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Article

Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head

  • Authors:
    • Rui Bai
    • Wanlin Liu
    • Aiqing Zhao
    • Zhengqun Zhao
    • Dianming Jiang
  • View Affiliations / Copyright

    Affiliations: Graduate School of Chongqing Medical University, Chongqing, P.R. China, Department of Pediatric Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, P.R. China, Department of Computed Tomography, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, P.R. China, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China
  • Pages: 591-597
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/etm.2015.2521
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Abstract

The aim of the present study was to explore the effect on nitric oxide (NO) content and osteocyte apoptosis of steroid‑induced avascular necrosis of the femoral head (SANFH) in an animal model of SANFH. A total of 40 Japanese white rabbits, 5 months of age and weighing 2.5±0.5 kg, were randomly divided into groups A (hormone + endotoxin group), B (endotoxin + normal saline group), C (normal saline + hormone group) and D (control group). Following the establishment of the model, a blood sample was taken from the heart of each animal and centrifuged; the levels of NO in the serum were detected. The bilateral femoral heads were conventionally dissected, fixed, decalcified and stained with hematoxylin and eosin. Subsequently, the empty bone lacunae were counted under an optical microscope. Changes in osteocyte morphology were observed using electron microscopy and osteocyte apoptosis was detected with a terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The percentage of empty bone lacunae in group A was significantly higher compared with that in groups B, C and D (P<0.01); however, there was no significant difference in percentage among groups B, C and D. The NO content in group A was significantly higher compared with that in groups B, C and D (P<0.01); however, there was no significant difference in NO content among groups B, C and D. The osteocyte apoptosis index in group A was significantly higher compared with that in the other groups (P<0.01); there was no significant difference among groups B, C and D. NO content was positively correlated with osteocyte apoptosis index (r=0.707). Thus, the present study found that NO content and the osteocyte apoptosis index were increased in SANFH, and that they play an important role in SANFH. The content of NO was positively correlated with the osteocyte apoptosis index, indicating that NO induces apoptosis.
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1 

Chen S, Li J, Peng H, Zhou J and Fang H: Administration of erythropoietin exerts protective effects against glucocorticoid-induced osteonecrosis of the femoral head in rats. Int J Mol Med. 33:840–848. 2014.PubMed/NCBI

2 

Saito M, Ueshima K, Fujioka M, et al: Corticosteroid administration within 2 weeks after renal transplantation affects the incidence of femoral head osteonecrosis. Acta Orthop. 85:266–270. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Nishida K, Yamamoto T, Motomura G, Jingushi S and Iwamoto Y: Pitavastatin may reduce risk of steroid-induced osteonecrosis in rabbits: A preliminary histological study. Clin Orthop Relat Res. 466:1054–1058. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Takano-Murakami R, Tokunaga K, Kondo N, Ito T, et al: Glucocoriticoid inhibits bone regeneration after osteonecrosis of the femoral head in aged female rats. Tohoku J Exp Med. 217:51–58. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Chan KL and Mok CC: Glucocorticoid-induced avascular bone necrosis: Diagnosis and management. Open Orthop J. 6:449–457. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Chen C, Yang S, Feng Y, et al: Impairment of two types of circulating endothelial progenitor cells in patients with glucocorticoid-induced avascular osteonecrosis of the femoral head. Joint Bone Spine. 80:70–76. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Tan G, Kang PD and Pei FX: Glucocorticoids affect the metabolism of bone marrow stromal cells and lead to osteonecrosis of the femoral head: A review. Chin Med J (Engl). 125:134–139. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Zou W, Yang S, Zhang T, et al: Hypoxia enhances glucocorticoid-induced apoptosis and cell cycle arrest via the PI3K/Akt signaling pathway in osteoblastic cells. J Bone Miner Metab Sep. 18:2014.(Epub ahead of print).

9 

Ye J, Wu G, Li X, et al: Millimeter wave treatment inhibits apoptosis of chondrocytes via regulation dynamic equilibrium of intracellular free Ca2+. Evid Based Complement Alternat Med. 2015:4641612015. View Article : Google Scholar : PubMed/NCBI

10 

Ohzono K, Takaoka K, Saito S, Saito M, Matsui M and Ono K: Intraosseous arterial architecture in nontraumatic avascular necrosis of the femoral head. Microangiographic and histologic study. Clin Orthop Relat Res. 277:79–88. 1992.PubMed/NCBI

11 

Eberhardt AW, Yeager-Jones A and Blair HC: Regional trabecular bone matrix degeneration and osteocyte death in femora of glucocorticoid-treated rabbits. Endocrinology. 142:1333–1340. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Pietrograndi V and Mastromarino R: Osteopatia da prolungata trattamento cortisonico. Ortop Traum Appar Mar. 25:791–810. 1957.(In Italian).

13 

Fukushima W, Fujioka M, Kubo T, et al: Nationwide epidemiologic survey of idiopathic osteonecrosis of the femoral head. Clin Orthop Relat Res. 468:2715–2724. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Qiu NH and Zhang W: Infectious atypical pneumonia after the etiology and treatment of femoral head necrosis. Chinese J Tissue Eng Res. 17:5525–5530. 2013.

15 

Weinstein RS: Glucocorticoid-induced osteonecrosis. Endocrine. 41:183–190. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Kerachian MA, Cournoyer D, Harvey EJ, et al: New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: Microarray analysis of gene expression in a rat model. Arthritis Res Ther. 12:R1242010. View Article : Google Scholar : PubMed/NCBI

17 

Kameda H, Amano K, Nagasawa H, et al: Notable difference between the development of vertebral fracture and osteonecrosis of the femoral head in patients treated with high-dose glucocorticoids for systemic rheumatic diseases. Intern Med. 48:1931–1938. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Chen RM, Liu HC, Lin YL, Jean WC, Chen JS and Wang JH: Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein. J Orthop Res. 20:295–302. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Buerk DG, Barbee KA and Jaron D: Nitric oxide signaling in the microcirculation. Crit Rev Biomed Eng. 39:397–433. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Bao F, Wu P, Xiao N, Qiu F and Zeng QP: Nitric oxide-driven hypoxia initiates synovial angiogenesis, hyperplasia and inflammatory lesions in mice. PLoS One. 7:e344942012. View Article : Google Scholar : PubMed/NCBI

21 

Calder JD, Buttery L, Revell PA, et al: Apoptosis - a significant cause of bone cell death in osteonecrosis of femoral head. J Bone Joint Surg Br. 86:1209–1213. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Hu M, Zhao H, Dong X, Luo D and Zhou X: General and light microscope observation on histological changes of femoral heads between SANFH rabbit animal models and it were intervened by Osteoking Zhongguo Zhong Yao Za Zhi. 35:2912–2916. 2010.(In Chinese). PubMed/NCBI

23 

Lee JS, Lee JS, Roh HL, Kim CH, Jung JS and Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients with nontraumatic osteonecrosis of the femoral head: Comparative analysis according to the risk factor. J Orthop Res. 24:604–609. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bai R, Liu W, Zhao A, Zhao Z and Jiang D: Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head. Exp Ther Med 10: 591-597, 2015.
APA
Bai, R., Liu, W., Zhao, A., Zhao, Z., & Jiang, D. (2015). Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head. Experimental and Therapeutic Medicine, 10, 591-597. https://doi.org/10.3892/etm.2015.2521
MLA
Bai, R., Liu, W., Zhao, A., Zhao, Z., Jiang, D."Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head". Experimental and Therapeutic Medicine 10.2 (2015): 591-597.
Chicago
Bai, R., Liu, W., Zhao, A., Zhao, Z., Jiang, D."Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head". Experimental and Therapeutic Medicine 10, no. 2 (2015): 591-597. https://doi.org/10.3892/etm.2015.2521
Copy and paste a formatted citation
x
Spandidos Publications style
Bai R, Liu W, Zhao A, Zhao Z and Jiang D: Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head. Exp Ther Med 10: 591-597, 2015.
APA
Bai, R., Liu, W., Zhao, A., Zhao, Z., & Jiang, D. (2015). Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head. Experimental and Therapeutic Medicine, 10, 591-597. https://doi.org/10.3892/etm.2015.2521
MLA
Bai, R., Liu, W., Zhao, A., Zhao, Z., Jiang, D."Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head". Experimental and Therapeutic Medicine 10.2 (2015): 591-597.
Chicago
Bai, R., Liu, W., Zhao, A., Zhao, Z., Jiang, D."Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head". Experimental and Therapeutic Medicine 10, no. 2 (2015): 591-597. https://doi.org/10.3892/etm.2015.2521
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