Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats

  • Authors:
    • Yong‑Guang Zhang
    • Qing‑Song Sheng
    • Zhi‑Jun Wang
    • Li Lv
    • Wei Zhao
    • Jian‑Mei Chen
    • Hao Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Fuzhou General Hospital of Nanjing Military Command/Dongfang Hospital of Xiamen University, Fuzhou, Fujian 350025, P.R. China, Department of Obstetrics and Gynecology, Fuzhou General Hospital of Nanjing Military Command/Dongfang Hospital of Xiamen University, Fuzhou, Fujian 350025, P.R. China, Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Key Laboratory of Reproduction and Heredity of Ningxia Hui Autonomous Region, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China
  • Pages: 727-732
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/etm.2015.2518
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Despite the application of nerve grafts and considerable microsurgical innovations, the functional recovery across a long peripheral nerve gap is generally partial and unsatisfactory. Thus, additional strategies are required to improve nerve regeneration across long nerve gaps. Hydrogen possesses antioxidant and anti‑apoptotic properties, which could be neuroprotective in the treatment of peripheral nerve injury; however, such a possibility has not been experimentally tested in vivo. The aim of the present study was to investigate the effectiveness of hydrogen‑rich saline in promoting nerve regeneration after 10‑mm sciatic nerve autografting in rats. The rats were randomly divided into two groups and intraperitoneally administered a daily regimen of 5 ml/kg hydrogen‑rich or normal saline. Axonal regeneration and functional recovery were assessed through a combination of behavioral analyses, electrophysiological evaluations, Fluoro‑Gold™ retrograde tracings and histomorphological observations. The data showed that rats receiving hydrogen‑rich saline achieved better axonal regeneration and functional recovery than those receiving normal saline. These findings indicated that hydrogen‑rich saline promotes nerve regeneration across long gaps, suggesting that hydrogen‑rich saline could be used as a neuroprotective agent for peripheral nerve injury therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Alluin O, Wittmann C, Marqueste T, et al: Functional recovery after peripheral nerve injury and implantation of a collagen guide. Biomaterials. 30:363–373. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Kehoe S, Zhang XF and Boyd D: FDA approved guidance conduits and wraps for peripheral nerve injury: A review of materials and efficacy. Injury. 43:553–572. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Tang X, Xue C, Wang Y, Ding F, Yang Y and Gu X: Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold. Biomaterials. 33:3860–3867. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Cui Q, Zhang J, Zhang L, Li R and Liu H: Angelica injection improves functional recovery and motoneuron maintenance with increased expression of brain derived neurotrophic factor and nerve growth factor. Curr Neurovasc Res. 6:117–123. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Nie X, Zhang YJ, Tian WD, et al: Improvement of peripheral nerve regeneration by a tissue-engineered nerve filled with ectomesenchymal stem cells. Int J Oral Maxillofac Surg. 36:32–38. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Hart AM, Terenghi G and Wiberg M: Neuronal death after peripheral nerve injury and experimental strategies for neuroprotection. Neurol Res. 30:999–1011. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Ohsawa I, Ishikawa M, Takahashi K, et al: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 13:688–694. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Fukuda K, Asoh S, Ishikawa M, Yamamoto Y, Ohsawa I and Ohta S: Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress. Biochem Biophys Res Commun. 361:670–674. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Sato Y, Kajiyama S, Amano A, et al: Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice. Biochem Biophys Res Commun. 375:346–350. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Cai J, Kang Z, Liu WW, et al: Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model. Neurosci Lett. 441:167–172. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Chen C, Chen Q, Mao Y, et al: Hydrogen-rich saline protects against spinal cord injury in rats. Neurochem Res. 35:1111–1118. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Yonamine R, Satoh Y, Kodama M, Araki Y and Kazama T: Coadministration of hydrogen gas as part of the carrier gas mixture suppresses neuronal apoptosis and subsequent behavioral deficits caused by neonatal exposure to sevoflurane in mice. Anesthesiology. 118:105–113. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Sun Q, Kang Z, Cai J, et al: Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats. Exp Biol Med (Maywood). 234:1212–1219. 2009. View Article : Google Scholar : PubMed/NCBI

14 

de Medinaceli L, Freed WJ and Wyatt RJ: An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol. 77:634–643. 1982. View Article : Google Scholar : PubMed/NCBI

15 

Hare GM, Evans PJ, Mackinnon SE, et al: Walking track analysis: A long-term assessment of peripheral nerve recovery. Plast Reconstr Surg. 89:251–258. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Suzuki Y, Tanihara M, Ohnishi K, Suzuki K, Endo K and Nishimura Y: Cat peripheral nerve regeneration across 50 mm gap repaired with a novel nerve guide composed of freeze-dried alginate gel. Neurosci Lett. 259:75–78. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Huang J, Lu L, Hu X, et al: Electrical stimulation accelerates motor functional recovery in the rat model of 15-mm sciatic nerve gap bridged by scaffolds with longitudinally oriented microchannels. Neurorehabil Neural Repair. 24:736–745. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Novikova L, Novikov L and Kellerth JO: Persistent neuronal labeling by retrograde fluorescent tracers: A comparison between Fast Blue, Fluoro-Gold and various dextran conjugates. J Neurosci Methods. 74:9–15. 1997. View Article : Google Scholar : PubMed/NCBI

19 

Fu SY and Gordon T: The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol. 14:67–116. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Nicholson DW and Thornberry NA: Caspases: Killer proteases. Trends Biochem Sci. 22:299–306. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Vukosavic S, Dubois-Dauphin M, Romero N and Przedborski S: Bax and Bcl-2 interaction in a transgenic mouse model of familial amyotrophic lateral sclerosis. J Neurochem. 73:2460–2468. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Naik AK, Tandan SK, Dudhgaonkar SP, et al: Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats. Eur J Pain. 10:573–579. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Senoglu M, Nacitarhan V, Kurutas EB, Senoglu N, Altun I, Atli Y and Ozbag D: Intraperitoneal alpha-lipoic acid to prevent neural damage after crush injury to the rat sciatic nerve. J Brachial Plex Peripher Nerve Inj. 4:222009. View Article : Google Scholar : PubMed/NCBI

24 

Formichi P, Battisti C, Radi E, Di Maio G and Federico A: Apoptosis, oxidative stress and neurological disease. J Siena Acad Sci. 1:40–45. 2009. View Article : Google Scholar

25 

Bromberg MB: Quantitative ElectromyographyElectrodiagnosis in Clinical Neurology. Aminoff MJ: 4th. Churchill Livingstone; New York, NY: pp. 257–263. 1999

26 

Rosen JM, Pham HN and Hentz VR: Fascicular tubulization: A comparison of experimental nerve repair techniques in the cat. Ann Plast Surg. 22:467–478. 1989. View Article : Google Scholar : PubMed/NCBI

27 

Matsumoto K, Ohnishi K, Kiyotani T, et al: Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: A histological and electrophysiological evaluation of regenerated nerves. Brain Res. 868:315–328. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Sherman DL and Brophy PJ: Mechanisms of axon ensheathment and myelin growth. Nat Rev Neurosci. 6:683–690. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang YG, Sheng QS, Wang ZJ, Lv L, Zhao W, Chen JM and Xu H: Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats. Exp Ther Med 10: 727-732, 2015.
APA
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., & Xu, H. (2015). Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats. Experimental and Therapeutic Medicine, 10, 727-732. https://doi.org/10.3892/etm.2015.2518
MLA
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., Xu, H."Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats". Experimental and Therapeutic Medicine 10.2 (2015): 727-732.
Chicago
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., Xu, H."Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats". Experimental and Therapeutic Medicine 10, no. 2 (2015): 727-732. https://doi.org/10.3892/etm.2015.2518
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang YG, Sheng QS, Wang ZJ, Lv L, Zhao W, Chen JM and Xu H: Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats. Exp Ther Med 10: 727-732, 2015.
APA
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., & Xu, H. (2015). Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats. Experimental and Therapeutic Medicine, 10, 727-732. https://doi.org/10.3892/etm.2015.2518
MLA
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., Xu, H."Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats". Experimental and Therapeutic Medicine 10.2 (2015): 727-732.
Chicago
Zhang, Y., Sheng, Q., Wang, Z., Lv, L., Zhao, W., Chen, J., Xu, H."Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats". Experimental and Therapeutic Medicine 10, no. 2 (2015): 727-732. https://doi.org/10.3892/etm.2015.2518
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team