Effect of hyperbaric oxygen preconditioning on peri-hemorrhagic focal edema and aquaporin-4 expression

  • Authors:
    • Jinyong Fang
    • Hongling Li
    • Guanglei Li
    • Lichun Wang
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  • Published online on: June 3, 2015     https://doi.org/10.3892/etm.2015.2539
  • Pages: 699-704
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Abstract

The aim of the present study was to investigate the effect of hyperbaric oxygen preconditioning (HBO‑PC) on peri‑hemorrhagic focal edema and aquaporin‑4 (AQP‑4) expression in an experimental intracerebral hemorrhage (ICH) rat model. Sixty‑six Sprague Dawley® rats were divided into three groups: The sham‑surgery group (SHG; n=6); the control group (A‑ICH; n=30), in which the rats were injected with autologous blood; and the experimental HBO‑PC group (P‑HBO; n=30). The rats underwent brain edema and AQP‑4 detection at 5 postoperative time‑points (24, 48 and 72 h and 5 and 7 days). The water content in the brain tissues of the A‑ICH animals was higher than that in the brain tissues of the SHG rats at each time‑point (P<0.05), and the edema in the P‑HBO was significantly more severe 24 and 48 h postoperatively than that at 7 days postoperatively (P<0.05). The difference between the P‑HBO and A‑ICH was significant at 48 and 72 h postoperatively (P<0.05). AQP‑4 was expressed in the post‑hemorrhagic rat brains of all groups; the SHG animals exhibited low expression, while the A‑ICH animals exhibited an increased expression 24 h postoperatively. In the A‑ICH, expression peaked at 48 h postoperatively and began to decrease gradually after 72 h. At the 7‑day time‑point, the expression level in the A‑ICH was closer to but still higher than that of the SHG animals (P<0.05). The differences between the P‑HBO and A‑ICH animals at the postoperative 24‑h, 48‑h and 7‑day time‑points were statistically significant (P<0.05). In conclusion, HBO‑PC may downregulate AQP‑4 expression to reduce the intracerebral edema, thus strengthening tolerance to ICH and protecting the nerves.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Fang J, Li H, Li G and Wang L: Effect of hyperbaric oxygen preconditioning on peri-hemorrhagic focal edema and aquaporin-4 expression. Exp Ther Med 10: 699-704, 2015
APA
Fang, J., Li, H., Li, G., & Wang, L. (2015). Effect of hyperbaric oxygen preconditioning on peri-hemorrhagic focal edema and aquaporin-4 expression. Experimental and Therapeutic Medicine, 10, 699-704. https://doi.org/10.3892/etm.2015.2539
MLA
Fang, J., Li, H., Li, G., Wang, L."Effect of hyperbaric oxygen preconditioning on peri-hemorrhagic focal edema and aquaporin-4 expression". Experimental and Therapeutic Medicine 10.2 (2015): 699-704.
Chicago
Fang, J., Li, H., Li, G., Wang, L."Effect of hyperbaric oxygen preconditioning on peri-hemorrhagic focal edema and aquaporin-4 expression". Experimental and Therapeutic Medicine 10, no. 2 (2015): 699-704. https://doi.org/10.3892/etm.2015.2539