Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis

  • Authors:
    • Wei Ding
    • Xing‑Yi Zhang
    • Ming Pan
    • Bin Zhao
    • Chuang Chen
    • Zhi‑Hao Niu
    • Cheng‑Liang Huang
    • Yan‑Yan Li
    • Xian‑Ming Fan
    • Yan‑Mei Ma
    • Ming Zhang
    • Wen‑Jun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Second Hospital, Jilin University, Changchun, Jilin 130022, P.R. China, Department of Respiratory Medicine, The Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 491-497
    |
    Published online on: June 10, 2015
       https://doi.org/10.3892/etm.2015.2564
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the effects of interleukin (IL)‑17A in a rat model of pulmonary fibrosis. In total, 20 female Wistar rats were randomly divided into a normal saline (NS group) and a bleomycin group (BLM group). The BLM group rats were intratracheally instilled with BLM, while the NS group rats were intratracheally instilled with saline. In each group, half the rats were sacrificed at day 7 and day 28, respectively, following intratracheal instillation. Subsequently, hematoxylin and eosin and Masson's trichrome staining were performed to observe the pathological changes in the lung tissue, while the expression of IL‑17A in the lung tissue was detected by immunohistochemistry. In addition, the bronchoalveolar lavage fluid (BALF) was collected and divided into two sections. One section was used for cell counting and classification, and an ELISA was performed to detect the concentration of IL‑17A in the BALF. The additional section was used to separate, purify and cultivate alveolar macrophages (AMs). The concentration of IL‑17A in the cultivating supernatant was detected by ELISA, and the mRNA expression levels of IL‑17A in the AMs were detected using reverse transcription‑polymerase chain reaction (RT‑PCR). The results revealed that a considerable number of inflammatory cells had infiltrated into the alveolar cavity in the BLM group at day 7, and less alveolitis and more serious fibrosis were observed at day 28, as compared with the NS group. Furthermore, when compared with the NS group, the protein expression levels of IL‑17A in the lung tissue were markedly higher in the BLM group at days 7 and 28 (higher at day 7; P<0.05). In addition, the total number of BALF cells in the BLM group was clearly higher at day 7 when compared with the NS group (P<0.05), although a normal level was re‑established by day 28. The level of IL‑17A in the BALF increased significantly at days 7 and 28 in the BLM group; however, when compared with the level at day 7, the concentration had decreased at day 28. When compared with the NS group, the protein expression levels of IL‑17A in the BLM group were notably higher after 12, 24 and 48 h. In addition, the results of the RT‑PCR assay revealed that the mRNA expression levels of IL‑17A increased significantly at days 7 and 28 in the BLM group when compared with the NS group (P<0.05). Therefore, IL‑17A was demonstrated to promote the development of pulmonary inflammation, which may be involved in the development of pulmonary fibrosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Tanaka K, Ishihara T, Azuma A, Kudoh S, Ebina M, Nukiwa T, Sugiyama Y, Tasaka Y, Namba T, Ishihara T, Sato K, Mizushima Y and Mizushima T: Therapeutic effect of lecithinized superoxide dismutase on bleomycin-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Hysiol. 298:L348–L360. 2010. View Article : Google Scholar

2 

Raghu G, Collard HR, Egan JJ, Martinez FJ, Behr J, et al: ATS/ERS/JRS/ALAT Committee on Idiopathic Pulmonary Fibrosis: An official ATS/ERS/JRS/ALAT statement: Idiopathic pulmonary fibrosis: Evidence-based guidelines for diagnosis and management. Am J Respir Crit Care Med. 183:788–824. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Mi S, Li Z, Yang HZ, Liu H, Wang JP, Ma YG, Wang XX, Liu HZ, Sun W and Hu ZW: Blocking IL-17A promotes the resolution of pulmonary inflammation and fibrosis via TGF-beta 1-dependent and -independent mechanisms. J Immunol. 187:3003–3014. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Umezawa H, Maeda K, Takeuchi T and Okami Y: New antibiotics, bleomycin A and B. J Antibiot (Tokyo). 19:200–209. 1966.PubMed/NCBI

5 

Hoshino T, Okamoto M, Sakazaki Y, Kato S, Young HA and Aizawa H: Role of proinflammatory cytokines IL-18 and IL-1beta in bleomycin-induced lung injury in humans and mice. Am J Respir Cell Mol Biol. 41:661–670. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Huang CL, Wang WJ, Zhu HL, Fan XM, Chen JP, Zhan XQ and Li YY: Effect of andrographolide on expression of hydroxyproline and PDGF in lung tissue of bleomycin-induced rat pulmonary fibrosis. Shizhen Guo Yi Guo Yao. 23:904–907. 2012.(In Chinese).

7 

Huang C, Li Y, Fan X, Wang W and Zhan X: Effects of combination of Salvia and Ligustrazine on TNF-α and TGF-β1 in serum and BALF of rats with pulmonary fibrosis. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 29:673–676. 2013.(In Chinese). PubMed/NCBI

8 

Tabata C, Kubo H, Tabata R, Wada M, Sakuma K, Ichikawa M, Fujita S, Mio T and Mishima M: All-trans retinoic acid modulates radiation-induced proliferation of lung fibroblasts via IL-6/IL-6R system. Am J Physiol Lung Cell Mol Physiol. 290:L597–L606. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Lee KY, Ito K, Hayashi R, Jazrawi EP, Barnes PJ and Adcock M: NF-kappa B and activator protein 1 response elements and the role of histone modifications in IL-1 beta-induced TGF-beta 1 gene transcription. J Immunol. 176:603–615. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Liu JP, Di NL, Fan XM, Li SC and Zhang SP: The expressions of STAT1 and PDGF in lung tissue of rats with pulmonary fibrosis induced by bleomycin. Shan Dong Yi Yao. 31:16–18. 2008.(In Chinese).

11 

Halwani R, Al-Muhsen S and Hamid Q: T helper 17 cells in airway diseases: From laboratory bench to bedside. Chest. 143:494–501. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Truchetet ME, Mossalayi MD and Boniface K: IL-17 in the rheumatologist's line of sight. Biomed Res Int. 2013:2951322013. View Article : Google Scholar : PubMed/NCBI

13 

Baeten D, Baraliakos X, Braun J, Sieper J, Emery P, et al: Anti-interleukin-17A monoclonal antibody secukinumab in treatment of ankylosing spondylitis: A randomised, double-blind, placebo-controlled trial. Lancet. 382:1705–1713. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Leonardi C, Matheson R, Zachariae C, Cameron G, Li L, Edson-Heredia E, Braun D and Banerjee S: Anti-interleukin-17 monoclonal antibody ixekizumab in chronic plaque psoriasis. N Engl J Med. 366:1190–1199. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Papp KA, Leonardi C, Menter A, Ortonne JP, Krueger JG, Kricorian G, Aras G, Li J, Russell CB, Thompson EH and Baumgartner S: Brodalumab, an anti-interleukin-17-receptor antibody for psoriasis. N Engl J Med. 366:1181–1189. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Wilson MS, Madala SK, Ramalingam TR, Gochuico BR, Rosas IO, Cheever AW and Wynn TA: Bleomycin and IL-1β-mediated pulmonary fibrosis is IL-17A dependent. J Exp Med. 207:535–552. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Gasse P, Riteau N, Vacher R, Michel ML, Fautrel A, di Padova F, Fick L, Charron S, Lagente V, Eberl G, Le Bert M, Quesniaux VF, Huaux F, Leite-de-Moraes M, Ryffel B and Couillin I: IL-1 and IL-23 mediate early IL-17A production in pulmonary inflammation leading to late fibrosis. PLoS One. 6:e231852011. View Article : Google Scholar : PubMed/NCBI

18 

Liu H, Li Z, Lv XX and Mi S: Neutralization of IL-17A activity can improve pulmonary function in mice with pulmonary fibrosis. Hang Tian Yi Xue Yu Yi Xue Gong Cheng. 26:38–42. 2013.(In Chinese).

19 

Fan XM, Zhang SB, Liu CT, Xiong B and Wang ZL: STAT1 antisense oligonucleotides inhibit secretion of TNF-alpha, IL-8 and NO in alveolar macrophages of rats suffering from interstitial pulmonary fibrosis. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 22:487–489. 2006.(In Chinese). PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ding W, Zhang XY, Pan M, Zhao B, Chen C, Niu ZH, Huang CL, Li YY, Fan XM, Ma YM, Ma YM, et al: Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis. Exp Ther Med 10: 491-497, 2015.
APA
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z. ... Zhang, W. (2015). Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis. Experimental and Therapeutic Medicine, 10, 491-497. https://doi.org/10.3892/etm.2015.2564
MLA
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z., Huang, C., Li, Y., Fan, X., Ma, Y., Zhang, M., Zhang, W."Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis". Experimental and Therapeutic Medicine 10.2 (2015): 491-497.
Chicago
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z., Huang, C., Li, Y., Fan, X., Ma, Y., Zhang, M., Zhang, W."Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis". Experimental and Therapeutic Medicine 10, no. 2 (2015): 491-497. https://doi.org/10.3892/etm.2015.2564
Copy and paste a formatted citation
x
Spandidos Publications style
Ding W, Zhang XY, Pan M, Zhao B, Chen C, Niu ZH, Huang CL, Li YY, Fan XM, Ma YM, Ma YM, et al: Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis. Exp Ther Med 10: 491-497, 2015.
APA
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z. ... Zhang, W. (2015). Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis. Experimental and Therapeutic Medicine, 10, 491-497. https://doi.org/10.3892/etm.2015.2564
MLA
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z., Huang, C., Li, Y., Fan, X., Ma, Y., Zhang, M., Zhang, W."Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis". Experimental and Therapeutic Medicine 10.2 (2015): 491-497.
Chicago
Ding, W., Zhang, X., Pan, M., Zhao, B., Chen, C., Niu, Z., Huang, C., Li, Y., Fan, X., Ma, Y., Zhang, M., Zhang, W."Interleukin‑17A promotes the formation of inflammation in the lung tissues of rats with pulmonary fibrosis". Experimental and Therapeutic Medicine 10, no. 2 (2015): 491-497. https://doi.org/10.3892/etm.2015.2564
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team