.org/10.3892/etm.2015.2216
  • Pages: 955-960
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Abstract

The aim of the present study was to investigate the effect of different concentrations of mast cell chymase on the transforming growth factor (TGF)‑β1/Smad signaling pathway in skin fibroblasts. Cultured skin fibroblasts were treated with various concentrations of chymase for different time periods. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay was used to assess the rate of cell proliferation. In addition, protein expression in the fibroblasts was measured using western blot analysis. Chymase was shown to enhance the proliferation of skin fibroblasts following incubation for 48, 72 and 96 h (P<0.01). Furthermore, high concentrations of mast cell chymase were shown to enhance the mRNA and protein expression levels of TGF‑β1 after long‑term (≥6 h) incubation. In addition, high concentrations of mast cell chymase increased P‑Smad2/3 and Smad2/3 protein expression. By contrast, low concentrations of mast cell chymase increased Smad7 protein expression. Therefore, the results demonstrated that high concentrations of mast cell chymase facilitated the transduction of the TGF‑β1/Smad signaling pathway in skin fibroblasts.
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March-2015
Volume 9 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Dong X, Zhang C, Ma S and Wen H: High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts. Exp Ther Med 9: 955-960, 2015
APA
Dong, X., Zhang, C., Ma, S., & Wen, H. (2015). High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts. Experimental and Therapeutic Medicine, 9, 955-960. https://doi.org/10.3892/etm.2015.2216
MLA
Dong, X., Zhang, C., Ma, S., Wen, H."High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts". Experimental and Therapeutic Medicine 9.3 (2015): 955-960.
Chicago
Dong, X., Zhang, C., Ma, S., Wen, H."High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts". Experimental and Therapeutic Medicine 9, no. 3 (2015): 955-960. https://doi.org/10.3892/etm.2015.2216