Effects of fentanyl, midazolam and their combination on immune function and mortality in mice with sepsis

Xiao,Dong; Zhang,Daquan; Xiang,Dongming; Liu,Qi; Liu,Yan; Lv,Lei; Xing,Xuezhong

doi:10.3892/etm.2015.2227

April-2015 Volume 9 Issue 4

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April-2015 Volume 9 Issue 4

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Article

Effects of fentanyl, midazolam and their combination on immune function and mortality in mice with sepsis

Authors:
- Dong Xiao
- Daquan Zhang
- Dongming Xiang
- Qi Liu
- Yan Liu
- Lei Lv
- Xuezhong Xing
View Affiliations / Copyright

Affiliations: Second Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur Autonomous Region, Ürümqi, Xinjiang 830001, P.R. China, Department of Critical Care Medicine, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China
Pages: 1494-1500
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Published online on: January 28, 2015

https://doi.org/10.3892/etm.2015.2227
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Abstract

The aim of this study was to investigate the effects of fentanyl and/or midazolam on the immune function and mortality of septic mice. Mice were randomly divided into sham‑operated, model, fentanyl‑, midazolam‑ and combination‑treated groups. The body weights and locomotor activities of the mice were measured, prior to and following surgery, and the mortality rates following surgery were recorded and compared among these groups. The organ weights and the corresponding coefficients were measured and calculated. Leukocyte numbers in peritoneal and thoracic cavity lavage fluid were counted, and the serum levels of the inflammation‑related cytokines interleukin (IL)‑1β, IL‑10, tumor necrosis factor (TNF)‑α, procalcitonin (PCT) and C‑reactive protein (CRP) were detected by enzyme‑linked immunosorbent assay (ELISA). The results demonstrated that the locomotor activities were reduced in septic mice, and medication led to significant declined body weights in these model animals. Importantly, the mortality rates of the septic mice were reduced by fentanyl and/or midazolam, and the histopathological changes were influenced by the medication. Moreover, in the medication‑treated groups, the leukocyte numbers in the peritoneal cavity lavage fluid were lower than those in the model group, while the medication slightly increased the numbers of leukocytes in the thoracic cavity lavage fluid. ELISA indicated that the levels of proinflammatory cytokines were reduced by fentanyl and/or midazolam, which may contribute to the beneficial effects of these medications in septic mice. Analgesic and/or sedative drugs could reduce inflammatory responses in septic mice, and immunosedation may have contributed to the improved mortality rates in these models. These results provide a theoretical basis for further clinical studies concerning the treatment of sepsis with these medications.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of fentanyl, midazolam and their combination on immune function and mortality in mice with sepsis

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