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Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway

  • Authors:
    • Yan‑Gui Wang
    • Tian‑Lun Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1777-1783
    |
    Published online on: September 10, 2015
       https://doi.org/10.3892/etm.2015.2741
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Abstract

Recent studies have suggested that liraglutide could have a potential function in improving non‑alcoholic fatty liver disease (NAFLD); however, the underlying molecular mechanism remains unclear. The aim of the present study was to investigate the role of the AMP‑activated protein kinase (AMPK)/sterol regulatory element binding protein 1 (SREBP1) pathway in mediating the effect of liraglutide in reducing fatty degeneration in an in vitro NAFLD model. To resemble the NAFLD condition in vitro, L‑02 cells were treated with 0.5 mM free fatty acids (FFAs) for 24 h. Liraglutide could affect the expression of AMPKα1, phosphorylated AMPKα1 and SREBP1 in a dose‑dependent manner in FFA‑exposed L‑02 cells, as demonstrated by western blot analysis. The intracellular lipid accumulation was significantly decreased, as shown by oil red O staining. A significant decrease in the content of triglyceride and total cholesterol was observed when the FFA‑exposed L‑02 cells were incubated with liraglutide. In addition, the increased expression of liver‑type fatty acid‑binding protein in FFA‑exposed L‑02 cells was suppressed by liraglutide. These effects were reversed by compound C, an AMPK inhibitor. In conclusion, this study has demonstrated that liraglutide can reduce fatty degeneration induced by FFAs in hepatocytes, and this effect may be partially mediated by the AMPK/SREBP1 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang YG and Yang TL: Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10: 1777-1783, 2015.
APA
Wang, Y., & Yang, T. (2015). Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Experimental and Therapeutic Medicine, 10, 1777-1783. https://doi.org/10.3892/etm.2015.2741
MLA
Wang, Y., Yang, T."Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway". Experimental and Therapeutic Medicine 10.5 (2015): 1777-1783.
Chicago
Wang, Y., Yang, T."Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1777-1783. https://doi.org/10.3892/etm.2015.2741
Copy and paste a formatted citation
x
Spandidos Publications style
Wang YG and Yang TL: Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10: 1777-1783, 2015.
APA
Wang, Y., & Yang, T. (2015). Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Experimental and Therapeutic Medicine, 10, 1777-1783. https://doi.org/10.3892/etm.2015.2741
MLA
Wang, Y., Yang, T."Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway". Experimental and Therapeutic Medicine 10.5 (2015): 1777-1783.
Chicago
Wang, Y., Yang, T."Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1777-1783. https://doi.org/10.3892/etm.2015.2741
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