Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats

  • Authors:
    • Jie Guo
    • Lue Wang
    • Haihua Xu
    • Xiaoxia Che
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China, Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, P.R. China
  • Pages: 1235-1240
    |
    Published online on: February 3, 2015
       https://doi.org/10.3892/etm.2015.2253
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to explore whether bone marrow mononuclear cell (BMMC) transplantation is able to accelerate the bone remodeling induced by midpalatal expansion in rats. A total of 48 male Sprague‑Dawley rats (mean weight, 208.36±7.32 g) were divided into control and midpalatal expansion with or without BMMC transplantation groups. Histological and morphological changes were observed in each group. The osteogenic activities and differential potentials of the transplanted BMMCs labeled with bromodeoxyuridine in the midpalatal bone tissue were assessed by osteocalcin expression. The receptor activator of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) ratio was determined by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to reflect the equilibrium between bone resorption and formation. The results demonstrated that the width of the maxillary dental arch increased distinctly within 2 weeks of midpalatal expansion with BMMC transplantation. The morphology of the midpalatal suture in this group changed significantly; the cartilage was completely replaced by fibrous‑like tissue expressing osteocalcin. The palatal bone was reorganized from a cancellous form into a mature compact structure after an additional 2‑week relapse period. Immunostaining results indicated that the heterologous transplanted BMMCs survived and differentiated into osteoblasts during the remodeling induced by midpalatal expansion. The RANKL/OPG expression ratio significantly decreased after 2 weeks of midpalatal expansion with BMMC transplantation due to the inhibition of RANKL expression. Heterologous BMMC transplantation appears to accelerate the midpalatal bone remodeling induced by expansion of the rats through increasing the number of osteoprogenitor cells and regulating the RANKL‑OPG signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

de Gurgel JA, Malmström MF and Pinzan-Vercelino CR: Ossification of the midpalatal suture after surgically assisted rapid maxillary expansion. Eur J Orthod. 34:39–43. 2012. View Article : Google Scholar

2 

Ahrari F and Eslami N: Nonsurgical treatment of maxillary deficiency using tongue guard appliance: a case report. J Dent Res Dent Clin Dent Prospects. 5:136–140. 2011.

3 

Verstraaten J, Kuijpers-Jagtman AM, Mommaerts MY, et al: A systematic review of the effects of bone-borne surgical assisted rapid maxillary expansion. J Craniomaxillofac Surg. 38:166–174. 2010. View Article : Google Scholar

4 

Petrick S, Hothan T, Hietschold V, et al: Bone density of the midpalatal suture 7 months after surgically assisted rapid palatal expansion in adults. Am J Orthod Dentofacial Orthop. 139(4 Suppl): S109–S116. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Wertz RA: Skeletal and dental changes accompanying rapid midpalatal suture opening. Am J Orthod. 58:41–66. 1970. View Article : Google Scholar : PubMed/NCBI

6 

Chung CH and Font B: Skeletal and dental changes in the sagittal, vertical, and transverse dimensions after rapid palatal expansion. Am J Orthod Dentofacial Orthop. 126:569–575. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Lines PA: Adult rapid maxillary expansion with corticotomy. Am J Orthod. 67:44–56. 1975. View Article : Google Scholar : PubMed/NCBI

8 

Lagravere MO, Major PW and Flores-Mir C: Long-term dental arch changes after rapid maxillary expansion treatment: a systematic review. Angle Orthod. 75:155–161. 2005.PubMed/NCBI

9 

Vardimon AD, Brosh T, Spiegler A, Lieberman M and Pitaru S: Rapid palatal expansion: Part 1. Mineralization pattern of the midpalatal suture in cats. Am J Orthod Dentofacial Orthop. 113:371–378. 1998.PubMed/NCBI

10 

Bianchi A, Amadori S, Pironi M and Marchetti C: Maxillary expansion and stability in the orthodontic-surgical treatment of skeletal anterior open bites. Prog Orthod. 10:26–37. 2009.PubMed/NCBI

11 

Oztürk F, Babacan H, Inan S and Gümüş C: Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study. Am J Orthod Dentofacial Orthop. 140:e31–e41. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Sawada M and Shimizu N: Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-beta 1 in the rat. Eur J Orthod. 18:169–179. 1996.PubMed/NCBI

13 

Khosla S, Westendorf JJ and Mödder UI: Concise review: Insights from normal bone remodeling and stem cell-based therapies for bone repair. Stem Cells. 28:2124–2128. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Li Z, Liao W, Zhao Q, et al: Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 183:193–203. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Li F, Wang X and Niyibizi C: Bone marrow stromal cells contribute to bone formation following infusion into femoral cavities of a mouse model of osteogenesis imperfecta. Bone. 47:546–555. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Quarto R, Thomas D and Liang CT: Bone progenitor cell deficits and the age-associated decline in bone repair capacity. Calcif Tissue Int. 56:123–129. 1995. View Article : Google Scholar : PubMed/NCBI

17 

Liang CT, Barnes J, Seedor JG, et al: Impaired bone activity in aged rats: alterations at the cellular and molecular levels. Bone. 13:435–441. 1992. View Article : Google Scholar : PubMed/NCBI

18 

Lee K, Sugiyama H, Imoto S and Tanne K: Effects of bisphosphonate on the remodeling of rat sagittal suture after rapid expansion. Angle Orthod. 71:265–273. 2001.PubMed/NCBI

19 

Kobayashi ET, Hashimoto F, Kobayashi Y, et al: Force-induced rapid changes in cell fate at midpalatal suture cartilage of growing rats. J Dent Res. 78:1495–1504. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Pérez-Sayáns M, Somoza-Martin JM, Barros-Angueira F, Rey JM and García-García A: RANK/RANKL/OPG role in distraction osteogenesis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 109:679–686. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Hernigou P, Poignard A, Beaujean F and Rouard H: Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am. 87:1430–1437. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Ma JT, Yu M, Zhang MC, et al: Clinical observation on percutaneous autologous bone marrow grafting for treatment of fracture nonunion. Zhongguo Gu Shang. 22:862–864. 2009.(In Chinese).

23 

Hatzokos I, Stavridis SI, Iosifidou E, Karataglis D and Christodoulou A: Autologous bone marrow grafting combined with demineralized bone matrix improves consolidation of docking site after distraction osteogenesis. J Bone Joint Surg Am. 93:671–678. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Yao S, McCarthy PL, Dunford LM, et al: High prevalence of early-onset osteopenia/osteoporosis after allogeneic stem cell transplantation and improvement after bisphosphonate therapy. Bone Marrow Transplant. 41:393–398. 2008. View Article : Google Scholar

25 

No authors listed. The treatment of osteoporosis through transplantation of bone marrow stem cells in the experiments performed on rats. Georgian Med News. 204:88–92. 2012.PubMed/NCBI

26 

Trouvin AP and Goëb V: Receptor activator of nuclear factor-kappaB ligand and osteoprotegerin: maintaining the balance to prevent bone loss. Clin Interv Aging. 5:345–354. 2010.

27 

Kon T, Cho TJ, Aizawa T, et al: Expression of osteoprotegerin, receptor activator of NF-kappaB ligand (osteoprotegerin ligand) and related proinflammatory cytokines during fracture healing. J Bone Miner Res. 16:1004–1014. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Ricci P, Tauchmanova L, Risitano AM, et al: Imbalance of the osteoprotegerin/RANKL ratio in bone marrow microenvironment after allogeneic hemopoietic stem cell transplantation. Transplantation. 82:1449–1456. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Zhu WQ, Wang X, Wang XX and Wang ZY: Temporal and spatial expression of osteoprotegerin and receptor activator of nuclear factor -kappaB ligand during mandibular distraction in rats. J Craniomaxillofac Surg. 35:103–111. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo J, Wang L, Xu H and Che X: Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats. Exp Ther Med 9: 1235-1240, 2015.
APA
Guo, J., Wang, L., Xu, H., & Che, X. (2015). Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats. Experimental and Therapeutic Medicine, 9, 1235-1240. https://doi.org/10.3892/etm.2015.2253
MLA
Guo, J., Wang, L., Xu, H., Che, X."Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats". Experimental and Therapeutic Medicine 9.4 (2015): 1235-1240.
Chicago
Guo, J., Wang, L., Xu, H., Che, X."Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1235-1240. https://doi.org/10.3892/etm.2015.2253
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Wang L, Xu H and Che X: Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats. Exp Ther Med 9: 1235-1240, 2015.
APA
Guo, J., Wang, L., Xu, H., & Che, X. (2015). Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats. Experimental and Therapeutic Medicine, 9, 1235-1240. https://doi.org/10.3892/etm.2015.2253
MLA
Guo, J., Wang, L., Xu, H., Che, X."Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats". Experimental and Therapeutic Medicine 9.4 (2015): 1235-1240.
Chicago
Guo, J., Wang, L., Xu, H., Che, X."Effect of heterologous bone marrow mononuclear cell transplantation on midpalatal expansion in rats". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1235-1240. https://doi.org/10.3892/etm.2015.2253
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team