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Article

Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis

  • Authors:
    • Guicun Xu
    • Mingming Xue
    • Haiyan Wang
    • Chun Xiang
  • View Affiliations / Copyright

    Affiliations: Emergency Department, First Affiliated Hospital of Inner Mongolia Medical University, Hohot, Inner Mongolia 010059, P.R. China, Laboratory of Physiology, College of Basic Medicine, Inner Mongolia Medical University, Hohot, Inner Mongolia 010110, P.R. China, Department of Anatomy, College of Basic Medicine, Inner Mongolia Medical University, Hohot, Inner Mongolia 010110, P.R. China
  • Pages: 1801-1806
    |
    Published online on: March 2, 2015
       https://doi.org/10.3892/etm.2015.2319
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Abstract

Bone fracture is accompanied with poor oxygen supply and nutrient deficiency in the local fracture site, and oxygen supply is an important factor that can affect fracture healing. Hypoxia‑inducible factor‑1 (HIF‑1) plays a key role in the regulation of oxygen homeostasis. HIF‑1α is rapidly upregulated in response to hypoxia and antagonizes hypoxia‑induced apoptosis. In the present study, the viability of an osteoblast cell line, MC3T3‑E1, and the expression of HIF‑1α protein in the MC3T3‑E1 cells was examined under hypoxic conditions. The HIF‑1α level was then manipulated and the reduction in the viability of the MC3T3‑E1 cells in response to the hypoxia was re‑evaluated. In addition, the regulation of HIF‑1α in the adaptation of MC3T3‑E1 cells to hypoxia was explored. The results showed that the viability of MC3T3‑E1 cells decreased and the expression of HIF‑1α protein increased under hypoxic conditions. Furthermore, the reduction in the viability of MC3T3‑E1 cells post‑hypoxia was attenuated by HIF‑1α overexpression, while HIF‑1α‑knockdown by small interfering RNA enhanced the hypoxia‑induced decrease in cell viability. It was additionally found that the forced expression of HIF‑1α inhibited the hypoxia‑induced cell apoptosis. These findings indicate that the forced expression of HIF‑1α inhibits hypoxia‑induced apoptosis and thus attenuates the hypoxia‑induced decrease in cell viability.
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Copy and paste a formatted citation
Spandidos Publications style
Xu G, Xue M, Wang H and Xiang C: Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis. Exp Ther Med 9: 1801-1806, 2015.
APA
Xu, G., Xue, M., Wang, H., & Xiang, C. (2015). Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis. Experimental and Therapeutic Medicine, 9, 1801-1806. https://doi.org/10.3892/etm.2015.2319
MLA
Xu, G., Xue, M., Wang, H., Xiang, C."Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis". Experimental and Therapeutic Medicine 9.5 (2015): 1801-1806.
Chicago
Xu, G., Xue, M., Wang, H., Xiang, C."Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1801-1806. https://doi.org/10.3892/etm.2015.2319
Copy and paste a formatted citation
x
Spandidos Publications style
Xu G, Xue M, Wang H and Xiang C: Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis. Exp Ther Med 9: 1801-1806, 2015.
APA
Xu, G., Xue, M., Wang, H., & Xiang, C. (2015). Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis. Experimental and Therapeutic Medicine, 9, 1801-1806. https://doi.org/10.3892/etm.2015.2319
MLA
Xu, G., Xue, M., Wang, H., Xiang, C."Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis". Experimental and Therapeutic Medicine 9.5 (2015): 1801-1806.
Chicago
Xu, G., Xue, M., Wang, H., Xiang, C."Hypoxia‑inducible factor‑1α antagonizes the hypoxia‑mediated osteoblast cell viability reduction by inhibiting apoptosis". Experimental and Therapeutic Medicine 9, no. 5 (2015): 1801-1806. https://doi.org/10.3892/etm.2015.2319
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