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Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways

  • Authors:
    • Weiqi Yao
    • Qinyong Hu
    • Yuhong Ma
    • Wenping Xiong
    • Tingting Wu
    • Jun Cao
    • Dongcheng Wu
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Chemistry, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Oncology, Ren Ming Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 468-476
    |
    Published online on: May 21, 2015
       https://doi.org/10.3892/etm.2015.2505
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Abstract

Cisplatin has been hypothesized to induce nephrotoxicity through triggering the apoptosis of tubular cells; however, the drug remains widely administered for the treatment of tumors. Recently, mesenchymal stem cells (MSCs) have been demonstrated to protect the kidney from the adverse effects induced by cisplatin. The aim of the present study was to investigate the mechanisms underlying the protective effects of human adipose‑derived MSCs (AD‑MSCs) on kidney function and tubular cells. Sprague‑Dawley rats were divided into three groups, which included the healthy controls, those subjected to cisplatin‑induced acute kidney injury (AKI) for 24 h without subsequent treatment and those subjected to cisplatin‑induced AKI for 24 h, followed by AD‑MSC engraftment. The rats were sacrificed at day 5 and the effects were analyzed using various methods, including biochemical analysis, structural examination and cell tracking experiments. In addition, an in vitro experiment with NRK‑52E cells was performed. The cells were divided into three groups, including the healthy control, cisplatin induction and cisplatin induction with co‑culture of AD‑MSCs, and were subsequently assessed with a Transwell assay. After culture for four days, the cells were lysed and the total protein extract was subjected to western blot analysis. Cisplatin‑induced renal dysfunction and tissue damage was shown to recover following AD‑MSC infusion, although there were few AD‑MSCs observed around the injured kidney tubules in the kidney. When the cisplatin‑treated NRK‑52E cells were co‑cultured with AD‑MSCs, the activation of p38 and BAX were inhibited, while the expression of Bcl‑2 was upregulated, as compared with the cisplatin‑treated NRK‑52E cells that were not co‑cultured. Therefore, AD‑MSCs were shown to markedly improve cisplatin‑induced renal failure and tubular cells necrosis through the secretion of certain factors, which subsequently inhibited the apoptosis pathway in vitro. It was hypothesized that AD‑MSC secretion was triggered by the injured tubular cells. Thus, AD‑MSCs may be important for the therapy of patients with renal injury due to their antiapoptotic capacity.
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1 

Lebwohl D and Canetta R: Clinical development of platinum complexes in cancer therapy: an historical perspective and an update. Eur J Cancer. 34:1522–1534. 1998. View Article : Google Scholar : PubMed/NCBI

2 

dos Santos NA, Carvalho Rodrigues MA, Martins NM and dos Santos AC: Cisplatin-induced nephrotoxicity and targets of nephroprotection: an update. Arch Toxicol. 86:1233–1250. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Heidemann HT, Müller S, Mertins L, Stepan G, Hoffmann K and Ohnhaus EE: Effect of aminophylline on cisplatin nephrotoxicity in the rat. Br J Pharmacol. 97:313–318. 1989. View Article : Google Scholar : PubMed/NCBI

4 

Francescato HD, Costa RS, Scavone C and Coimbra TM: Parthenolide reduces cisplatin-induced renal damage. Toxicology. 230:64–75. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Werner M, Costa MJ, Mitchell LG and Nayar R: Nephrotoxicity of xenobiotics. Clin Chim Acta. 237:107–154. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Jiang M and Dong Z: Regulation and pathological role of p53 in cisplatin nephrotoxicity. J Pharmacol Exp Ther. 327:300–307. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Clark JS, Faisal A, Baliga R, Nagamine Y and Arany I: Cisplatin induces apoptosis through the ERK-p66shc pathway in renal proximal tubule cells. Cancer Lett. 297:165–170. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Ramesh G and Reeves WB: TNF-alpha mediates chemokine and cytokine expression and renal injury in cisplatin nephrotoxicity. J Clin Invest. 110:835–842. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Zhang B, Ramesh G, Norbury CC and Reeves WB: Cisplatin-induced nephrotoxicity is mediated by tumor necrosis factor-alpha produced by renal parenchymal cells. Kidney Int. 72:37–44. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Zhuang S and Schnellmann RG: A death-promoting role for extracellular signal-regulated kinase. J Pharmacol Exp Ther. 319:991–997. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Morigi M, Imberti B, Zoja C, et al: Mesenchymal stem cells are renotropic, helping to repair the kidney and improve function in acute renal failure. J Am Soc Nephrol. 15:1794–1804. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Morigi M, Rota C, Montemurro T, et al: Life-sparing effect of human cord blood-mesenchymal stem cells in experimental acute kidney injury. Stem Cells. 28:513–522. 2010.PubMed/NCBI

13 

Peng X, Xu H, Zhou Y, et al: Human umbilical cord mesenchymal stem cells attenuate cisplatin-induced acute and chronic renal injury. Exp Biol Med (Maywood). 238:960–970. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Humphreys BD and Bonventre JV: Mesenchymal stem cells in acute kidney injury. Annu Rev Med. 59:311–325. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Kusaba T, Lalli M, Kramann R, Kobayashi A and Humphreys BD: Differentiated kidney epithelial cells repair injured proximal tubule. Proc Natl Acad Sci USA. 111:1527–1532. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Maeshima A, Yamashita S and Nojima Y: Identification of renal progenitor-like tubular cells that participate in the regeneration processes of the kidney. J Am Soc Nephrol. 14:3138–3146. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Tadagavadi RK and Reeves WB: Renal dendritic cells ameliorate nephrotoxic acute kidney injury. J Am Soc Nephrol. 21:53–63. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Zhu XY, Urbieta-Caceres V, Krier JD, Textor SC, Lerman A and Lerman LO: Mesenchymal stem cells and endothelial progenitor cells decrease renal injury in experimental swine renal artery stenosis through different mechanisms. Stem Cells. 31:117–125. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Altun B, Yilmaz R, Aki T, et al: Use of mesenchymal stem cells and darbepoetin improve ischemia-induced acute kidney injury outcomes. Am J Nephrol. 35:531–539. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Bian X, Zhang B, Guo W, et al: Effects of mesenchymal stem cells transplanted at different time points in a rat remnant kidney model. Am J Nephrol. 39:75–84. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Bussolati B, Tetta C and Camussi G: Contribution of stem cells to kidney repair. Am J Nephrol. 28:813–822. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Kitamura S, Yamasaki Y, Kinomura M, Sugaya T, Sugiyama H, Maeshima Y and Makino H: Establishment and characterization of renal progenitor like cells from S3 segment of nephron in rat adult kidney. FASEB J. 19:1789–1797. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Kim JH, Park DJ, Yun JC, et al: Human adipose tissue-derived mesenchymal stem cells protect kidneys from cisplatin nephrotoxicity in rats. Am J Physiol Renal Physiol. 302:F1141–F1150. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Reinders ME and Rabelink TJ: Adipose tissue-derived stem cells: can impure cell preparations give pure results? Nephrol Dial Transplant. 25:3805–3807. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Brzoska M, Geiger H, Gauer S and Baer P: Epithelial differentiation of human adipose tissue-derived adult stem cells. Biochem Biophys Res Commun. 330:142–150. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Huang HC, Chang YJ, Chen WC, Harn HI, Tang MJ and Wu CC: Enhancement of renal epithelial cell functions through microfluidic-based coculture with adipose-derived stem cells. Tissue Eng Part A. 19:2024–2034. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Katsuno T, Ozaki T, Saka Y, et al: Low serum cultured adipose tissue-derived stromal cells ameliorate acute kidney injury in rats. Cell Transplant. 22:287–297. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Shih YC, Lee PY, Cheng H, Tsai CH, Ma H and Tarng DC: Adipose-derived stem cells exhibit antioxidative and antiapoptotic properties to rescue ischemic acute kidney injury in rats. Plast Reconstr Surg. 132:940e–951e. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Shin S, Kim Y, Jeong S, Hong S, Kim I, Lee W and Choi S: The therapeutic effect of human adult stem cells derived from adipose tissue in endotoxemic rat model. Int J Med Sci. 10:8–18. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Wang W, Wang W, Jiang Y, Li Z, et al: Human adipose-derived stem cells modified by HIF-1α accelerate the recovery of cisplatin-induced acute renal injury in vitro. Biotechnol Lett. 36:667–676. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Zhang L, Li K, Liu X, et al: Repeated systemic administration of human adipose-derived stem cells attenuates overt diabetic nephropathy in rats. Stem Cells Dev. 22:3074–3086. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Zuk PA, Zhu M, Ashjian P, et al: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Morigi M, Introna M, Imberti B, et al: Human bone marrow mesenchymal stem cells accelerate recovery of acute renal injury and prolong survival in mice. Stem Cells. 26:2075–2082. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Qi S and Wu D: Bone marrow-derived mesenchymal stem cells protect against cisplatin-induced acute kidney injury in rats by inhibiting cell apoptosis. Int J Mol Med. 32:1262–1272. 2013.PubMed/NCBI

35 

Francescato HD, Costa RS, da Silva CG and Coimbra TM: Treatment with a p38 MAPK inhibitor attenuates cisplatin nephrotoxicity starting after the beginning of renal damage. Life Sci. 84:590–597. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Iwayama H and Ueda N: Role of mitochondrial Bax, caspases, and MAPKs for ceramide-induced apoptosis in renal proximal tubular cells. Mol Cell Biochem. 379:37–42. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao W, Hu Q, Ma Y, Xiong W, Wu T, Cao J and Wu D: Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways. Exp Ther Med 10: 468-476, 2015.
APA
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., & Wu, D. (2015). Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways. Experimental and Therapeutic Medicine, 10, 468-476. https://doi.org/10.3892/etm.2015.2505
MLA
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., Wu, D."Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways". Experimental and Therapeutic Medicine 10.2 (2015): 468-476.
Chicago
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., Wu, D."Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways". Experimental and Therapeutic Medicine 10, no. 2 (2015): 468-476. https://doi.org/10.3892/etm.2015.2505
Copy and paste a formatted citation
x
Spandidos Publications style
Yao W, Hu Q, Ma Y, Xiong W, Wu T, Cao J and Wu D: Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways. Exp Ther Med 10: 468-476, 2015.
APA
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., & Wu, D. (2015). Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways. Experimental and Therapeutic Medicine, 10, 468-476. https://doi.org/10.3892/etm.2015.2505
MLA
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., Wu, D."Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways". Experimental and Therapeutic Medicine 10.2 (2015): 468-476.
Chicago
Yao, W., Hu, Q., Ma, Y., Xiong, W., Wu, T., Cao, J., Wu, D."Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways". Experimental and Therapeutic Medicine 10, no. 2 (2015): 468-476. https://doi.org/10.3892/etm.2015.2505
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