Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes

Ye,Li‑Fang; Zheng,Ya‑Ru; Wang,Li‑Hong

doi:10.3892/etm.2015.2662

Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes

Authors:
- Li‑Fang Ye
- Ya‑Ru Zheng
- Li‑Hong Wang
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Affiliations: Department of Cardiology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Cardiology, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China
Published online on: July 28, 2015 https://doi.org/10.3892/etm.2015.2662
Pages: 1348-1354
Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to determine whether the myocardial protective function of Shenmai injection (SM) during ischemia/reperfusion (I/R) is attributable to its regulation of intracellular calcium (Ca2+) and phospholamban (PLB) levels. Cultured neonatal Sprague Dawley rat cardiomyocytes were used to compare the effects of normoxia, total saponins of Panax ginseng (TSPG), ginsenoside Rg1 (Rg1) and SM treatments in rat myocardial cells following I/R. For each of these treatment groups, the mRNA and protein levels of PLB and the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) were evaluated, in addition to the cytoplasmic Ca2+ concentration [Ca2+]i and the rate of apoptosis. The results indicated that I/R markedly decreased phosphorylated PLB and SERCA expression and that SM was able to mitigate this effect, while TPSG and Rg1 were not. Furthermore, SM appeared to prevent aberrant apoptosis and restore the depleted [Ca2+]i resulting from I/R. The protective efficacy of SM against heart disease following I/R may, therefore, be due in part to its effect on intracellular Ca2+ homeostasis. SM may exert its protective effects by relieving PLB inhibition, and the pharmacodynamic actions of SM appear to be significantly more effective than those of its bioreactive components, TPSG and Rgl.

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