Study of genes associated with the ‘anger-in’ and ‘anger-out’ emotions of humans using a rat model

Guo,Yinghui; Zhang,Huiyun; Gao,Jie; Wei,Sheng; Song,Chunhong; Sun,Peng; Qiao,Mingqi

doi:10.3892/etm.2015.2246

Study of genes associated with the ‘anger-in’ and ‘anger-out’ emotions of humans using a rat model

Authors:
- Yinghui Guo
- Huiyun Zhang
- Jie Gao
- Sheng Wei
- Chunhong Song
- Peng Sun
- Mingqi Qiao
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Affiliations: College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, P.R. China, Key Laboratory of Classic Theories on Traditional Chinese Medicine, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, P.R. China
Published online on: February 2, 2015 https://doi.org/10.3892/etm.2015.2246
Pages: 1448-1454

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Abstract

The aim of the present study was to investigate the genes associated with ‘anger‑in’ (tendency to suppress anger) and ‘anger‑out’ (tendency to express anger through verbal or physical means) emotions in humans. Wistar rats were divided into five groups (n=10/group), based on the type of model and the Chinese medicinal formulation administered, and the rat models were established. The five groups were as follows: Normal control (control), anger‑in model (AIM), anger‑in Jingqianshu‑administered (AIA), anger‑out model (AOM) and anger‑out Jingqianping‑administered (AOA). Open‑field, resident‑intruder and aggressive behavior tests were carried out, as well as gene expression analysis, reverse transcription‑quantitative polymerase chain reaction and western blot analyses. The body weights of the rats in the AIM and AOM groups were significantly lower than those of the control group rats. The open‑field test indicated that the scores in the AOM group were significantly higher (P<0.05) than those in the AIM group. The aggression scores of the rats in the AOM group were significantly higher than those of the AIM group rats. Jingqianshu and Jingqianping granules attenuated the behavioral changes of the rats. 5‑Htr2C, GABABR2 and 5‑Htr3B were associated with anger‑in and anger‑out emotions. Jingqianping and Jingqianshu granules attenuated the changes in the mRNA expression of 5‑Htr2C, GABABR2 and 5‑Htr3B, as indicated by RT‑qPCR, and showed similar effects on protein expression, as demonstrated by western blot analysis. The present study demonstrated that the anger‑in and anger‑out emotions of rats are closely associated with 5‑Htr2C, GABABR2 and 5‑Htr3B genes, and that Jingqianshu and Jingqianping granules attenuate the abnormal behaviors of model rats. These findings may be useful for the treatment of emotional disorders associated with anger.

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