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siRNA targeting of Trop2 suppresses the proliferation and invasion of lung adenocarcinoma H460 cells

  • Authors:
    • Xiao‑Yan Gao
    • Ye‑Han Zhu
    • Li‑Xin Zhang
    • Hui‑Yu Lu
    • Ai‑Gui Jiang

  • View Affiliations

  • Published online on: May 28, 2015     https://doi.org/10.3892/etm.2015.2530
  • Pages: 429-434

  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of the small interfering RNA (siRNA)‑induced inhibition of the Trop2 gene on the proliferation and invasion of lung adenocarcinoma H460 cells. A recombinant adenovirus expression vector, which contained siRNA targeting open reading frames for Trop2 (rAd5‑siTrop2), was transfected into lung adenocarcinoma H460 cells. Three groups were included in the study, namely the Ctrl (non‑transfected control), rAd5‑siCtrl (native control) and rAd5‑siTrop2 (knockdown Trop2 gene) groups. The mRNA and protein expression levels of Trop2 were detected using quantitative polymerase chain reaction and western blot analysis, respectively. In addition, the expression levels of cyclin Dl and phospho‑extracellular signal regulated kinase (p‑ERK)‑1 were detected using western blot analysis. The effects of Trop2 inhibition on the proliferation and invasion of lung adenocarcinoma H460 cells were investigated using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and Transwell assay. Trop2‑targeted siRNA recombinant plasmids were successfully constructed. The recombinant adenovirus vector, rAd5‑siTrop2, significantly downregulated the mRNA and protein expression levels of Trop2 in the lung adenocarcinoma H460 cells, with cyclin D1 and p‑ERK‑1 expression downregulated simultaneously. In addition, following the silencing of Trop2, the proliferation and invasion rates of the lung adenocarcinoma H460 cells were reduced. Therefore, the results indicated that Trop2 serves a key function in the proliferation and invasion of lung adenocarcinoma H460 cells in vitro.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao XY, Zhu YH, Zhang LX, Lu HY and Jiang AG: siRNA targeting of Trop2 suppresses the proliferation and invasion of lung adenocarcinoma H460 cells. Exp Ther Med 10: 429-434, 2015
APA
Gao, X., Zhu, Y., Zhang, L., Lu, H., & Jiang, A. (2015). siRNA targeting of Trop2 suppresses the proliferation and invasion of lung adenocarcinoma H460 cells. Experimental and Therapeutic Medicine, 10, 429-434. https://doi.org/10.3892/etm.2015.2530
MLA
Gao, X., Zhu, Y., Zhang, L., Lu, H., Jiang, A."siRNA targeting of Trop2 suppresses the proliferation and invasion of lung adenocarcinoma H460 cells". Experimental and Therapeutic Medicine 10.2 (2015): 429-434.
Chicago
Gao, X., Zhu, Y., Zhang, L., Lu, H., Jiang, A."siRNA targeting of Trop2 suppresses the proliferation and invasion of lung adenocarcinoma H460 cells". Experimental and Therapeutic Medicine 10, no. 2 (2015): 429-434. https://doi.org/10.3892/etm.2015.2530