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Article

Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro

  • Authors:
    • Shang Gao
    • Yuzhuo Wang
    • Xiaolong Wang
    • Peng Lin
    • Min Hu
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1277-1282
    |
    Published online on: February 9, 2015
       https://doi.org/10.3892/etm.2015.2276
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Abstract

The applications of lithium ions as an agent to facilitate bone formation have been widely documented; however, the effect of lithium ions in the periodontitis model has not yet been elucidated. The aim of the present study, therefore, was to investigate the effect of single lithium ions in the presence of lipopolysaccharide (LPS). A periodontitis model was induced in cementoblasts using LPS. The cytotoxic effect of the lithium ions on the cementoblasts was studied through the MTT assay. Alkaline phosphatase analysis and alizarin red staining were performed to investigate the effect of the lithium ions on differentiation. To examine the effect of lithium ions on osteoclastogenesis, osteoprotegerin (OPG) and receptor activator of nuclear factor‑κB ligand (RANKL) mRNA and protein expression levels were assessed using reverse transcription‑polymerase chain reaction analysis and ELISA, respectively. Compared with the effect induced by lithium ions on normal cementoblasts, proliferation and differentiation were downregulated following the co‑incubation of the cementoblasts with LPS and lithium ions. Furthermore, the lithium ions appeared to alter osteoclastogenesis by regulating the OPG/RANKL ratio. In conclusion, the present findings suggest that lithium ions can downregulate proliferation and differentiation in a periodontitis model. Further studies should be undertaken prior to the acceptance of lithium ions for use in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Gao S, Wang Y, Wang X, Lin P and Hu M: Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro. Exp Ther Med 9: 1277-1282, 2015.
APA
Gao, S., Wang, Y., Wang, X., Lin, P., & Hu, M. (2015). Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro. Experimental and Therapeutic Medicine, 9, 1277-1282. https://doi.org/10.3892/etm.2015.2276
MLA
Gao, S., Wang, Y., Wang, X., Lin, P., Hu, M."Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro". Experimental and Therapeutic Medicine 9.4 (2015): 1277-1282.
Chicago
Gao, S., Wang, Y., Wang, X., Lin, P., Hu, M."Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1277-1282. https://doi.org/10.3892/etm.2015.2276
Copy and paste a formatted citation
x
Spandidos Publications style
Gao S, Wang Y, Wang X, Lin P and Hu M: Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro. Exp Ther Med 9: 1277-1282, 2015.
APA
Gao, S., Wang, Y., Wang, X., Lin, P., & Hu, M. (2015). Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro. Experimental and Therapeutic Medicine, 9, 1277-1282. https://doi.org/10.3892/etm.2015.2276
MLA
Gao, S., Wang, Y., Wang, X., Lin, P., Hu, M."Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro". Experimental and Therapeutic Medicine 9.4 (2015): 1277-1282.
Chicago
Gao, S., Wang, Y., Wang, X., Lin, P., Hu, M."Effect of lithium ions on cementoblasts in the presence of lipopolysaccharide in vitro". Experimental and Therapeutic Medicine 9, no. 4 (2015): 1277-1282. https://doi.org/10.3892/etm.2015.2276
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