Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold

Sun,Anke; Meng,Qingyan; Li,Wantong; Liu,Songbo; Chen,Wei

doi:10.3892/etm.2015.2262

Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold

Authors:
- Anke Sun
- Qingyan Meng
- Wantong Li
- Songbo Liu
- Wei Chen
View Affiliations

Affiliations: Department of Otolaryngology, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China, Department of Burns and Aesthetic Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China, Department of Microsurgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China, Department of Experimental Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China
Published online on: February 5, 2015 https://doi.org/10.3892/etm.2015.2262
Pages: 1482-1488

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to construct tissue‑engineered laryngeal cartilage with a hollow, semi‑flared shape using a poly(3‑hydroxybutyrate‑co‑3‑hydroxyhexanoate) (PHBHH) scaffold. Porous PHBHH was prepared and formed into a hollow, semi‑flared shape, and the cell‑material composites were cultured for one week in vitro prior to implantation in vivo. Cells of the nine rabbits of the experimental group were filled and encapsulated in the myofascial flap‑shaping material composite for in situ implantation. The three rabbits in the control group were treated with the shaping material without the chondrocytes. Cartilage regeneration was assessed at six, 12 and 18 weeks after surgery. In the experimental group, the shape and porosity of the material were ideal, the cells exhibited good adhesion with the material and the myofascial flap blood supply was rich. The engineered laryngeal cartilage with the hollow, semi‑flared shape was ideally formed, and the cartilage formed at six weeks after the surgery. Further maturation of the cartilage was observed at 12 and 18 weeks after the surgery. PHBHH was a suitable material for the formation of a hollow, semi‑flared shape with good cellular compatibility. Myofascial flap filling and wrapping can be used to build tissue‑engineered laryngeal cartilage with a hollow, semi‑flared shape.

View Figures

View References

1	Sterodimas A and de Faria J: Human auricular tissue engineering in an immunocompetent animal model. Aesthet Surg J. 33:283–289. 2013. View Article : Google Scholar : PubMed/NCBI
2	Cheng NC, Estes BT, Young TH and Guilak F: Engineered cartilage using primary chondrocytes cultured in a porous cartilage-derived matrix. Regen Med. 6:81–93. 2011. View Article : Google Scholar :
3	Fishman JM, De Coppi P, Elliott MJ, Atala A, Birchall MA and Macchiarini P: Airway tissue engineering. Expert Opin Biol Ther. 11:1623–1635. 2011. View Article : Google Scholar : PubMed/NCBI
4	Fisher MB and Mauck RL: Tissue engineering and regenerative medicine: recent innovations and the transition to translation. Tissue Eng Part B Rev. 19:1–13. 2013. View Article : Google Scholar :
5	Lu T, Li Y and Chen T: Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering. Int J Nanomedicine. 8:337–350. 2013. View Article : Google Scholar : PubMed/NCBI
6	Deng Y, Lin XS, Zheng Z, Deng JG, Chen JC, Ma H and Chen GQ: Poly(hydroxybutyrate-co-hydroxyhexanoate) promoted production of extracellular matrix of articular cartilage chondrocytes in vitro. Biomaterials. 24:4273–4281. 2003. View Article : Google Scholar : PubMed/NCBI
7	Veleirinho B, Coelho DS, Dias PF, Maraschin M, Ribeiro-do-Valle RM and Lopes-da-Silva JA: Nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/chitosan scaffolds for skin regeneration. Int J Biol Macromol. 51:343–350. 2012. View Article : Google Scholar : PubMed/NCBI
8	Mikos AG, Sarakinos G, Leite SM, Vacanti JP and Langer R: Laminated three-dimensional biodegradable foams for use in tissue engineering. Biomaterials. 14:323–330. 1993. View Article : Google Scholar : PubMed/NCBI
9	Zhang R and Ma PX: Poly(alpha-hydroxylacids)/hydroxyaptite porous composites for bone-tissue engineering. I Preparation and morphology. J Biomed Mater Res. 44:446–455. 1999. View Article : Google Scholar : PubMed/NCBI
10	Gilpin DA, Weidenbecher MS and Dennis JE: Scaffold-free tissue-engineered cartilage implants for laryngotracheal reconstruction. Laryngoscope. 120:612–617. 2010. View Article : Google Scholar : PubMed/NCBI
11	Shahin K and Doran PM: Improved seeding of chondrocytes into polyglycolic acid scaffolds using semi-static and alginate loading methods. Biotechnol Prog. 27:191–200. 2011. View Article : Google Scholar : PubMed/NCBI
12	El Sayed K, Marzahn U, John T, et al: PGA-associated heterotopic chondrocyte cocultures: implications of nasoseptal and auricular chondrocytes in articular cartilage repair. J Tissue Eng Regen Med. 7:61–72. 2013. View Article : Google Scholar
13	Chaim IA, Sabino MA, Mendt M, Müller AJ and Ajami D: Evaluation of the potential of novel PCL-PPDX biodegradable scaffolds as support materials for cartilage tissue engineering. J Tissue Eng Regen Med. 6:272–279. 2012. View Article : Google Scholar
14	Xue J, Feng B, Zheng R, et al: Engineering ear-shaped cartilage using electrospun fibrous membranes of gelatin/polycaprolactone. Biomaterials. 34:2624–2631. 2013. View Article : Google Scholar : PubMed/NCBI
15	Vacanti CA and Upton J: Tissue-engineered morphogenesis of cartilage and bone by means of cell transplantation using synthetic biodegradable polymer matrices. Clin Plast Surg. 21:445–462. 1994.PubMed/NCBI
16	Cohen SB, Meirisch CM, Wilson HA and Diduch DR: The use of absorbable co-polymer pads with alginate and cells for articular cartilage repair in rabbits. Biomaterials. 24:2653–2660. 2003. View Article : Google Scholar : PubMed/NCBI
17	Baek CH and Ko YJ: Characteristics of tissue-engineered cartilage on macroporous biodegradable PLGA scaffold. Laryngoscope. 116:1829–1834. 2006. View Article : Google Scholar : PubMed/NCBI
18	Zhijun L and Wanming W: Latest progress in biological scaffold materials for tissue engineered cartilage. Zhongguo zu zhi gong cheng yan jiu. 11:3625–3628. 2007.
19	Asti A and Gioglio L: Natural and synthetic biodegradable polymers: different scaffolds for cell expansion and tissue formation. Int J Artif Organs. 37:187–205. 2014.PubMed/NCBI
20	Zhao K, Deng Y, Chun Chen J and Chen GQ: Polyhydroxyalkanoate (PHA) scaffolds with good mechanical properties and biocompatibility. Biomaterials. 24:1041–1045. 2003. View Article : Google Scholar
21	Ye C, Hu P, Ma MX, Xiang Y, Liu RG and Shang XW: PHB/PHBHHX scaffolds and human adipose-derived stem cells for cartilage tissue engineering. Biomaterials. 30:4401–4406. 2009. View Article : Google Scholar : PubMed/NCBI
22	Sun AK, Li WT, Meng QY, Liu SB, Chen W and Tang WW: Fabrication of larynx-shape tissue engineered cartilage by means of filling together with wrapping with pedicle myofascial flap. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 46:1019–1023. 2011.(In Chinese).
23	Nomoto Y, Okano W, Imaizumi M, Tani A, Nomoto M and Omori K: Bioengineered prosthesis with allogenic heterotopic fibroblasts for cricoid regeneration. Laryngoscope. 122:805–809. 2012. View Article : Google Scholar : PubMed/NCBI
24	Lange P, Fishman JM, Elliott MJ, De Coppi P and Birchall MA: What can regenerative medicine offer for infants with laryngotracheal agenesis? Otolaryngol Head Neck Surg. 145:544–550. 2011. View Article : Google Scholar : PubMed/NCBI
25	Kanemaru S, Hirano S, Umeda H, et al: A tissue-engineering approach for stenosis of the trachea and/or cricoid. Acta Otolaryngol Suppl. 563:79–83. 2010. View Article : Google Scholar : PubMed/NCBI
26	Glatz F, Neumeister M, Suchy H, Lyons S, Damikas D and Mowlavi A: A tissue-engineering technique for vascularized laryngotracheal reconstruction. Arch Otolaryngol Head Neck Surg. 129:201–206. 2003. View Article : Google Scholar : PubMed/NCBI
27	Aslim EJ, Rasheed MZ, Lin F, Ong YS and Tan BK: Use of the anterolateral thigh and vertical rectus abdominis musculocutaneous flaps as utility flaps in reconstructing large groin defects. Arch Plast Surg. 41:556–561. 2014. View Article : Google Scholar : PubMed/NCBI
28	Fang SL, Wang YY, Chen WL and Zhang DM: Use of extended vertical lower trapezius island myocutaneous flaps to cover exposed reconstructive plates. J Oral Maxillofac Surg. 72:209.e1–7. 2014. View Article : Google Scholar
29	Zhi L, Wenli W, Pengfei G, et al: Laryngotracheal reconstruction with autogenous rib cartilage graft for complex laryngotracheal stenosis and/or anterior neck defect. Eur Arch Otorhinolaryngol. 271:317–322. 2014. View Article : Google Scholar
30	Chanowski EJ, Haxer MJ and Chepeha DB: Microvascular cricoid cartilage reconstruction with the thoracodorsal artery scapular tip autogenous transplant. Laryngoscope. 122:282–285. 2012. View Article : Google Scholar
31	Zhao Y, Zhang W, Zhao J, Li Z and He S: Reconstruction of soft tissue defects in oral and maxillofacial regions after tumors surgery using cervical pedicle tissue flaps. Zhogguo Xiu Fu Chong Jian Wai Ke Za Zhi. 19:780–783. 2005.