Chebulagic acid inhibits the LPS‑induced expression of TNF‑α and IL‑1β in endothelial cells by suppressing MAPK activation

Liu,Yueying; Bao,Luer; Xuan,Liying; Song,Baohua; Lin,Lin; Han,Hao

doi:10.3892/etm.2015.2447

Chebulagic acid inhibits the LPS‑induced expression of TNF‑α and IL‑1β in endothelial cells by suppressing MAPK activation

Authors:
- Yueying Liu
- Luer Bao
- Liying Xuan
- Baohua Song
- Lin Lin
- Hao Han
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Affiliations: Teaching and Research Section of Physiology and Pathophysiology, Medical College, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028041, P.R. China
Published online on: April 23, 2015 https://doi.org/10.3892/etm.2015.2447
Pages: 263-268

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Abstract

Inflammatory response in the vasculature, including the overexpression of tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β, has been demonstrated to increase the risk of thrombosis development. Chebulagic acid (CA) is a key chemical component in the traditional Mongolian anti‑thrombotic drug Garidi‑13, and has been suggested to exert anti‑inflammatory and anti‑infective effects. The present study aimed to evaluate the regulatory impact of CA on a number of biological processes, including lipopolysaccharide (LPS)‑induced inflammation, LPS‑promoted mitogen‑activated protein kinase (MAPK) activation and the expression of toll‑like receptor (TLR)4 in EA.hy926 human endothelial cells. The results indicated that CA significantly inhibited the LPS‑induced upregulation of TNF‑α and IL‑1β in a dose‑ and time‑dependent manner. Furthermore, LPS‑activated MAPK signaling was inhibited by CA treatment in the EA.hy926 cells. However, TLR4, which serves a key function in LPS‑induced inflammation as the receptor of LPS, was not regulated by the CA treatment. In summary, the results of the present study indicate that CA inhibits the LPS‑induced promotion of TNF‑α and IL‑1β in endothelial cells by suppressing MAPK activation, which may contribute to the anti‑thrombotic effect of Garidi‑13.

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