Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Genomic and proteomic investigation of preeclampsia

  • Authors:
    • Hayrettin Sahin
    • Tuba Gunel
    • Ali Benian
    • Evren Onay Ucar
    • Onur Guralp
    • Aydinli Kilic
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul 34098, Turkey, Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul 34134, Turkey, Medicus Health Center, Istanbul 34365, Turkey
  • Pages: 711-716
    |
    Published online on: May 21, 2015
       https://doi.org/10.3892/etm.2015.2509
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to use proteomic and transcriptomic approaches to examine differences in protein and gene expression in maternal plasma between normal and preeclamptic pregnancies. Preeclampsia and control groups were compared with respect to the expression of CD34 and CD133 genes by quantitative polymerase chain reaction (qPCR) and heat shock protein (Hsp)27 and 70 by western blotting in blood samples from the pregnant women. Blood samples were obtained at gestational week 12‑14 from 65 healthy pregnant women. Fetal DNA was isolated from the maternal blood and CD34 and CD133 were amplified by qPCR. Western blot analysis was used to examine the expression levels of Hsp27 and Hsp70 proteins. The analysis of CD133 by qPCR was unsuccessful in 7 women as the levels of fetal DNA were in the collected maternal blood samples were insufficient. Measurements of CD34 and CD133 were performed in 57 and 50 women, respectively. Preeclampsia developed in 6 (10.5%) of 57 women. qPCR results of 8 healthy pregnant women were used for the calibration of CD34 and CD133 levels, and the results for the remaining women were compared with the calibration values. CD34 expression was decreased in 30 (52.6%) and increased in 27 (47.4%) of 57 women. CD133 expression was decreased in 14 (28%) and increased in 36 (72%) of 50 women. CD34 expression was increased in 2 (33%) and 25 (49%) and decreased in 4 (66%) and 25 (51%) women with and without preeclampsia, respectively (P=0.467). CD133 expression was increased in 4 (66%) and 32 (72%); and decreased in 2 (33%) and 12 (28%) women with and without preeclampsia, respectively (P=0.756). Western blotting showed that the expression of Hsp27 and Hsp70 in the maternal serum of the preeclampsia group was significantly higher than that in the normal pregnancy group. CD34 and CD133 were found to be inadequate for use in the prediction of preeclampsia. However, it is noteworthy that CD133 levels were increased in 66 and 72% of women with and without preeclampsia, respectively. Hsps are expressed under various pathological conditions. These results suggest that conditions of oxidative stress increased the Hsp27 and Hsp70 protein levels.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Roberts JM and Cooper DW: Pathogenesis and genetics of preeclampsia. Lancet. 357:53–56. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Salonen Ros H, Lichtenstein P, Lipworth L and Cnattingius S: Genetic effects on the liability of developing pre-eclampsia and gestational hypertension. Am J Med Genet. 4:256–260. 2000. View Article : Google Scholar

3 

Nguyen Huu S, Dubernard G, Aractingi S and Khosrotehrani K: Feto-maternal cell trafficking: A transfer of pregnancy associated progenitor cells. Stem Cell Rev. 2:111–116. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Gussin HA, Bischoff FZ, Hoffman R and Elias S: Endothelial precursor cells in the peripheral blood of pregnant women. J Soc Gynecol Invest. 9:357–361. 2002. View Article : Google Scholar

5 

Sugawara J, Mitsui-Saito M, Hoshiai T, Hayashi C, Kimura Y and Okamura K: Circulating endothelial progenitor cells during human pregnancy. J Clin Endocrinol Metab. 90:1845–1848. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Buemi M, Allegra A, D'Anna R, et al: Concentration of circulating endothelial progenitor cells (EPC) in normal pregnancy and in pregnant women with diabetes and hypertension. Am J Obstet Gynecol. 196:68.e1–68.e6. 2007. View Article : Google Scholar

7 

Zeybek YG, Günel T, Benian A, Aydınlı K and Kaleli S: Clinical evaluations of cell-free fetal quantities in pre-eclamptic pregnancies. J Obstet Gynaecol Res. 39:632–640. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Satterthwaite AB, Burn TC, Le Beau MM and Tenen DG: Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. Genomics. 12:788–794. 1992. View Article : Google Scholar : PubMed/NCBI

9 

Davey DA and MacGillivray I: The classification and definition of the hypertensive disorders of pregnancy. Am J Obstet Gynecol. 158:892–898. 1988. View Article : Google Scholar : PubMed/NCBI

10 

Bianchi DW, Zickwolf GK, Weil GJ, Sylvester S and DeMaria MA: Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum. Proc Natl Acad Sci USA. 93:705–708. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Miraglia S, Godfrey W, Yin AH, et al: A novel five-transmembrane hematopoietic stem cell antigen: Isolation, characterization, and molecular cloning. Blood. 90:5013–5021. 1997.PubMed/NCBI

12 

Yin AH, Miraglia S, Zanjani ED, et al: AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood. 90:5002–5012. 1997.PubMed/NCBI

13 

Ariga H, Ohto H, Busch MP, et al: Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy: Implications for noninvasive prenatal diagnosis. Transfusion. 41:1524–1530. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Luppi P, Powers RW, Verma V, Edmunds L, Plymire D and Hubel CA: Maternal circulating CD34+VEGFR-2+ and CD133+VEGFR-2+ progenitor cells increase during normal pregnancy but are reduced in women with preeclampsia. Reprod Sci. 17:643–652. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Wilhelmi MH, Mertsching H, Wilhelmi M, Leyh R and Haverich A: Role of inflammation in allogeneic and xenogeneic heart valve degeneration: Immunohistochemical evaluation of inflammatory endothelial cell activation. J Heart Valve Dis. 12:520–526. 2003.PubMed/NCBI

16 

Mazzone A, Epistolato MC, De Caterina R, et al: Neoangiogenesis, T-lymphocyte infiltration, and heat shock protein-60 are biological hallmarks of an immunomediated inflammatory process in end-stage calcified aortic valve stenosis. J Am Coll Cardiol. 43:1670–1676. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Papp E, Nardai G, Söti C and Csermely P: Molecular chaperones, stress proteins and redox homeostasis. Biofactors. 17:249–257. 2013. View Article : Google Scholar

18 

Molvarec A, Prohászka Z, Nagy B, Szalay J, Füst G, Karádi I and Rigó J Jr: Association of elevated serum heat-shock protein 70 concentration with transient hypertension of pregnancy, preeclampsia and superimposed preeclampsia: A case-control study. J Hum Hypertens. 20:780–786. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Fukushima A, Kawahara H, Isurugi C, et al: Changes in serum levels of heat shock protein 70 in preterm delivery and preeclampsia. J Obstet Gynaecol Res. 31:72–77. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Shin JK, Jeong YT, Jo HC, et al: Increased interaction between heat shock protein 27 and mitogen-activated protein kinase (p38 and extracellular signal-regulated kinase) in pre-eclamptic placentas. J Obstet Gynaecol Res. 35:888–894. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Cunningham FG, Leveno KJ, Bloom SL, Hauth JC, Gilstrap LC III and Wenstrom KD: Hypertensive disorders in pregnancyWilliams Obstetrics. 22nd. McGraw Hill; New York: pp. 761–808. 2005

22 

Lindheimer MD, Roberts JM and Cunningham FG: Prevention of preeclampsia and eclampsiaChesley's Hypertensive Disorders in Pregnancy. 3rd. Elsevier (Academic Press); San Diego, CA: pp. 213–226. 2009

23 

Majka M, Janowska-Wieczorek A, Ratajczak J, et al: Numerous growth factors, cytokines, and chemokines are secreted by human CD34+ cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner. Blood. 97:3075–3085. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Peichev M, Naiyer AJ, Pereira D, et al: Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors. Blood. 95:952–958. 2000.PubMed/NCBI

25 

Jászai J, Fargeas CA, Florek M, Huttner WB and Corbeil D: Focus on molecules: Prominin-1 (CD133). Exp Eye Res. 85:585–586. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Lo YM, Zhang J, Leung TN, Lau TK, Chang AM and Hjelm NM: Rapid clearance of fetal DNA from maternal plasma. Am J Hum Genet. 64:218–224. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Hahn S, Gupta AK, Troeger C, et al: Disturbances in placental immunology: Ready for therapeutic interventions? Springer Semin Immunopathol. 27:477–493. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Carty DM, Delles C and Dominiczak AF: Novel biomarkers for predicting preeclampsia. Trends Cardiovasc Med. 18:186–194. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Zhong XY, Gebhardt S, Hillermann R, Tofa KC, Holzgreve W and Hahn S: Parallel assessment of circulatory fetal DNA and corticotropin-releasing hormone mRNA in early- and late-onset preeclampsia. Clin Chem. 51:1730–1733. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Sifakis S, Zaravinos A, Maiz N, Spandidos DA and Nicolaides KH: First-trimester maternal plasma cell-free fetal DNA and preeclampsia. Am J Obstet Gynecol. 201:472.e1–472.e7. 2009. View Article : Google Scholar

31 

Horinek A, Korabecna M, Panczak A, et al: Cell-free fetal DNA in maternal plasma during physiological single male pregnancies: Methodology issues and kinetics. Fetal Diagn Ther. 24:15–21. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Pomyje J, Zivný J, Sefc L, Plasilová M, Pytlík R and Necas E: Expression of genes regulating angiogenesis in human circulating hematopoietic cord blood CD34+/CD133+ cells. Eur J Haematol. 70:143–150. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Matsubara K, Abe E, Matsubara Y, Kameda K and Ito M: Circulating endothelial progenitor cells during normal pregnancy and pre-eclampsia. Am J Reprod Immunol. 56:79–85. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Bauriedel G, Jabs A, Skowasch D, et al: Dendritic cells in neointima formation after rat carotid balloon injury. Coordinated expression with anti-apoptotic Bcl-2 and HSP47 in arterial repair. J Am Coll Cardiol. 42:930–938. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Banchereau J, Briere F, Caux C, et al: Immunobiology of dendritic cells. Annu Rev Immunol. 18:767–811. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Ekambaram P: HSP70 expression and its role in preeclamptic stress. Indian J Biochem Biophys. 48:243–255. 2011.PubMed/NCBI

37 

Shin JK, Jeong YT, Jo HC, et al: Increased interaction between heat shock protein 27 and mitogen-activated protein kinase (p38 and extracellular signal-regulated kinase) in pre-eclamptic placentas. J Obstet Gynaecol Res. 35:888–894. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sahin H, Gunel T, Benian A, Onay Ucar E , Guralp O and Kilic A: Genomic and proteomic investigation of preeclampsia. Exp Ther Med 10: 711-716, 2015.
APA
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., & Kilic, A. (2015). Genomic and proteomic investigation of preeclampsia. Experimental and Therapeutic Medicine, 10, 711-716. https://doi.org/10.3892/etm.2015.2509
MLA
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., Kilic, A."Genomic and proteomic investigation of preeclampsia". Experimental and Therapeutic Medicine 10.2 (2015): 711-716.
Chicago
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., Kilic, A."Genomic and proteomic investigation of preeclampsia". Experimental and Therapeutic Medicine 10, no. 2 (2015): 711-716. https://doi.org/10.3892/etm.2015.2509
Copy and paste a formatted citation
x
Spandidos Publications style
Sahin H, Gunel T, Benian A, Onay Ucar E , Guralp O and Kilic A: Genomic and proteomic investigation of preeclampsia. Exp Ther Med 10: 711-716, 2015.
APA
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., & Kilic, A. (2015). Genomic and proteomic investigation of preeclampsia. Experimental and Therapeutic Medicine, 10, 711-716. https://doi.org/10.3892/etm.2015.2509
MLA
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., Kilic, A."Genomic and proteomic investigation of preeclampsia". Experimental and Therapeutic Medicine 10.2 (2015): 711-716.
Chicago
Sahin, H., Gunel, T., Benian, A., Onay Ucar, E. ., Guralp, O., Kilic, A."Genomic and proteomic investigation of preeclampsia". Experimental and Therapeutic Medicine 10, no. 2 (2015): 711-716. https://doi.org/10.3892/etm.2015.2509
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team