Hair follicle units promote re-epithelialization in chronic cutaneous wounds: A clinical case series study

Liu,Jia‑Qi; Zhao,Kong-Bo; Feng,Zi‑Hao; Qi,Fa‑Zhi

doi:10.3892/etm.2015.2465

Hair follicle units promote re-epithelialization in chronic cutaneous wounds: A clinical case series study

Authors:
- Jia‑Qi Liu
- Kong-Bo Zhao
- Zi‑Hao Feng
- Fa‑Zhi Qi
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Affiliations: Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Surgery, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China
Published online on: April 30, 2015 https://doi.org/10.3892/etm.2015.2465
Pages: 25-30
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic cutaneous wounds are one of the most unfavorable pathophysiological processes in routine practice. However, developments in hair follicle unit therapy may aid the treatment of these wounds. The aim of the present study was to investigate the function of hair follicle units in chronic cutaneous wound re‑epithelialization and to develop an effective protocol for wound treatment. A total of 14 patients, of which nine were male and five were female, with a mean age of 60.71 years (range, 19‑76 years) and a mean wound area of 74.14 cm2, were treated in the study. The hair follicle units were dissected from a scalp graft and transplanted into the chronic cutaneous wound bed, after which clinical evaluation was performed. Images of the recipient site were captured at 0, 1, 2, 3, 4, 5, 8 and 14 weeks following transplantation. In addition, histological examinations were conducted postoperatively at week 16. Total wound re‑epithelialization was observed in all the patients. Histological analysis revealed that the epidermis and papillary dermis were present in the wound area. Adnexal structures and the reticular dermis were also observed. Therefore, the present study demonstrated the ability of hair follicle units to promote chronic cutaneous wound healing.

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1	Castilla DM, Liu ZJ, Tian R, Li Y, Livingstone AS and Velazquez OC: A novel autologous cell-based therapy to promote diabetic wound healing. Ann Surg. 256:560–572. 2012. View Article : Google Scholar : PubMed/NCBI
2	Lau K, Paus R, Tiede S, Day P and Bayat A: Exploring the role of stem cells in cutaneous wound healing. Exp Dermatol. 18:921–933. 2009. View Article : Google Scholar : PubMed/NCBI
3	Hu L, Zhao J, Liu J, Gong N and Chen L: Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes. Exp Ther Med. 5:701–706. 2013.PubMed/NCBI
4	Dittmer J and Leyh B: Paracrine effects of stem cells in wound healing and cancer progression (Review). Int J Oncol. 44:1789–1798. 2014.PubMed/NCBI
5	Brower J, Blumberg S, Carroll E, Pastar I, Brem H and Chen W: Mesenchymal stem cell therapy and delivery systems in nonhealing wounds. Adv Skin Wound Care. 24:524–534. 2011. View Article : Google Scholar : PubMed/NCBI
6	Jahoda CA and Christiano AM: Niche crosstalk: Intercellular signals at the hair follicle. Cell. 146:678–681. 2011. View Article : Google Scholar : PubMed/NCBI
7	Murawala P, Tanaka EM and Currie JD: Regeneration: The ultimate example of wound healing. Semin Cell Dev Biol. 23:954–962. 2012. View Article : Google Scholar : PubMed/NCBI
8	Waters JM, Richardson GD and Jahoda CA: Hair follicle stem cells. Semin Cell Dev Biol. 18:245–254. 2007. View Article : Google Scholar : PubMed/NCBI
9	Mimoun M, Chaouat M, Picovski D, Serroussi D and Smarrito S: The scalp is an advantageous donor site for thin-skin grafts: A report on 945 harvested samples. Plast Reconstr Surg. 118:369–373. 2006. View Article : Google Scholar : PubMed/NCBI
10	Zakine G, Mimoun M, Pham J and Chaouat M: Reepithelialization from stem cells of hair follicles of dermal graft of the scalp in acute treatment of third-degree burns: first clinical and histologic study. Plast Reconstr Surg. 130:42e–50e. 2012. View Article : Google Scholar : PubMed/NCBI
11	Navsaria HA, Ojeh NO, Moiemen N, Griffiths MA and Frame JD: Reepithelialization of a full-thickness burn from stem cells of hair follicles micrografted into a tissue-engineered dermal template (Integra). Plast Reconstr Surg. 113:978–981. 2004. View Article : Google Scholar : PubMed/NCBI
12	Plikus MV, Gay DL, Treffeisen E, Wang A, Supapannachart RJ and Cotsarelis G: Epithelial stem cells and implications for wound repair. Semin Cell Dev Biol. 23:946–953. 2012. View Article : Google Scholar : PubMed/NCBI
13	Skardal A, Mack D, Kapetanovic E, Atala A, Jackson JD, Yoo J and Soker S: Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med. 1:792–802. 2012. View Article : Google Scholar : PubMed/NCBI
14	Brown JB and McDowell F: Epithelial healing and the transplantation of skin. Ann Surg. 115:1166–1181. 1942. View Article : Google Scholar : PubMed/NCBI
15	Jaks V, Kasper M and Toftgård R: The hair follicle - a stem cell zoo. Exp Cell Res. 316:1422–1428. 2010. View Article : Google Scholar : PubMed/NCBI
16	Jiménez F, Garde C, Poblet E, et al: A pilot clinical study of hair grafting in chronic leg ulcers. Wound Repair Regen. 20:806–814. 2012. View Article : Google Scholar : PubMed/NCBI
17	Jahoda CA, Horne KA and Oliver RF: Induction of hair growth by implantation of cultured dermal papilla cells. Nature. 311:560–562. 1984. View Article : Google Scholar : PubMed/NCBI
18	Hill RP, Gardner A, Crawford HC, et al: Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture. Exp Dermatol. 22:236–238. 2013. View Article : Google Scholar : PubMed/NCBI
19	Jensen KB, Collins CA, Nascimento E, Tan DW, Frye M, Itami S and Watt FM: Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis. Cell Stem Cell. 4:427–439. 2009. View Article : Google Scholar : PubMed/NCBI
20	Snippert HJ, Haegebarth A, Kasper M, et al: Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin. Science. 327:1385–1389. 2010. View Article : Google Scholar : PubMed/NCBI
21	Jahoda CA and Reynolds AJ: Hair follicle dermal sheath cells: unsung participants in wound healing. Lancet. 358:1445–1448. 2001. View Article : Google Scholar : PubMed/NCBI
22	Leirós GJ, Kusinsky AG, Drago H, et al: Dermal papilla cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using hair follicle stem cells. Stem Cells Transl Med. 3:1209–1219. 2014. View Article : Google Scholar : PubMed/NCBI
23	Baquerizo Nole KL and Kirsner RS: Hair follicles and their potential in wound healing. Exp Dermatol. 24:95–96. 2015. View Article : Google Scholar : PubMed/NCBI